盤黴素 的英文怎麼說
中文拼音 [pánméisù]
盤黴素
英文
panmycin-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明： （ 1 ）篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ （ 600 ）為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基（含20 mol l乙酰丁香酮）上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。
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