Objective to investigate the effects of fluid shear stress on il - 8 gene in human umbilical vein endothelial cells ( huvecs ) and the roles of time course of low shear stress and intensity of fluid shear stress using lightcycler ? system ; to investigate the gene expression profiles in huvecs exposed on low shear stress ( 4. 20 dyne / cm2, 2 h ) and incubated by 17 p - estradiol ( 10 - 7 m ) + low shear stress ( 4. 20 dyne / cm2, 2 h ) using the cdna microarray approach. methods endothelial cells were isolated from human umbilical cord veins by collagenase treatment as described by jaffe and modified
目的探討人臍靜脈內
皮細胞（ humanumbilicalveinendothelialcells ， huvecs ）在流體切應力作用下il - 8
基因的誘導表達以及切應力的作用時間和作用強度對il - 8
基因表達影響的變化規律；
利用表達譜
基因晶元技術研究內
皮細胞在低切應力（ 4 . 20dyne cm ~ 2 ， 2h ）作用下其切應力相關
基因的表達情況以及生理濃度（ 10 ~ （ - 7 ）的17 -雌二醇孵育內
皮細胞48h ，再經同樣條件的切應力處理后對內
皮細胞切應力相關
基因表達的可能影響。
Dna extraction from different specimens is the foundation of conservation genetics. it is difficult to extract total dna from tannage of chinese alligator with usual method
利用不同的實驗材料提取適合研究的dna是保護遺傳學研究的
基礎，而對于鞣製
皮革，用常規的dna提取方法很難提到適合於研究的dna樣品。
In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either
本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製
皮革中提取了總dna ，同時對不同的組織標本如鱗片、鹽腌生
皮及尾尖
皮等進行了dna提取：並
利用12srrna
基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測序，以檢驗提取效果。
Described here is the cloning and characterization of a new trichome - specific - promoter in arabidopsis. by ddrt ( differential display reverse transcription ) - pcr and reverse northern, a 280bp sequence is acquired from the epidermal in viciafaba, which is specially expressed in the epidermal. then using race, we abtain a 3. 0 kb gene segment including the 3 ' end full sequence and the 5 ' end partial sequence of the 280bp segment
首先，我們
利用差異顯示技術和反northern技術，以蠶豆葉肉細胞為對照，從蠶豆葉片表
皮細胞中克隆出長280bp的新的表
皮特異表達
基因片段，再通過race技術獲得此
基因片段的3 』端全序列和5 』端部分序列。
A match that rossi ' s men will have to play without peruzzi and oddo ( who just started training yesterday ) and without the other injured players inzaghi, piccoli and baronio
這場比賽將缺陣老佩和四環（昨天才開始訓練） ，其他因扎
基和
皮克
利、把羅尼奧也將缺陣。
October 16, 2007 ( chicago ) ? treatment of basal cell carcinoma ( bcc ) with the pulsed dye laser may be an effective alternative to mohs micrographic surgery if the diameter of the lesion is less than 1. 5 cm, according to a study presented at the american society for dermatologic surgery ( asds ) 34th annual meeting
2007年10月17日? ?根據美國
皮膚外科學會第34屆年會公布的一個研究結果，當損傷范圍直徑小於1 . 5厘米的時候，
利用脈沖機關治療
基底細胞癌的方法對于傳統的顯微外科是一個有效的替代選擇。
We utilized microarray and real - time quantitatie polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement
我們
利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍
皮層的
基因差異表達進行了鑒定。
We utilized microarray and real - time quantitative polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement
我們
利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍
皮層的
基因差異表達進行了鑒定。
Construction of plant expression vector with human endostatin gene and transformation of tobacco
利用人內
皮抑素
基因構建植物表達載體及對煙草的遺傳轉化
The buildings put up and decorated by the school ' s professors ( walter gropius, hannes meyer, laszlo moholy - nagy and wassily kandinsky ) launched the modern movement, which shaped much of the architecture of the 20th century
由該學派的教授們（沃爾特?格羅
皮斯，漢斯?梅爾，拉斯羅?摩
利那
基和韋斯
利?坎丁斯
基）修建和裝潢的一系列建築代表了"現代風格運動"的興起，同時也為20世紀建築發展奠定了
基礎。
In our study, we have applied the mouse bone marrow endothelial cell - conditioned medium ( mbmec - cm ) to promote hematopoietic differentiation of mouse es cells, in order to eliminate contamination of exogenous cells and provide experimental basis on inducing human es cells differentiation into hematopoietic cells
本課題探討
利用骨髓內
皮細胞條件培養液（ mbmec - cm ）誘導小鼠es細胞向造血細胞分化，以消除外源性細胞污染，為誘導人es細胞生成造血細胞提供實驗
基礎。
Abstract : in this paper the histological structure chara cteristics of fur and skin of otter rabbit were studied by optical microscope a nd sem
文摘:
利用光學顯微鏡和掃描電子顯微鏡研究了獺兔
皮毛被及
皮板的組織結構，
基本上探明了獺兔
皮毛和
皮板的組織構造特點。
