白蛋白測定計 的英文怎麼說
中文拼音 [báidànbáicèdìngjì]
白蛋白測定計
英文
albuminoscope- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 定 : Ⅰ形容詞1 (平靜; 穩定) calm; stable 2 (已經確定的; 不改變的) fixed; settled; established Ⅱ動詞...
- 計 : Ⅰ動詞1 (計算) count; compute; calculate; number 2 (設想; 打算) plan; plot Ⅱ名詞1 (測量或計算...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
- 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
-
The researchers combined proteomics ( the study of proteins ), the latest in mass spectrometry technology and the best analytical methods from the field of bioinformatics ( the use of computers and statistics to analyze and find patterns in scads of data )
研究者將蛋白組學(研究蛋白質) ,最新的質譜測定技術及最好的生物信息學分析方法(應用電腦及統計學分析並檢驗大量數據類型)相結合。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面,用於定向固定htk單克隆抗體,成功研製了檢測htk的壓電石英晶體傳感器,並基於標準scatchard繪圖法,計算出免疫反應的親和常數為1 . 85 106l / mol ,證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術,設計了的檢測htk的質量放大壓電石英晶體傳感器,該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測,應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定,實驗結果為癌癥的臨床診斷與治療提供了參考。The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物,首先克隆了鈍頂節旋藻( arthrospiraplatensisfachb341 )藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列( pc - igs )並進行序列測定,然後根據此測序結果設計引物,通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段,其中包括藻藍蛋白亞基基因( cpcb , 519bp ) ,亞基基因( cpca , 489bp ) ,連接蛋白h基因( cpch , 357bp ) ,亞基基因上游啟動子序列( 427bp )以及各基因之間的間隔區( pc - igs , 111bp ; cpch與cpca間隔區, 184bp ) 。In vitro endothelial proliferation inhibiting activity of recombinant angiostatin was examined with mtt method by using human umbilical vein endothelial cells ( huvec ). in this test we could draw the inhibition curve and calculated the ic50 to confirm its bio - activity further we do cam vascular inhibition test in vivo
4驗證重組蛋白angiostatin的生物活性:採用mm法測定重組蛋白對原代培養的人臍靜脈內皮細胞( huvec )的抑制作用,繪出抑制曲線,並計算出ic 。A pair of primer, dig labeled probe and a taqman probe based on the conserved nucleotide sequence of cp gene of different pnrsv strains were designed and synthesized
本文根據病毒各株系外殼蛋白基因的保守序列,設計了雜交誘捕探針、 dig標記雜交探針以及確定了最佳誘捕參數和雜交檢測參數,建立雜交誘捕rt - pcr ? elisa 。In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in
又將生物信息學技術同實驗技術相結合,針對ftsz保守區設計引物擴增出一條衣藻ftsz片段,進行est搜索、比對、拼接,最終克隆出新基因crftsz1 ;連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索,建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching
第一部分:等離子點ph ( 5 . 4 )條件下,用平衡透析法和紫外光譜,熒光光譜,共振散射光譜研究了ag ( )與人血清白蛋白( humanserumalbumin ,簡稱hsa )或牛血清白蛋白( bovineserumalbumin ,簡稱bsa )的結合。 scatchard圖分析表明, ag ( )在hsa或bsa中有強弱兩類結合部位,通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag ( )與hsa或bsa的結合存在滯後效應,表明ag ( )與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化( a - b ) ,測得並討論了這一構象變化的速度常數和活化參數。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點,設計合成一對特異引物,在pt7 - 7 - rpf4表達質粒的基礎上,應用聚合酶鏈式反應( pcr )對其cdna進行改造,通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ,用快速pcr檢測法、 dna測序分析,鑒定重組hpf4表達質粒的正確性。Caseins and caseinates. determination of lactose content. photometric method
酪蛋白和酪朊酸鹽.乳糖含量的測定.光讀計法The success of human genome project makes the number of protein sequences entering into data bank rapidly increasing. theoretical method computing for predict - ing the structure and function of protein and guiding the experiments is very significative work
隨著人類基因組計劃的順利進展,越來越多的蛋白質序列被測定出來,利用理論計算方法來研究蛋白質的結構和功能從而指導實驗是一項非常有意義的工作。Kjeldahl animal feeding stuffs - determination of nitrogen content and calculation of crude protein content - kjeldahl method
動物飼料.含氮量的測定和粗蛋白含量的計算.基耶達爾Animal feeding stuffs - determination of nitrogen content and calculation of crude protein content - block digestion steam distillation method
動物飼料.含氮量的測定和粗蛋白含量的計算.分塊分解蒸汽蒸餾法It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction
本實驗為重點研究反式高爾基體在毒素細胞內轉運過程中的作用,我們設計了一個反式高爾基體的靶向信號肽基因yqrl ( tyr - gln - arg - leu )連接在rta分子的羧基端,用原核表達蛋白體系,純化並測定rta - yqrl與rta蛋白對hela (人宮頸癌細胞) , wish (人羊膜上皮細胞) , skov - 3 (人卵巢癌細胞)三種細胞的毒性作用。Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因,對位融合於多角體蛋白n端116個氨基酸基因序列的下游(命名為ph - egf ) ,並在兩段基因間設計了凝血酶xa蛋白酶切位點,經過酶切、測序等鑒定正確后,克隆至pbacpak8中,使ph - egf融合基因置於多角體蛋白( polyhedrin , ph )基因啟動子控制之下,構建成重組轉移載體pbacph - egf 。The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表達文庫在一次永久保存基因資源的同時,可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因,進而克隆和表達這些基因,對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律,對揭示其抗藥性分子機理,搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制,對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。Animal feeding stuffs - determination of nitrogen content and calculation of crude protein content - part 2 : block digestion steam distillation method
動物飼料.氮含量的測定和粗蛋白質的計算.第2部分:塊分解蒸汽蒸餾法Animal feeding stuffs - determination of nitrogen content and calculation of crude protein content - part 2 : block digestion steam distillation method iso 5983 - 2 : 2005 ; german version en iso 5983 - 2 : 2005
動物飼料.氮含量的測定和粗蛋白質的計算.第2部分:塊Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體,經dna測序鑒定正確后,溫控誘導表達,獲得了高效表達,表達產物經505一隊ge分析,分子量為16000oa ,符合預計的融合蛋白分子量,表達產物. ll 』菌體總蛋白的30 %以上, westem一blotting結果顯示,該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應,表明我們成功構建了eohistatin的高效融合表達克隆。Methods : a retroviral vector containing vegf gene was constructed and it was expressed after it was transferred to bmsc. the protein expression was detected by immuohistochemistry and its effect of stimulating angiogenesis was observed by microvessal count ( mvc )
方法:構建含vegf基因的逆轉錄病毒表達載體,通過感染兔bmsc使其獲得穩定表達,使用免疫組化的方法檢測其蛋白表達,並通過新生血管計數觀察其促血管生成作用。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白( f )基因裂解位點的核苷酸序列與其毒力的相關規律,分別設計合成了四條寡核苷酸引物,建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應( rt - pcr )技術。分享友人