突變菌株 的英文怎麼說
中文拼音 [tūbiànjūnzhū]
突變菌株
英文
mutant strain- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 株 : Ⅰ名詞1. (露在地面上樹木的根和莖) root and stem of a tree above the ground 2. (植株) individual plant; plant Ⅱ量詞(棵)
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
- 菌株 : bacterial strain
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Through a series of mutagen tests, a mutant strain of monascus spp ( m 12 - 69 ) from ml2 was acquired, which can produce much higher monacolin k than m12, and almost same citrinin level as m12
以之為出發菌株,通過誘變篩選,獲得一株monacolink產率有明顯提高而桔黴素產率僅為0 . 13ng g ( elisa )的突變株,編號為m12 - 69 。Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測,發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分,阿維菌素的總產量和b1的產量與出發菌株相當,說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。Antimicrobial agents susceptibility of this strain illustrated that it was resistant to gentamicin, streptomycin, ciprofloxacin, ceftazidime and imipinem
應用此條件經過不斷篩選,最後獲得對多種抗生素耐藥的突變菌株pa9911 - c64 。In most wild type e. coli strains containing heterologous pha synthase genes, the amount of pha accumulation was almost undetectable while in e. coli km32b the amount of pha accumulation were clearly visible
在多種野生型大腸桿菌中,盡管使用了各種不同的表達載體,也很難獲得可供檢測的pha ;但應用大腸桿菌的fqdb缺失突變株卻可以獲得較多量的pha 。We conclude that the gene we studied is a new loci required for efficient nitrogen fixation and for competitive nodulation of soybeans by bradyrhizobium japonicum strain gx201
我們認為在突變體菌株gx217中tn5gusa5所插入的開放閱讀框架是一個新的與競爭結瘤有關的基因。Compared to 042bm, the noea deletion mutant 042bma - km showed different degree of increase in nodule number, weight of nodule nodule and plant top dry weight on cultivars of putong zihua, baoding, ningxia, baifa and aohan, but decrease on milu. however, this mutant has no significant change in ability to nodulate cultivars of huanghou and zahua. hence, noea is involved in cultivar - specific nodulation
與苜蓿中華根瘤菌042bm相比,敲除noea的突變株042bma - km在普通紫花、保定、寧夏、百發和傲漢苜蓿品種上的結瘤數、根瘤鮮重和植株地上部分的干重都有不同程度的增加,而在秘魯苜蓿品種上的結瘤數和植株地上部分的干重明顯下降,在皇后和美國雜花苜蓿品種上則沒有明顯的變化。Breeding of alkaline lipase overproducing strain by screening resistant mutant
抗性突變株篩選法選育堿性脂肪酶高產菌The distribution fractions of mutants with higher avermectin titer than the original had correlation with implantation dose. after ion implantation mutation there were many types of morphology of colony, we stdudied the relationship between the colony shape and the ability of yielding, found that gray and protuberant colony had the highest titer
離子注入后阿維鏈黴菌的菌落形態發生了很大的變化,其中灰色,邊緣整齊,中間突出有開裂的菌落和為灰色,邊緣波浪狀,中間突出有放射狀開裂的菌株產量較高。4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk ( arod : : arobkan ~ r )為了改變代謝途徑脫氫奎尼酸( dhq )分支點上的代謝流量,使之充分流向目的產物奎尼酸合成方向,利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體,使dhq脫水酶( dhqase )失活,阻斷了碳代謝流流向芳香氨基酸生成的方向,同時用同源重組的方法將arob基因定位整合入染色體上,解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響,並減輕代謝負擔。This fact suggested that dndb and dndc were directly involved in the novel dna modification in wild type s. lividans
