硝酸纖維膜 的英文怎麼說

中文拼音 [xiāosuānxiānwéimó]
硝酸纖維膜 英文
nitrocellulose membrane
  • 硝 : Ⅰ名詞（硝石） nitre; saltpetre Ⅱ動詞（用樸硝或硭硝加黃米面處理毛皮） taw
  • 酸 : 酸構詞成分。
  • 纖 : 纖形容詞（細小） fine; minute
  • 維 : Ⅰ動詞1 （連接） tie up; hold together; link 2 （保持; 保全） maintain; safeguard; preserve; keep ...
  • 膜 : 名詞1. [生物學] （像薄皮的組織） membrane 2. （像膜的薄皮） film; thin coating
  • 硝酸 : [無機化學] hydrogen nitrate; nitric acid; aqua fortis
  • 纖維 : fibre; staple; filamentary
  1. The colonies are replica-plated onto nitrocellulose filters.

    將菌落影印接種在硝酸纖維素濾膜上。
  2. The nitrocellulose filters are then placed on wet filter paper containing alkali, which denatures the dna.

    再將硝酸纖維素濾膜置於含堿的濕濾紙上，堿的作用是使DNA變性。
  3. Dab served as chromagen. western blot thirty micrograms of protein extracted from untreated and bfgf, atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane. the blot was probed for nse expression

    4westernblot檢測誘導后細胞的nse表達情況從未經處理和經過bfgf ， atra誘導的細胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝膠上電泳並轉移到硝酸纖維素膜上， 4 5脫脂奶粉封閉過夜。
  4. The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody

    該法以硝酸纖維素膜為固相載體，包被膜載抗原製成的診斷膜片具有良好的特異性：不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應；膜片具有良好的靈敏性，陽性血清作2 ~ （ - 11 ）稀釋亦能檢出；膜片具有良好的穩定性，在4至少能保存7個月，其靈敏性不變。
  5. The nitrocellulose filters are then placed on wet filter paper containing alkali, which denatures the dna

    再將硝酸纖維素濾膜置於含堿的濕濾紙上，堿的作用是使dna變性。
  6. We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane

    樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后，轉印到硝酸纖維素膜上，與第一抗體4孵育過夜，經tbs洗滌后，再與第二抗體室溫孵育2小時， ttbs充分沖洗后，顯色觀察。
  7. Secondly, the grafted membranes were prepared by grafting 2 - acrylamido - 2 - methyl propane sulfonic acid ( amps ) on pretreated membranes using cetric ammonium nitrate ( can ) as an initiator in the aqueous medium. the surface compositions of the grafted membranes were determined by fourier transform adsorption spectrum ( ftir ) and x - ray photoelectron spectroscopy ( xps ). and the morphology of the grafted membranes was studied by scanning electron microscopy ( sem ) and atomic force microscopy ( afm )

    然後，選用硝酸鈰銨作為引發劑， 2 -丙烯酰胺- 2 -甲基丙磺酸（ amps ）為接枝單體，在水溶液體系中進行接枝共聚反應制備pvdf ? g ? amps中空纖維膜，並利用傅立葉紅外光譜（ ftir ） 、 x光電子能譜（ xps ） 、掃描電子顯微鏡（ sem ）及原子力顯微鏡（ afm ）等檢測手段證實了接枝共聚物的存在。
  8. [ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189, the clones were tested by dot blotting and elisa

    並通過硝酸纖維膜斑點印跡法進行陽性克隆鑒定。 elisa分析陽性噬菌體克隆與抗體的親和力。
  9. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
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