種系基因組 的英文怎麼說
中文拼音 [zhǒngxìjīyīnzǔ]
種系基因組
英文
germ line genome-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證The experiment was conducted in a 2. 4 ha isolated field mimic rice production practice with pollen competition. a japonica gm rice l201 containing bar gene with herbicide basta resistance was used as a pollen donor and six indica hybrid rice varieties and its male sterile ( ms ) lines and two common wild rice ( oryza rufipogon and o. nivara ) that share same aa genome with cultivated rice were used as recipients
本試驗選擇廣州作為華南水稻生態區的代表,以含bar基因(抗除草劑basta )的轉基因粳稻l201為花粉供體,模擬大田生產實際,對轉基因向秈型兩系及三系雜交稻不育系、雜交稻品種及含aa基因組的普通野生稻的基因漂流及其影響因素進行了研究。Robustness of our results was confirmed by high bootstrap support of all nodes in the trees. this result contradicts the batrachia hypothesis ( a salamander + frog grouping ), and is consistent with bolt ' s hypothesis ( 1991 ) basing on the morphological data. the result was also supported by previous molecular studies based on mitochondrial and nuclear rrna data
這個結果與蛙類假說是相矛盾的,與bolt ( 1991 )中國澤蛙線粒體基因組結構及種群系統地理學研究在形態學基礎上提出的絨蝶類和蚓螺類為姐妹群關系的假說相一致,並得到建立在線粒體和核trna基因數據基礎上的許多分子研究的支持。The complete nucleotide sequence of the mitochondrial genome of f. limnocharis was detailedly compared with those of 5 other amphibians. the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa. using teleosts as outgroup, the phylogenetic analyses results show that mp, nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders
我們在測定了澤蛙線粒體全基因組序列的基礎上,與已知其它的5種兩棲類進行詳細的比較分析,同時選擇了11種高等脊椎動物的線粒體全基因序列,以硬骨魚類做外群,用22個trna基因合併數據進行系統發生重建分析,結果表明mp 、 nj和ml樹都強力地支持現生兩棲類動物為單系群並且蠑螈類和蚓螈類為姐妹群關系(自引導值分別為92 、 99 、 100 ) 。The tl seeds are screening on the ms medium which contains 50ug / ml kan, and there are about 70 % of the transgenic lines showed the kan - resistance with a ratio of 3 : 1. however, the other lines did n ' t show the ratio consistent with the mendelism. it might prove that most of the transgenic plants only have one insert
對收獲的轉基因番茄t _ 1代種子進行卡那黴素抗性篩選,結果發現, 70以上獨立轉化株系的t _ 1代種子出現了3 1的抗性分離,另一些獨立轉化株系的種子並未表現出符合孟德爾遺傳的分離比,說明大部分er - shsp基因是以單拷貝的方式插入植物基因組。In this work, some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique. according to the result of genomic dna amplified with pcr ( polymerase chain reaction ), genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance. a primary but first time research at the phylogenetic relationships of some genera and species, the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics
本文在初步調查分析了棕櫚科植物在我國的自然分佈、引種馴化情況以及該類植物在廈門地區栽培應用狀況的基礎上,首次採用rapd分子標記技術,對廈門萬石植物園引種的一些棕櫚科植物的遺傳多樣性進行了研究,根據pcr對基因組dna擴增的結果,用nei ' s相似性系數計算了不同植物間的遺傳距離和遺傳一致度,通過對遺傳距離的upgma聚類分析,並結合形態分類的特點,對棕櫚科植物的屬、種間的系統分類關系和一些疑難種的分類鑒定進行了初步研究。The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus
本研究的目的在於以人的-干擾素基因( ifn - )作為目的基因,與鯉魚-肌動蛋白基因啟動子在體外重組,利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究,其結合了干擾素和基因工程育種抗草魚出血病病毒的優點,以期獲得對草魚出血病具有天然抗性的轉基因魚,並在此基礎上培育出草魚抗病新品系。Research topics cover the basic and applied parasitology, including the major parasitic groups, pathogenic biology, host specificity, epidemiology, ecology, the control of parasitic infections, and biochemical and molecular parasitology, and ranging from host - parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture
在整體和分子水平上,應用現代生物技術對危害人體和重要經濟動植物的主要寄生蟲(原蟲類、吸蟲類、絳蟲類、動物線蟲類、植物線蟲類及醫學昆蟲等)和由其引起的病蟲害進行研究,在病原生物學(包括生活史、媒介種類、基因組結構與表達調控等) 、流行病學、生態學,寄生動物與宿主間的相互作用關系等研究基礎上,實現對病原物的診斷、治療和控制。The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management
重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系,蚊蟲病原微生物菌種及其基因資源,微生物基因組學和比較基因組學,殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建,新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等,發展新的生物防治技術,建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。Clustering analysis showed that plants in the genus of p. salicina could be distinguished from those in the genus of p. ameniaca ; there was some genetic relationship among p. mume, p. salicina and p. ameniaca, of which p. ameniaca was closer to p. mume in genetic relationship ; and the distance between varieties of each genus was different, with the smallest being 0. 1138 and largest being 0. 7633. the genetic distance reflects genetic relationship between tested materials. the result that varieties of each genus were close to each other in genetic relationship testified the traditional morphology - based taxonomy from the genomic dna