這兩個突變株均喪失了dnd表型,暗示這些基因與變鉛青鏈黴菌的異常修飾直接相關。In this study, lexa mutant of deinococcus radiodurans xe has been successfully constructed. the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different times of mmc
本研究利用已構建的抗輻射菌lexa基因突變體菌株xe ,經受不同劑量射線照射和不同時間的mmc作用,平板菌落計數,繪制存活曲線。The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現,突變株發酵液中有三種類型的木聚糖酶,而出發菌株中只有兩種類型的木聚糖酶,並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現,突變株中第三種類型的木聚糖酶不僅表達量很大,活力也很高。281 double crossover mutants were examined by fermentation experiments and hplc analysis for production of polyketides. besides avermectin b1a and b2a, a new compound ( about l ~ 4ug / ml ) which showed a retention time and fragmentation patterns identical to those of the authentic sample of 22, 23 - dihydroavermectin bla by hplc and lc / ms analysis were detected in culture extracts of 27 isolates
在篩選的281個雙交換突變株中,有27株除了產生阿維菌素b1a和b2a ,還產生一個新組分,經hplc和lc ms分析,證實該組分為c - 22 , 23雙氫阿維菌素b1a (即伊維菌素b1a ) 。The total amount of avermectins b were only about 0. 5 ~ 2 % of those produced by the parental strain olm73 - 12. this study suggests that t
在281個突變株中,還篩選到了2個突變株,其發酵產物幾乎全部為有效組分阿維菌素b1 , b2組分僅佔5 。After ten generations growing on drug - free plate, the resistance of pa9911 - c64 kept unchanged. 800 broth samples of endophytes were tested on this model, among which several samples showed antimicrobial or synergic activity
以此耐藥突變菌株作為模型,對800份植物內生菌所產生的發酵樣品進行了初步篩選,並獲得了幾份活性樣品。. the conditions of the liquid state and solid state fermentation that m12 - 69 produced monaclin k were studied
第五,對紅麴黴突變菌株m12一69產monacofink的液態和固態發酵條件進行了初步優化研究。After the primary and secondary selection, 7 strains were tested to have higher productivity than the starting strain. of the 7 strains, strains s1 - 007, s1 - 123 and s1 - 018 were proved to produce more maduramicin than the starting strain. compared with 6236ug / ml of the starting strain, the productivity of the above three strains reached 8020 g / ml, 8254 g / mland9175 g / ml respectively
出發菌株尤馬馬杜拉放線菌s - 1 ,經過鏈黴素抗性篩選,挑取223個菌株,經過初篩和復篩得到7株菌株馬杜黴素的產量高於出發菌株,其中3個菌株s1 - 007 、 s1 - 123和s1 - 018的產素量與出發菌株的6236ug ml相比有較大提高,分別為8020ug ml , 8254ug ml和9175ug / ml ,尤其是突變菌株s1 - 018的產量比出發菌株高47 。Lividans mutant strains la1 and la2 generated by gene replacement in dndb and dndc were obtained respectively. both of these two mutants lost dnd phenotype because of disruption of a respective dnd gene
首先利用基因置換實驗獲得分別在dndb 、 dndc基因內部發生基因中斷的突變菌株la1利la2 ,並通過southern雜交進行了驗證。This investigation aims to screen out the high - yield acidic phytase - producing microbial strains and men further to select for high yield strains by mutagenesis and still then to clone and analyze the phytase gene in the high - yield phytase - producing mutant strains. on these bases, to construct high yield phytase gene engineered microbial strains for the industrialized production of phytase. main results are as follows
本研究的目的旨在通過從土壤中篩選酸性植酸酶高產菌株,並進一步通過誘變選育提高其產量,對高產突變菌株植酸酶基因進行克隆和分析,在此基礎上,構建植酸酶高產基因工程菌,從而為植酸酶產品的工業化生產奠定基礎。An auto - controlled fermentor was used to culture these strains under nitrogen fixation conditions and the nitrogneases were effectively depressed
在固氮條件下,所構建固氮酶突變菌株均表現為嚴格的nif ~ -表型,不能進行固氮生長。分享友人