磷酸化酶 的英文怎麼說
中文拼音 [līnsuānhuà]
磷酸化酶
英文
enzyme phosphorylase-
Effect of filamin - c on extracellular signal - regulated kinase phosphorylation mediated by 1a - adrenergic receptor in hek293 cell
腎上腺素受體介導細胞外信號調節激酶磷酸化作用的影響Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways
我們發現,低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應,並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降, dna聚合酶譜發生改變,應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活,細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇,細胞信號轉導通路camp - pka - creb和jnk sapk被激活。According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature
本文以甘藍葉片為材料,通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究,以期找出甘藍葉片對低溫脅迫的生理生化響應規律,為甘藍露地越冬栽培防範寒害提供理論指導,同時對低溫脅迫下甘藍逆境信號傳導進行了探討,從而為徹底弄清低溫脅迫機理提供進一步的證據,研究的主要結果如下:丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗壞血酸過氧化物酶( asp )和過氧化物酶( pod )活性在低溫處理0 30min發生顯著變化,低溫處理3min后,甘藍葉片內mda含量基本沒有變化,處理5min時出現第一個峰值,達到對照的104 . 10 , 10min出現低谷,僅為對照的86 . 27 ,隨后再次上升, 30min時超過第一峰值,為對照的113 . 93 。Glycogen synthase 5sinweis
糖原磷酸化酶This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column
使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法,從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少,為了研究可逆磷酸化對psii反應中心結構與功能的影響,本文以蕹菜為材料,從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶,進行了酶學性質和光譜性質的研究。Chloroplast phosphoprotein were first found in thylakoid membranes by bennett o the attachment or removal of a phosphate group from a protein may have profound effects on that protein ' s activities and properties, the reversible phospharylation of the membrane protein in thykaloid is a dymanic equilibrium process
而bennett ( 1977 )發現的類囊體膜蛋白可逆磷酸化已成為近年研究的熱點之一,蛋白質連上或是移去磷酸基團都將強烈影響蛋白質的生理活性和性質,這需要類囊體膜蛋白激酶和磷酸酯酶的參與。Plant phosphorylase exhibits several differences from animal phosphorylase.
植物磷酸化酶與動物磷酸化酶比較,顯示幾方面的差異。Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity. rabbits inoculated two times at one week interval
擴增此質粒,回收bamh切出的2 . 5kb的基因片段,與pme290 ( bamh酶切後去磷酸化)載體連接。Vanadate, a potent ptpase inhibitor, locks adipocyte differentiation at an early stage in the program and leds to the accumulation of p - c - crkii, a phosphotyrosyl protein that is a substrate for ptpase ha2
研究發現一種強烈的磷酸酯酶抑制劑- -釩酸鈉能夠作用於脂肪細胞的分化早期過程從而抑制其分化,並使一種ptpha2的底物即酪氨酸磷酸化的蛋白質p - c - crkii累積。In rat hippocampal slices, tbs induced phosphorylation of p21 - activated kinase ( pak ) and its downstream effecter cofilin, signaling proteins that enable actin filament growth
在鼠海馬切片中,短暫快速脈沖刺激導致蛋白激酶21的磷酸化,在它的下游區產生了cofilin ,一種能使肌動蛋白絲生長的信號蛋白。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eThe combination of meja and fc did not show significant additive effect on enzyme activity. 3. phosphorylation and dephosphorlation of pm h ^ - atpase after treatments of meja and fc 1 ) in vitro, phosphatase inhibitors, okadaic acid and cantharidin, enhanced meja - induced increase of the enzyme activity to 60 % and 50 %, respectively
Tllrase過程中可能發生的磷酸化和去磷酸化作用1 )磷酸酶抑制劑斑螫素、崗田酸可以增強meja對酶的刺激作用,崗田酸的效應較強,而斑鰲素的作用略弱,增幅分別為60和50左右。The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain
精母細胞周圍的囊細胞可能合成scf樣蛋白,特異地識別精母細胞膜上的c - kit受體,並刺激c - kit發生二聚化、自體磷酸化,激活胞內酪氨酸激酶活性,活化具有「 sh _ 2結構域」的靶蛋白,可能通過一系列信號級聯,最終激活與減數分裂的相關蛋白或基因。Oocyte maturation involves the activation of various signal trans - duction pathways that converge to activate maturation - promoting factor ( mpf ) ; this is a key activity that catalyses entry into m - phase of meiosis i and meiosis ii
在爪蟾卵中有大量未被激活的cdc2 - cyclinb復合物,稱為pre - mpf , pre - mpf在tyr15位脫磷酸變成活性mpf ,起到這個作用的磷酸酶是cdc25 , cdc25的活性通過磷酸化和細胞位置進行調控。Focal adhesion kinase tyrosine phosphorylation promotes rat hepatic fibrogenesis and its possible mechanism
黏著斑激酶酪氨酸磷酸化促大鼠肝纖維化形成及其可能機制And the intron had a lot of gt repeated sequence. the dna and protein sequence of this gene was analyzed using the bioinformatics tools. two functional domains were found in the protein
運用生物信息學手段對3一磷酸甘油脫氫酶基因核酸以及蛋白質序列做出了分析,發現這個基因編碼兩種功能的結構域,磷酸化酶結構域和3一磷酸甘油脫氫酶結構域。Changes of activities of mlck and dephosphatase in different arterial vessels from hypertensive rats
高血壓大鼠不同血管平滑肌肌球蛋白磷酸化和脫磷酸化酶的變化One is phosphatase - like domain and the other is glycerol - 3 - phosphate dehydrogenase domain. the former domain may catalog the dephosphation of the glycerol - 3 - phosphate and the later presumably synthesize the glycerol - 3 - phosphate according to similarity searching, domain localization and structure comparison. these should give an evidence for the functional research of this gene
通過相似性搜索,結構域定位以及結構比較分析我們認為磷酸化酶結構域很可能是催化3一磷酸甘油水解,而3一磷酸甘油脫氫酶結構域是催化3一磷酸甘油合成的,為這個基因功能的鑒定提供了根據分享友人