盒細胞 的英文怎麼說
中文拼音 [héxìbāo]
盒細胞
英文
cell-
The last big question on hollis ' s research agenda - - whether the cells will reset after having fired - - may not even matter in the group ' s latest vision for a handheld biosensor : a proposed optical - electronic box would read the photons emitted by a swappable and disposable biosensor chip, which would cost just a few dollars
在霍利斯的研究日程表上，最後一個大問題在於細胞在發光后是否能夠重新設定，按照研究小組最近對手動探測儀的設想，這個問題可能並不太重要：人們擬利用光電盒讀取可交換的一次性生物探測晶元發出的光子，而光電盒只需要花費幾美元。He had short time training course in university of vrije of brussels and nih on homeobox gene lim family study. main fields : neural protection and regeneration. special research interests : neurotrophic factors
近年來，著重於1神經營養因子2同源盒基因lim家族3低氧等所致腦損傷機制及其干預措施4神經肌肉乾細胞發育分化與組織工程等方面的研究。All different cyclins found in mammalian cells contain a 100 amino - acid homologous region named cyclin box, which is the molecular structure marker of cyclins. the expression level of cyclins shows cell cycle phase specialty. it can be regulated by transcription and the degree of protein hydrolysis
在哺乳動物細胞發現的12型細胞周期蛋白中均含有一段由100個左右氨基酸組成的保守區，稱為周期蛋白盒（ cyclinbox ） ，它是細胞周期蛋白的分子結構標志。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。The products cover the fields of the study of molecule biology, expression of protein, from cell culture to layer purification, from antigen antibody, the whole set reagent kit to the microorganism culture and the research of theactivity of the biology
其產品覆蓋了生命領域從分子生物學研究、蛋白表達、細胞培養到層析純化，從抗原抗體、成套試劑盒到微生物培養及其生物活性研究等相關試劑幾近所有過程。Two hours later, according to the protocol of the sabc kit of boster company, an immunocytochemistry was done to examine whether there is neuron - specific proteins in cytoplasm
5小時後用含2 dmso的誘導液處理5個小時。取誘導10小時后的細胞，參照博士德公司的sabc試劑盒操作方法進行免疫細胞化學實驗。Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。分享友人