瞬時轉染 的英文怎麼說
中文拼音 [shùnshízhuǎnrǎn]
瞬時轉染
英文
transient transfection-
We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0
我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization
我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation
採用農桿菌浸潤法（ agro - infiltration ）感染轉基因本生煙16c ，並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer. after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes. cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively
以脂質體為介質瞬時轉染健康人及sle患者b細胞系，利用流式細胞技術檢測膜cd40的表達情況，並利用mtt比色法檢測細胞的增殖能力， elisa法檢測培養液的ig濃度，以研究b細胞在cd40被抑制以後增殖能力、抗體分泌的改變。The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7
瞬時轉染cos刁細胞后，通過熒光顯微鏡觀察、間接免疫熒光檢測、免疫組化檢測證實了scfv融合蛋白的表達。The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
將所構建的單鏈抗體基因克隆入綠色熒光蛋白融合表達載體pegfp - n3 ，瞬時轉染cos - 7細胞，分析其表達情況。分享友人