不同引物擴增出的帶型完全不同,聚類分析結果表明,李屬植物和杏屬植物能完全被區分開,李、杏和梅之間表現出一定的親緣關系,其中杏,梅之間的親緣關系較近,各屬品種之間都有不同的遺傳距離,最小距離為0 . 1138 ,最大距離為0 . 7633 ,遺傳距離的大小反映了材料間親緣關系的遠近,各屬內品種的親緣關系比較近,這一聚類結果從供試材料基因組dna分子水平驗證了傳統的形態學分類觀點。Therefore, we hope to construct a effective eukaryotic gene expressing vector harboring a genomic dna, including introns, and develop a gene expressing system could correctly splice the mrna
為此,我們希望構建和探索一種能有效高表達含有內含子真核基因組的載體和能對內含子進行剪切加工的昆蟲表達系統,以提高外源真核基因表達的有效性和可靠性。Thermocycled pcr samples were resolved electrophoretically on 1. 5 % agarose gels and taken photos using a polaroid camera. the statistical results were analyzed by the spss software, and the cluster figures were obtained. conclusions could be drawn from the study as following : 1 ) the molecular systematics of 57 species of crickets, which belong to 26 genera 7 family in grylloidea, had been studied by the approach of rapd
本項研究在依據外部形態分類鑒定及前人工作的基礎上,採用rapd技術,通過對蟋蟀總科7科26屬57種蟋蟀基因組dna的rapd圖譜的比較研究,在分子水平上探討這些類群的分類地位和親緣關系,為豐富蟋蟀總科的分子系統學研究,並為進一步完善蟋蟀總科的分類系統,揭示其系統發育及演化提供分子水平的依據。In this paper, the approach of rapd was used to study 57 species of crickets, which belong to 26 genera, 7 families, in order to discuss their taxonomy status and gentic relationships in molecular level, and enrich the research of molecular systematics in grylloidea. the genome dna of the 57 species were extracted by the method of proteinase k isolation of total dna
蟋蟀總科的分子系統學的研究也主要集中在個別種種內或種間mtdna多態性方面及個別種同工酶的研究上,而利用rapd技術進行蟋蟀基因組dna多態性方面的研究在國內外更少,國外僅見chu , jHere we describe the mapping of peptides derived from accurate interpretations of protein tandem mass spectrometry ( ms ) data to eukaryotic genomes and the generation of an expandable resource for integration of data from many diverse proteomics experiments
本文中我們描述了通過蛋白質串聯質譜的準確注釋數據所得到的肽段與真核生物基因組之間的對應關系,以及用於集成來源於多種不同蛋白質組學試驗的數據的可擴展資源的生成。They say that geographic patterns of sets of genes show that people have been migrating and merging from the start ; race may be heavily tied to culture and how people see one another, but it is something we have created
他們認為,各地域基因組合的規律顯示,人類從一開始就在遷棲、在結合;種族可能與文化及人們怎樣相互看待有緊密的聯系,但這些不過是我們自行建構出來的產物而已。They could be divided into two groups according to phylogenetic tree analysis results, one was the genomic dna of microtus fortis calamorum and the other one was that of microtus fortis fortis. however, the homologues reached up to 98 % between two subspecies
同時採用系統發育樹( phylogenetictree )分析方法,對兩個亞種的親緣關系進行了分析和比較,結果顯示,東方田鼠長江亞種和寧夏指名亞種基因組dna明顯的分為兩大組別。Currently, the research methods can be classified into two main classes, that is, genetic linkage analysis and linkage disequilibrium analy - sis. the two methods explore recombination fraction and linkage disequilibrium coefficient correspondly which are used to measure genetical characters, moreover, statistical methods are used to execute gene mapping
基因定位是人類基因組計劃( hgp )重要目標之一,目前研究方法主要分為兩大類:基因連鎖分析與連鎖不平衡分析。兩種方法分別藉助衡量遺傳性質的重組率與連鎖不平衡系數,並利用統計方法來進行基因定位。In this dissertation, influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied, and thereafter, stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances
本論文在對高羊茅胚性愈傷組織植株再生與農桿菌介導遺傳轉化的多種影響因素進行系統研究的基礎上,將組成型表達啟動子camv35s引導的耐逆相關cbf1基因導入該草種的基因組,獲得耐逆性增強的轉基因植株。Through the aoe centre, our department also participates in the china national programme of rice functional genomics and is responsible for the part of seed quality
而通過這領域,生物系亦獲邀參與中國水稻基因組功能國家重要科研項目,負責種子品質研究。With the appearing of transgenic animal, it is possible that people can rebuild the genome of animals by gene recombination and contrive research on relative gene ' s structure and function in viviperception. this is a multidimension system of gene research with regard to providing new approaches and thoughts from molecule to individual arrangement
轉基因動物的出現,使人類可以通過基因重組的各種方法人為的改造動物基因組,在動物活體水平上研究有關基因的結構和功能,是一個四維時空的研究體系,為從分子到個體的各個層次,多維的研究基因提供了新的方法和思路。分享友人