石蠟包埋 的英文怎麼說
中文拼音 [dànlàbāomái]
石蠟包埋
英文
paraffin embedding-
Material and methods normal rats of male sd were divided into young, adult, and aging groups. preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0. 5ml / 100g body weight ). perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart, the whole procedure was lasted for about somin. the entire brain was dissected out and dipped in the fixative solution for 12h at 4. brain pieces targeted were choosen and then passed the graded alcohols for dehydration, dipping into paraffin for embeding, and reshaping the pieces
2 ）磷酸緩沖液100400m ， 30分鐘灌注完畢，取出整腦，在上述固定劑oc ）內后固定12小時。切取觀察部位腦塊，然後，進行梯度酒精脫水，浸蠟，包埋，修塊，石蠟連續切片（德國leica石蠟切片機人切片厚度still ， zlllll ，蛋自甘油載片撈片， 60c烤箱過夜，二甲苯脫蠟，梯度酒精置換，浸水， h六染色，梯度酒精脫水，二甲苯透明，中性樹脂封片。室溫風干后，顯微鏡觀片， olympus萬能顯微鏡照相。In this paper, the methods that the author used are as follows : light microscopy : the testis was fixed in bouin ' s fluid, dehydrated in an ethyl alcohol series, embedded in paraffin, sectioned at 6 u m and stained with hematoxylin and eosin, then observed with olympus microscopy and photographed
光鏡樣品以bouin ' s固定液固定，系列酒精脫水，石蠟包埋，切片厚度6 m ，蘇木精、伊紅染色， olympus顯微鏡觀察並拍照。Collection and preservation of samples : as soon as the three vital signs disappeared, the dogs were anatomized, and the heart, liver, kidney, spleen, lung, brain, muscle in the injection location and no injection location, the heart blood, urine, bile, cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space, spinal cord ( medulla oblongata, cervical cord, the upper beast spinal cord, breast spinal cord and waist spinal cord ) were taken out, some of which were preserved at - 20 for qualitative and quantitative analysis, and the others were fixed with 4 % formaldehyde for the pathology observation
3 、樣品採集：當心電、血壓和呼吸全部消失時，迅速解剖動物，採取心臟、肝臟、腎臟、脾臟、肺臟、大腦、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、膽汁、側山西醫科大學碩士學位論文腦室腦脊液、脊髓腔腦脊液和不同節段的脊髓（包括延髓、頸髓、上胸部脊髓、胸部脊髓和腰部脊髓）等組織，冷凍保存。 4 、病理觀察:採取心臟、肝臟、 』腎臟、脾臟、肺臟、大腦、脊髓等組織， 4 %甲醛固定，石蠟包埋，切片， he染色，光鏡觀察。Fixed with gluteraldehyde, stained with ph 7. 4 phosphate buffered x - gal dye solution, observed by reverse microscope, the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h ， 12h ， 16h ， 20h提取中腸組織，按常規石蠟包埋切片進行洗滌、透明、浸蠟、包埋、切片、粘片等操作程序，製成連續切片，在倒置顯微鏡下逐一進行觀察計數。Each of musculature tissue according to 10 % formalin fixation time of 24h, 48h, 72h, 3m dividing into groups separately, embed by the paraffin wax, cut thick to into slices of 5 u. m, each sample make routine he dye contrast
同時酚/氯仿法常規提取dna 。每例肌肉組織分別按照10福爾馬林固定24h 、 48h 、 72h 、 3個月分組。石蠟包埋，切5卜m厚切片數張。But some cases often require us to examine the special material tissue what had been treated by paraffin wax and formalin fixation
法醫實踐中某些案件卻經常要求我們對送檢的特殊檢材?福爾馬林固定后的人體組織（石蠟包埋塊或病理切片）進行同一認定和個人識別。Discussion in practice of the forensic material evidence, one that is chemical disposal special material to examine, for example material treated by formalin fixation and paraffin wax is needed to distinguish the source to identificate. because the formalin influence dna to be drawn directly, fixation time is the important variation factor among them
討論在法醫物證實踐中，經過化學處理的特殊檢材，例如福爾馬林固定后的石蠟包埋組織塊和切片的個人識別和來源鑒別，因為福爾馬林介導的甲基化直接影響dna提取的效率，其中固定時間是一個重要的變異因素。One full - thickness skin wound ( 6cm2 surface area ) was made on the back
石蠟包埋，進行常規石蠟切片，厚度為54m 。Using colophony - paraffin ( cp ) embedding tissue section technique and immunohistochemical streptalidin - peroxidase ( sp ) method, we have investigated the distribution of 5 - ht neurons in the brain of camponotus japonicus ( big worker ant ), compared the immunoreactivity with gaba, and primarily discussed the character of 5 - ht distribution. the results show that 5 - ht immunoreactive processes originate from a relatively small number of cell bodies but each neuron has processes over a large volume of the neuropil of the brain
本實驗採用樹脂石蠟（ cp ）組織包埋切片技術和鏈黴菌抗生物素蛋白-過氧化物酶（ sp ）免疫組織化學方法，研究了5 - ht能神經元在日本弓背蟻（ camponotusjaponicus ）大工蟻腦中的分佈情況，並與gaba的分佈進行了比較，初步探討了5 - ht在各個腦區的分佈規律和特點。Experiment method the immunostaining procedure followed the sabc technique, as follows : tissue sections were deparaffinised and endogenous peroxidase activity was inhibitioned by 3 % hydrogen peroxide aqueous solution for 20 min
對照組大鼠取與挫傷大鼠相同部位同等大小皮膚組織作為對照。 4多聚甲醛pbs固定后石蠟包埋，連續5 m厚度切片。Cytochemical methods : the testis was fixed in cold acetone, embedded in paraffin, sectioned at 6 u m, observed with olympus bh - 2 microscopy and dna was showed with feulgen, glycogen was showed with pas
細胞化學樣品以冷丙酮固定，常規石蠟包埋，切片厚度為6 m ， feulgen反應顯示dna ； pas反應顯示糖原顆粒， olympusbh - 2型顯微鏡觀察。Embeding the sealing of tissue prepared for permanent microscope slides in a solid block of paraffin wax prior to sectioning
包埋：是在切片之前將組織密封在一小塊石蠟中，以備顯微鏡永久切片之用。For example, when the criminal is released on bail for medical treatment, he would offer the false pathology slices of organize, or others ' malignant tumour pathology information ; and the policyholder offers the fake pathological material while setting a claim, or in the pathology room, we often meet the mark mistake of the sample that is needed to confirm the tissues source of the material. to determine these special material in practice is more and more important
例如：罪犯保外就醫提供的是否本人的病理組織和切片，或者是保險理賠時提供的他人的惡性腫瘤病理切片，或是病理室工作中偶爾遇到標本的標記錯誤，發現與臨床資料不匹配時等案件都需要對這種特殊檢材的組織來源進行鑒定，石蠟包埋組織或者切片的dna分型鑒定在實踐中已經愈發重要。Paraffin embedding apparatus
石蠟包埋器The standard practice of the era was to preserve autopsy specimens in formaldehyde and then embed them in paraffin, so fishing out tiny genetic fragments of the virus from these 80 - year - old “ fixed ” tissues pushed the very limits of the techniques we had developed
當年的標準做法，是將剖檢樣本先以福馬林保存，然後再以石蠟包埋；因此，想要從這些有80年歷史的固定組織中釣出微量的病毒基因片段來，可是得把我們發展出來的技術發揮到極致。分享友人