磷酸化蛋白 的英文怎麼說
中文拼音 [līnsuānhuàdànbái]
磷酸化蛋白
英文
phosphorylated protein- 磷 : 名詞[化學] phosphorus (15號元素,符號p)
- 酸 : 酸構詞成分。
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 酸化 : acidate; acidulate; acidize; acidification酸化泵 acidizing pump; 酸化過程 acidization; 酸化劑 aci...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature
本文以甘藍葉片為材料,通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究,以期找出甘藍葉片對低溫脅迫的生理生化響應規律,為甘藍露地越冬栽培防範寒害提供理論指導,同時對低溫脅迫下甘藍逆境信號傳導進行了探討,從而為徹底弄清低溫脅迫機理提供進一步的證據,研究的主要結果如下:丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗壞血酸過氧化物酶( asp )和過氧化物酶( pod )活性在低溫處理0 30min發生顯著變化,低溫處理3min后,甘藍葉片內mda含量基本沒有變化,處理5min時出現第一個峰值,達到對照的104 . 10 , 10min出現低谷,僅為對照的86 . 27 ,隨后再次上升, 30min時超過第一峰值,為對照的113 . 93 。At rest, the heterodimeric rel / nf - k b complex is located in the cytoplasm bound to an inhibitory factor, i k b. upon stimulation, i k b is phosphorylated and degraded, free nf - k b then translocates into the nucleus where it binds to k b site to regulate transcription
靜息時, re皿4bh聚體與抑制蛋白kbs結合,在細胞漿中保持無活性。受到刺激, ikb分子迅速磷酸化並降解,釋放nf兒b轉位入核,通過與kb位點結合調節轉錄。For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少,為了研究可逆磷酸化對psii反應中心結構與功能的影響,本文以蕹菜為材料,從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶,進行了酶學性質和光譜性質的研究。Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后, ps活性恢復是一個復雜而有序的過程,需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理,加入林可黴素阻斷葉綠體蛋白質合成,利用尿素sds變性電泳分離類囊體膜蛋白,藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式,並進行定量分析。Effect of cell adhesion on vasodilator - stimulated phosphoprotein phosphorylation
細胞黏附對血管擴張刺激磷蛋白磷酸化的影響Conclusion : phosphoprotein enrichment technique combined with two - dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins
結論:磷酸化蛋白富集技術與二維凝膠電泳技術的結合是研究磷酸化蛋白質組的有效方法,為進一步研究人腎組織磷酸化蛋白奠定基礎。Chloroplast phosphoprotein were first found in thylakoid membranes by bennett o the attachment or removal of a phosphate group from a protein may have profound effects on that protein ' s activities and properties, the reversible phospharylation of the membrane protein in thykaloid is a dymanic equilibrium process
而bennett ( 1977 )發現的類囊體膜蛋白可逆磷酸化已成為近年研究的熱點之一,蛋白質連上或是移去磷酸基團都將強烈影響蛋白質的生理活性和性質,這需要類囊體膜蛋白激酶和磷酸酯酶的參與。Vanadate, a potent ptpase inhibitor, locks adipocyte differentiation at an early stage in the program and leds to the accumulation of p - c - crkii, a phosphotyrosyl protein that is a substrate for ptpase ha2
研究發現一種強烈的磷酸酯酶抑制劑- -釩酸鈉能夠作用於脂肪細胞的分化早期過程從而抑制其分化,並使一種ptpha2的底物即酪氨酸磷酸化的蛋白質p - c - crkii累積。In rat hippocampal slices, tbs induced phosphorylation of p21 - activated kinase ( pak ) and its downstream effecter cofilin, signaling proteins that enable actin filament growth
在鼠海馬切片中,短暫快速脈沖刺激導致蛋白激酶21的磷酸化,在它的下游區產生了cofilin ,一種能使肌動蛋白絲生長的信號蛋白。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eIn thl1 gene, there are some famililar enzyme digest sites including bamh i, hind, sac i and sal i. the sequence of thl1l from brassica oleracea l. shows 99 % identity to the sequence of thll from brassica napus l. there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein
甘藍和油菜thl1基因序列有5個堿基的差異,而氨基酸僅有2個氨基酸的變異,同源性達99 。在thl1蛋白的氨基酸序列中發現了一個硫氧還蛋白家族的活性位點( cppc )和多個磷酸化位點。The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain
精母細胞周圍的囊細胞可能合成scf樣蛋白,特異地識別精母細胞膜上的c - kit受體,並刺激c - kit發生二聚化、自體磷酸化,激活胞內酪氨酸激酶活性,活化具有「 sh _ 2結構域」的靶蛋白,可能通過一系列信號級聯,最終激活與減數分裂的相關蛋白或基因。Studies revealed that p - catenin dissociated from ccc and translocated into free catenin pool in cytosol after it has been phosphorylated at tyrosine or serine residues, and in this situation, the ccc has been disrupted and cell adhesion function disturbed. a large amount of the free p - catenins in the cytosol can be degraded by the tumor suppressor apc, and the remains translocate into nucleus and bind to transcriptional factor tcf / lef in the nucleus and then promote cell proliferation related gene or anti - apoptosis gene transcription
當-連環蛋白酪氨酸或絲氨酸殘基磷酸化后,就與ajs發生解離而游離到細胞漿中,此時細胞的粘附功能也發生障礙,游離到胞漿中的-連環蛋白,一部分被抑癌因子apc降解,一部分則轉移到細胞核內,與核內的轉錄因子tcf lef結合,啟動與細胞增殖有關的基因轉錄。Results : the phosphorylated proteins were successfully extracted from human kidney tissues and were separated by two - dimensional gel electrophoresis
結果:成功提取了人腎組織磷酸化蛋白,並得到了磷酸化蛋白的雙向凝膠電泳圖譜。After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and sds electrophoresis on second dimension
樣品濃縮除鹽后,進行一維等電聚焦分離和二維sds電泳分離提取人腎組織磷酸化蛋白。( 4 ) phosphorylation modulation decreased activitis and temperature sensitivity of bkca channel reversibly, which suggested the participation of protein phosphorylation in the temperature regulation
通道在某一時間點的磷酸化狀態取決于蛋ei激酶的磷酸化、蛋白磷酯酶的之磷酸化之間的平衡。Methods : the phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography ( pmac ) resin
方法:利用金屬磷酸鹽親和層析樹脂富集人腎組織磷酸化蛋白。The self - incompatibility is involved in a signal transduction process resulted from the interaction between ligand and receptor. up to now, some achievements for ligand and receptor have already been made, but the research progress on downstream protein factors is slower than that of upstream components, highlighting the downstream proteins in priority of research. some downstream components have not been isolated, which are similar to the unknown function of those isolated ones
自交不親和反應是一種配體和受體相互識別的信號傳導過程,在這一過程中,對配體和受體的研究已經取得突破性成果,而對下游的磷酸化蛋白研究相對上游受體來說要緩慢得多,所以對信號傳導的下游組分研究顯得尤為重要,特?是下游還有許多未分離到的組分,而分離到的組分其功能還不清楚。It went down quickly in turn, at 15min, it reached the lowest level, but it still be 1. 36 time s of the control, which was much higher than that of the control, then kept rising. the change of aba content shows a coincidence with what described above
通過電泳和放射性標記發現低溫脅迫導致甘藍葉片內迅速出現一種分子量為22 . 9kd的磷酸化蛋白質,這說明甘藍在低溫脅迫條件下的信號傳導過程仍然依賴于磷酸不扮去磷酸化過程,這種磷酸化蛋白非常有可能是該信號傳遞過程中的關鍵物質。分享友人