種子酵母 的英文怎麼說

中文拼音 [zhǒngzijiàomǔ]
種子酵母 英文
seed yeast
  • 種 : 種名詞（姓氏） a surname
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 種子 : seed; semen; germ; grain; stock; spermo ; spermato ; gono 種子測定 seed testing; 種子處理 seed tr...
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  1. The basidiospores proliferate in a manner comparable to the multiplication of yeasts.

    擔孢子以一種可與酵母繁殖相比擬的方式進行增殖。
  2. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  3. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  4. Extracts of 10 selected chinese herb medicines ( rhizoma coptidis, cortex phellodendri, radix scutellariae, fructus forsythiae, cortex magnoliae officinails, radix glycytthizae, folium artemisiae argyi, ligustici chuanxiong rhizoma, flos lonicerae, fructus schisandrae ) were tested in vitro against six different microorganisms [ bacteria ( staphylococcus aureus, escherichia coli and bacillus subtilis ), yeast ( saccharomyces cerevisiae ) and find ( aspergillus flavu and trichoderma viride )

    摘要本文選用黃連、黃柏、黃芩、連翹、厚樸、甘草、艾葉、川芎、金銀花及五味子十味中草藥水提液，對金黃色葡萄球菌、大腸桿菌、枯草芽孢桿菌、啤酒酵母、黃麴黴及綠色木霉這六種菌進行了體外抗菌試驗。
  5. Although yeast cannot digest cellulose or lignin, the molecules that form a plant ' s skeleton, some bacteria and other species of fungi are able to do the job

    雖然那些組成植物莖干纖維素和木質素的分子不能為酵母所消化，但是某些細菌和其他種類的真菌可以完成這項任務。
  6. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  7. We found that there are two types of map1lc3 in rat, mouse and human, besides that, there is another type ( map1lc3c ) in human. we expressed all map1lc3 in hek293 cells respectively and found that the post translational modification of all map1lc3 is similar to yeast apg8 and rat map1lc3 identified except human lc3b. characteristic carboxyl cleavage occurred in conserved glycine of them

    在hek293細胞中分別表達這三個物種中的所有的a型、 b型以及人map1lc3c分子，發現除了人的map1lc3b外其它同源物的修飾方式與已知的酵母apg8和大鼠的lc3修飾方式相類似，均發生了羧基端的切割反應而且羧基端保守的甘氨酸是它們發生翻譯后修飾的活性位點。
  8. He and his colleagues have taken 13 enzymes, derived from fivedifferent organisms ranging from spinach to rabbits viayeast and bacteria, and assembled apathway that converts starch into hydrogen

    他與他的同事們利用酵母菌和細菌從五種不同的有機體內，包括菠菜、兔子等，提取了13種酶，經過組裝后能使澱粉轉化成氫氣。
  9. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  10. Pias1, a protein that was also located in test ' s specifically was got pdz domain protein tight junction protein 2 and pcg ( polycomb group ) family ring1 and polycomb 2 were included

    3kbp （ kyotbindingpotcin ）功能的研究： kbp是我們通過酵母雙雜交獲得的與kyo 』 fz相互作用的一種新分子。
  11. In order to assess the function of kyot2, northern blot, immunohistochemical sabc and in situ hybridization methods were used to find out the expression of kyot and kyot knockout mice which were kept in our laboratory were analyzed. moreover, we employed a yeast two - hybrid screening using kyot2 as a bait protein and got 13 interacting proteins. the function of a novel protein of them, kbp was investigated

    為了進一步研究kyot的功能，我們利用northern印跡、兔疫組織化學等方法對kyot在體內的分佈進行時空定位，並利用本室懈的kyot基困剔除小鼠分析其表型，同時採用酵母雙雜交篩到tkyoth相互作用的13種分子，並對其中的一個新分子kbp進行了功能的研究
  12. Rab proteins are a family of small molecular weight gtpase of ras superfmaily with characteristic molecular weight about 22 - 25 kd. rab gtpases are expressed in almost all eukaryotic cells, ranging from yeast to drosophila and to human. they are ubiquitously or tissue - specifically expressed and function in all of cellular aspects

    Rab蛋白是屬于ras蛋白超家族的小g蛋白（ smallg - proteinorsmallgtpase ） ，分子量大多在22 - 25kda之間，表達于從酵母、果蠅、小鼠到人類等各種真核生物中，發揮著各種各樣的功能；其在酵母中稱為ypt sec4p在高等真核生物中稱為rab 。
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