It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating
抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和
基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性
皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有
利於限制病情的發展，促進損傷的修復。
Total rna was extracted from the second stage larve of hypoderma sp, the 713bp of hypodermin c ( hc ) and 700bp of hypodermin a ( ha ) gene were amplified by rt - pcr 2
從
皮蠅二期幼蟲中提取獲得總rna ，
利用rt - pcr技術擴增獲得了大小為713bp的hypoderminc和大小為700bp的hypodermina
基因。
Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas. its latex is the unique source of crude rubber used in current industry. because of its special and important use, the rubber tree has been extensively planted in tropical areas. increase production is always the main target in rubber tree cultivation. since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant, not only the latex production had been increased largely, but also a new set of rubber tapping system had been established, leading to a series of economic benefit. owing to ethrel " s extensive application, its side effects had been found more and more obviously, such as tapping dry, speeding up senescence, shortening the life span of rubber tree etc. in order to overcome the side effects and increase production more availably, for a long time, people had carried out lots of research work on cell level, membrane level, physiology and biochemistry of laticifer contents. but the mech anism why ethrel increased latex production was not yet understood completely. this study had cloned the ethylene receptor gene ( efrl ) from rubber tree, and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level. the results were as follows : 1
但是，由於乙烯
利應用的普及，乙烯
利刺激割膠引起橡膠樹發生死
皮病及加速膠樹衰老，縮短膠樹壽命等副作用也越來越明顯。為了克服這些副作用，使乙烯
利能更有效地刺激增產，長期以來，人們在細胞水平、膜水平和乳管細胞內含物的生理生化層面上進行了大量的研究，但仍未完全了解乙烯對膠樹的作用機制。本研究從分子水平入手，克隆橡膠樹的乙烯受體
基因（ etr1 ） ，並在轉錄水平和蛋白質翻譯水平上研究etr1
基因在乳管細胞中的表達及與乙烯刺激的關系，取得了以下結果： 1
Z which involve in melanin, tyrosinase related protein 1 gene ( tyrpi ) and dermal melanin inhibitor gene ( id ). the genomic structure of tyrp1 was determined and its correlation between melanin accumulation and tyrp1 expression was studied. moreover, we constructed a bac contig near id locus which was on a region short of dna marker in chr. z
利用構建的bac文庫對z染色體上黑色素相關
基因酪氨酸酶相關蛋白1
基因（ tyrp1 ）的
基因結構以及該
基因的表達與黑色素沉積的關系進行了研究，同時構建了位於z染色體上缺乏標記區段的表
皮黑色素抑制因子
基因（ id ）的bac重疊群。
The reducing sugar content in the phloem and xylem of populus pseudo - simonii, p. pseudo - simonii p. nigra, p. simonii p. nigra, p. koreana, 27 and 40 - year - old p. simonii wood was analyzed by 3, 5 - dinitrosalicylic acid colorimetry
摘要
利用3 ， 5 -二硝
基水楊酸比色法測定小青楊、小青黑楊、小黑楊、香楊、 27年生和40年生小葉楊韌
皮部和木質部的還原糖含量。
Mitogen - activated protein ( map ) kinase signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus, and the increasing evidences showed that mapks are involved in aba -, sa - or h2o2 - signaling respectively. in addition, plant guard cells have been a well - developed model system for understanding how components interact within a signaling network in a single cell
本實驗在表
皮生物分析的
基礎上，主要
利用顯微注射技術、膜片鉗技術和激光共聚焦顯微技術，運用專一性蛋白激酶抑制劑處理，探索蛋白激酶對蠶豆（ viciafabal . ）氣孔保衛細胞中aba和sa誘導的h _ 2o _ 2產生及其信號轉導影響機理，結果如下： 1
The chair of the resident indeed stood vacant before the hearth but on either flank of it the figure of bannon in explorer s kit of tweed shorts and salted cowhide brogues contrasted sharply with the primrose elegance and townbred manners of malachi roland st john mulligan
壁爐前的主席那把椅子是空著的，兩邊分別為身穿探險家派頭的花呢短褲腳蹬生牛
皮翻毛靴子的班農，還有與他形成鮮明對照的瑪拉
基羅蘭聖約翰穆
利根那淡黃色的優美服裝和一派城市的舉止教養。
2 heterogeneity and evolution of 5s rdna pattern of intragenomic and interspecific variation of 5s rdna hi five pines ( including subgenus strobus and pinus ) were studied through cloning and sequencing multiple 5s rdna copies the length of 5s rdna unit is 658 - 728 bp hi diploxylon pines while 499 - 521 bp hi the haploxylon p. bungeana
Ssif7 ： na的序列變異與分子進化利用分子克隆和dna測序分析了油松、雲南松、馬尾松、白皮松和不同遺傳背景的高山松居群的ssilna基因序列變異及基因進化規律，得到以下主要結果： （ 1 ） ssrdna的結構特徵。
