移入基因 的英文怎麼說

中文拼音 [yírùjīyīn]
移入基因 英文
transgenom
  • 移 : Ⅰ動詞1. （移動） move; remove; shift 2. （改變; 變動） change; alter Ⅱ名詞（姓氏） a surname
  • 入 : Ⅰ動詞1 （進來或進去） enter 2 （參加） join; be admitted into; become a member of 3 （合乎） conf...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 移入 : brought down
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。
  3. In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method

    為了選育新類型玉米雄性核不育系，提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略，同時應用分期播種的方法為作物提供不同的生長和發育的環境條件，以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明，春播環境下可鑒定和選擇玉米的不育性，夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性，結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性，為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇，能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法，使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系
  4. 6. emsa with coincubation of specific antibodies to the p50 or p65 subunits of nf - b showed a distinct retardation in the mobility with the p65 antibody and a reduction in the intensity of the shifted band with the p50 antibody

    6 ，加入抗nf一kbp65和p50的抗體進行電泳遷移率改變實驗，證實nf一kb形成p50 / 65異二聚體參與mbd3基因的轉錄調節。
  5. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  6. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  7. They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection

    用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。
  8. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  9. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  10. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  11. A fragment, containing 2. 0kb cloned 5. tenebrarius dna and reported genes of erme and xyle, was inserted in plasmid phz132 ( an e. coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4. the plasmid was transformed into e. coli et12567 ( puz8002 ) to construct recombinant e. coli et12867 ( puz8002, pzxb014 )

    將克隆到的鏈黴菌dna2 . 0kb片段以及報告基因erme 、 xyle插入到具有orit的大腸桿菌?鏈黴菌穿梭質粒phz132中構建接合轉移質粒pzxb014 ，並將其轉入大腸桿菌et12567 （ puz8002 ）中，獲得供體菌et12567 （ puz8002 ， pzxb014 ） 。
  12. In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226

    將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。
  13. A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19

    進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因，構建了大腸桿菌啟動子探針載體phn1005 ，該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度；其3 』端含rrnat1t2終止子，可允許克隆強啟動子；在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀； gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止，可保證gfpmut3以正確的讀碼框翻譯。
  14. The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied

    通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。
  15. Topics include ( 1 ) general mechanisms of disease ( inflammation, infection, immune injury, host response to foreign materials, transplantation, genetic disorders and neoplasia ), ( 2 ) pathology of lipids, enzymes and molecular transporters, ( 3 ) pathology of major organ systems, and ( 4 ) review of diagnostic tools from invasive surgical pathology to non - invasive techniques such as optical spectroscopy, functional imaging, and molecular markers of disease

    主題包含（ 1 ）疾病機制通論（發炎，感染，免疫受損，宿主對異物反應，移植，基因異常與癌癥） （ 2 ）脂質、酵素與分子傳送者病理學（ 3 ）主要器官病理學以及（ 4 ）從侵入性手術病理學到非侵入性技術診斷工具的回顧，例如光譜學、功能性影像斷層掃描、和疾病分子標記。
  16. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  17. In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure

    本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。
  18. The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )

    將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。
  19. Using ex vivo gene transfer technique, exogenous gene was introduced to the liver graft during cold preservation and express locally in the graft. the effect of inhibition of rejection and inducing liver graft tolerance was observed. through this study, the possibility of achieving graft tolerance by gene transfer without routine immunosuppressive drugs was explored

    我們採用adeasy腺病毒載體系統，自行構建攜帶huctla4 - ig的重組腺病毒，通過先體外后體內（ exvivo ）基因轉移技術，于供肝冷保存時，將該治療基因導入大鼠移植肝，使其于移植肝局部表達，觀察其抑制排斥反應，誘導大鼠移植肝免疫耐受的作用。
  20. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
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