破碎細胞 的英文怎麼說
中文拼音 [pòsuìxìbāo]
破碎細胞
英文
smudge cells- 破 : Ⅰ動詞1 (完整的東西受到損傷) be broken; be damaged 2 (使損壞) break; break down; damage 3 (使...
- 碎 : 動詞[書面語] (觸; 撞) strike; bump
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 破碎 : 1. (破成碎塊的; 零碎的) tattered; broken 2. (使破成碎塊) smash [break] sth. to pieces; crush
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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This is achieved by breaking up the cells in a mincer or grinder and then centrifuging the resultant liquid
通過研磨器皿或粉碎儀器破碎細胞,然後離心剩餘的液體。Sonic oscillator provides a very efficient means of breaking cells or organelles.
超聲振蕩器為破碎細胞或細胞器提供一種很有效的手段。Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg誘導表達,超聲破碎細胞后,採用親和層析方法純化融合蛋白gst十nadc3 ,並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化,以去除抗gst抗體。Research report on technology of yeast cell disruption
酵母細胞破碎技術的研究Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質The experimental results suggest that the cell disruption ratio can reach 97. 88 % using enzymolysis and nanometer - crushing machine, and the contents of amino acid in the extractives are also considerably raised
試驗結果表明,酵母細胞先經酶解再結合納米破碎機破碎,可使酵母細胞壁的破碎率達到97 . 88 % ,大幅度提高了抽提物中氨基酸的含量。A mononuclear inflammatory cell infiltrate extends from portal areas and disrupts the limiting plate of hepatocytes which are undergoing necrosis, the so - called " piecemeal " necrosis of chronic active hepatitis
單核細胞浸潤從門脈區延伸,破壞正在壞死(也稱為慢性活動性肝炎的「碎片狀」壞死)的肝細胞界板。After breaking up y11 cell by glass beadings, seething solution to remove heat - sensitive proteins, mt was deposited by alcohol
採用玻璃珠破碎細胞,經煮沸除去不耐熱蛋白,再通過乙醇沉澱得到mt粗品。Methods : we took the method of braking cells machinerily, dialysis in low temperature, freeze - drying and son on to enhance its efficacy of oral transfer ring - factor
方法:通過機械法破碎細胞、低溫透析、冷凍乾燥等工藝手段來提高口服轉移因子的藥效。The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only
對於野生菌株的質粒提取,需要選擇合適的提取方法,我們首先採用了細菌細胞質粒提取常用的堿裂解法,但是未取得理想的效果,只得到了許多破碎的質粒dna片段。Impact cell mill
沖擊式細胞破碎裝置The best tissue was the fin. the concentration of colchicines had great effect on the configuration of the chromosomes, and the concentration of 0. 02 % is better. the concentration of the kc1 affected the crash of the cells, and had indirect effect on the dispersion of the metaphases
使用的秋水仙素的濃度對染色體的形態有較大的影響,最適的濃度為0 . 02 mol l ,使用的kcl低滲液的濃度決定著細胞破碎的程度,將對滴片的效果產生間接影響,相對來說,濃度為0 . 0375mol l要比濃度為0 . 075mol l的要好。1 ( + ) / hsblys was trans fected into cos - 7 cells with the calcium phosphate method and the lipofection method. we cultured the recombined cos - 7 cells at optimal condition and harvested cells every 24h. in order to find out the optimal expressive condition, we lysed the cells, extracted the protein, and measure the content of the hsblys
此外,用構建的融合表達載體pcdna3 . 1 ( + ) hsblys轉染真核宿主細胞cos - 7 ,然後在合適的培養條件下進行表達,取不同表達時間( 48h 、 72h和96h )的細胞破碎后進行分析鑒定,找出最合適的表達時間及其他條件。Cwm - f series vibromills are widely applied to crush down any material in fibrin from, high toughness, hardness and with certain water content, especially to the herb processing such as the seed cell wall - breaking, so as to raise the biological utilozation rate of herb, and materials that can not be effctively crush by normal way
本系列振動磨廣泛適用於對各類纖維狀、高韌性、高強度或有一定含水率的物料進行粉碎,特別適用於中藥細胞破壁等旨在提高藥物生物利用度的粉碎要求及用常規方法達到要求細度的物料。The first task in separation of subcellular components is always the rupturing of the cell wall and the protoplasmic membranes.
分離亞細胞組成的第一步總是使細胞壁和原生質膜破碎。Results applications including enzyme catalysis in scf fermentation in scf, scf sterilization and cell disruption were reviewed
結果已在生物工程領域應用的超臨界流體技術有超臨界流體酶催化、超臨界流體發酵、超臨界流體滅菌和細胞破碎技術等。Improving the fermentation technique, cell crash and high efficiency extraction and distillation technology are the prerequisites for commercial production of - carotene
-胡蘿卜素工業化生產的前提是解決好發酵工藝、細胞破碎技術及高效提取純化技術。Klac gene was ligated to the pet - 30a ( + ) vector for expression. then the recombinant plasmid petlac4 was transformed into e. coli bl21. the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm. sds - page analysis and lactase activity assay showed that klac gene was expressed in e. coli, and that the expression level at 28 was much higher than that at 37
表達產物的sds - page分析和酶活性測定表明, klac基因在大腸桿菌中獲得活性表達,其中低溫條件28下的表達水平明顯高於37 , 28誘導的細胞經超聲波破碎所測酶活力為0 . 475u / ml , 37僅為0 . 134u / ml 。Fibroblast covered the wound and joined it. however, in the other groups, inflammatory reaction remains active, and there still were lots of degeneration and necrosis cells
而另兩組仍處于炎癥反應的活躍階段,可見大量的組織細胞變性壞死,細胞破碎,毛細血管內皮腫脹加重。Application of nanometer grade microorganism cell crushing machine in soy sauce production
納米級微生物細胞破碎機在醬油生產中的應用分享友人