穩定轉染 的英文怎麼說

中文拼音 [wěndìngzhuǎnrǎn]
穩定轉染 英文
stable transfection
  • 穩 : 形容詞1 （穩定; 穩當） steady; stable; firm 2 （穩重） steady; staid; sedate 3 （穩妥） sure; rel...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 轉 : 轉構詞成分。
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  • 穩定 : 1 （使穩定） stabilize; steady 2 （穩固安定） stable; steady 3 （物質的性能不易改變的作用） stabi...
  1. Stable suppression of afp gel expression by rnai in smmc - 7721 cells

    表達質粒穩定轉染肝癌細胞克隆的構建
  2. The binding characteristics of the opioid ligand

    受體激動劑對穩定轉染
  3. Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome

    前言端粒酶（ telomerase ）是一種核糖核蛋白逆轉錄酶，能以自身的rna為模板，從頭合成染色體末端的端粒dna ，彌補細胞分裂時端粒dna的丟失，維持端粒的長度並穩定染色體。
  4. Compared with traditional coal drying system, the disk dryer is a indirect drying dryer, in which, the material dried not direct contact with drying medium, so the pollution is avoid, and has the features of simple in process, easy operation, work smoothly and complete the drying in one unit

    與傳統的煤炭乾燥系統相比，盤式乾燥機為間接乾燥，乾燥介質與被乾燥物料不直接接觸，避免了污染；運轉穩定，操作簡便；工藝系統簡單，相當于單機完成乾燥作業。
  5. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  6. Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase. the expression of foreign genes in rfpvs were confirmed by ifa. trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge

    純化后脂質體轉染，藍斑篩選純化得到穩定的重組病毒rfpv - ps - ha - pe / l - f ，間接免疫熒光法證實， ha基因和f基因同時得到了表達。
  7. In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression

    本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料，從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究，企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的
  8. Soil - ecosystem has done much with the stability of agriculture, recently the facts influencing the function of soil - ecosystem were not restricting in polluting from physical or chemical sources, the gm technic applying in agriculture not only made the pumping harvest in agriculture, but produced the more serious harm : transgenic bio - pollution

    摘要土壤生態系統的功能是否正常直接關繫到農業系統的穩定，影響土壤生態系統功能的因素已經不是局限在化學物理污染上了，農業轉基因技術的應用，為農業生產帶來新的增長點，為消除傳統的化學物理污染的同時，也給土壤生態系統帶來了更深刻，難以控制的影響因子：轉基因生物污染。
  9. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  10. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  11. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  12. Then the amplified mtb8. 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector

    重組質粒pm 、 pms轉染ps15細胞，進行穩定表達， g418篩選抗性克隆后，用rl 』 - pcr檢測目的基因在mrna水平的表達。
  13. After screening by g418, jurkat cell lines were established to express scfvs stably

    穩定轉染jurkat細胞，經g4篩選，建立了穩定表達scfv融合蛋白的細胞系。
  14. Jurkat cell lines were established to express scfv fusion proteins stably. the products of stably transfect cells and their activities were analysed

    穩定轉染jurkat細胞，建立穩定表達scfv融合蛋白的細胞系，並分析其表達產物的活性。
  15. ( 2 ) after transiently transfected in cos - 7 cells, secreting expression were detect. jurkat cell lines were construct to express scfvs stably. the activities of the products were confirmed

    穩定轉染jurkat細胞，經篩選獲得穩定表達scfv融合蛋白的細胞系，並證實表達產物具有與hbsag特異性結合的功能。
  16. Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999, and its molecular functions were not well understood. it mainly expresses in terminally differentiated cells. by transfection of restin into tumor cell, the cells were arrested in g1 phase and the cell proliferation was inhibited

    Restin是1999年本室在研究全反式維甲酸誘導細胞分化時克隆得到的一個人類未知功能的新基因，該基因主要表達于終末分化細胞，穩定轉染該基因的細胞出現g1期阻滯，抑制細胞的增殖，提示restin與細胞周期的調控有關。
  17. Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis. the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method

    用脈沖電泳將經過同源重組的修飾的yac克隆分離出來通過脂質體轉化植物的原生質體，希望得到在植物細胞中穩定存在的植物人工染色體。
  18. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  19. ( 二 ) using simulative test and onthogonal test to analyze the theories of folded point reactions of chloroamine disinfectant water and the mixed reactions of conveying tubes water by different disinfectant technique. a ) when organic contaminations of original water reach some degree, the reactions of some organic nitrogenous and activated chlorine in original water and the transfoms between organic nitrogenous and ammonia - n will disturb the produce and stability of 1 - chlonoamino. the curve got by the sutra folded point chlorination theory will have greatly changes

    （ 2 ）採用生產性實驗，分析氯胺消毒水的折點反應理論及不同消毒方式的管網水的混合反應，實驗室及生產實驗表明： a ）當原水有機污染達到一定程度，原水中部分有機氮與活性氯的反應以及有機氮與氨氮之間的轉化將干擾一氯胺的生成及穩定，經典折點加氯理論曲線將發生較大變化。
  20. Recently, human elongator complexes have been isolated. the human complex is a fragile six - subunit complex that interacts with rnap ii and has hat activity directed against histone h3 and h4. depletion of elongator from hela nuclear extracts reduces the ability of these extracts to transcribe chromatin templates, providing biochemical support for the proposed role of elongator during transcript elongation in chromatin

    近來人elongator也被分離，它也是個不穩定的六亞基復合物，可與rnap相互作用，具有hat活性，能乙酰化h3和h4 ，在hela細胞核抽提物中去除elongator可降低抽提物對染色質模板的轉錄延伸速率，提供了該復合物在染色質為模板的轉錄延伸中有功能的生化證據，但是上面的信息都來自體外研究。
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