白眉蝮蛇 的英文怎麼說
中文拼音 [báiméifùshé]
白眉蝮蛇
英文
agkistrodon halys ussuriensis-
Its amino acid sequence exhibits significant homology with those of other thrombin - like snake venom enzymes. based on the homology, the catalytic residues and disulfide bridges of gloshedobin were deduced to be as follows : his43, asp88, ser182 and asp176 ; and disulfide bridges, cys7 - 141, cys 28 - 44, cys 76 - 234, cys120 - 188, cys152 - 167 and cys178 - 213
以同樣的方法，得到長白山白眉蝮蛇（ gloydiusussuriensis ）毒類凝血酶基因，並比較了它與大連蛇島蝮蛇類凝血酶基因序列的差別，發現兩者的同源性高達98 ，只有三個氨基酸不同： gln ~ （ 66 ）Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
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