端粒序列 的英文怎麼說

中文拼音 [duānliè]
端粒序列 英文
telomeric sequence tel
  • : Ⅰ名詞1 (東西的頭) end; extremity 2 (事情的開頭) beginning 3 (門類; 方面) item; point 4 (原...
  • : Ⅰ名 (小圓珠形或小碎塊形物) small particles; grain; granule; pellet Ⅱ量詞(用於粒狀物)
  • : Ⅰ動1 (排列) arrange; form a line; line up 2 (安排到某類事物之中) list; enter in a list Ⅱ名詞1...
  • 端粒 : perultimate chromomere
  1. It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking. the rob gene was amplified from macronuclear dna of euplotes octocarinatus. the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes

    本研究利用pcr技術從游仆蟲( euplotesoctocarinatus )大核dna中擴增出rab基因,並對該基因進行分析,該基因全長為783bp ,兩端粒序列,編碼框為624bp ,編碼207個氨基酸,開放讀框中有3個tga ,在此編碼半胱氨酸。
  2. Telomere of yeast is rich in g and a that is ideal target of mms

    酵母富含g和a ,是mms作用的主要靶點。
  3. Other chromosome elements, telomere and ars, have also been cloned for constructing artificial chromosome. arabidopsis telomere was cloned from pcr products using telomere repeat primers without other template. a 2000bp fragment of ars was released from arabidopsis genomic bac clone t14a4 by claidigestion and subcloned into clai digested pbluescript

    擬南芥的是利用的重復進行無模板的pcr擴增得到的;約2000bp的ars片段是從擬南芥的bac克隆t14a4中用限制性內切酶c1a1切下,然後亞克隆到通用載體pbluescript上。
  4. Discussion the ends of linear chromosomes are protected by telomeres that consist of double - stranded repetitive sequences complexed with specific telomere - binding proteins

    討論由雙鏈重復dna及特異的結合蛋白組成,能保護線性染色體的末
  5. Telomerase is at end of the telomere, it helps to keep the sequence of dna, to offset or postpone the continuously shortening of the telomere while the cell division takes place

    酶位於,作用是合成dna,以抵消或延緩隨細胞分裂的不斷縮短。
  6. At the same time, the c - terminal expressing plasmids of hcap - e and hcap - c that attached the kozac sequence and the nuclear localization signal were also constructed

    同時也構建了附加kozac和核定位信號的hcap - e和hcap - c的c -末真核表達質
  7. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n缺失27個氨基酸的淋巴毒素融合蛋白的重組質,在大腸桿菌中實現融合蛋白的可溶及分泌表達,同時利用表達載體上的幾種特殊經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ,為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  8. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp , r _ 1長36bp ,其它片段均40bp長, f _ 1和r _ 1片段兩分別加上限制性內切酶nco和xho的識別位點。用成對單鏈片段進行延伸反應,然後用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質,採用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定,獲得300bp的片段。
  9. Because of its fully sequenced genome and the most complete, contiguous nucleotide sequence of centromeric dna in plants, arabidopsis was chose as the base to construct plant artificial chromosome. we identified two candidate yac clones, cic8h8 and cic6f6, which are both proved to have the 178 bp arabidopsis centromeric repeat sequences

    我們通過pcr和分析,選擇了含有擬南芥著絲區178bp串連重復的yac克隆作為構建人工染色體的基礎,同時也克隆了擬南芥的和ars等
  10. 4. the seguence of cloned endostatin gene was confirmed with the dideoxy chain - termination method and is consistent with reported sequence of mouse endostatin

    4 .以雙脫氧末終止法對重組質pbv220一endostatin進行測,結果表明與己知小鼠endostatin基因一致。
  11. Portion of mitochondrial dna control region form 39 individuals which belong to cxsp and xzsp respectively were sequenced in the study

    來自宣州種群和長興種群的39個個體的線體dna控制區5 』非重復沒有任何差異。
  12. Expression of human telomere repeat binding factor1 in acute leukemia cells and its correlation with telomerase activities

    重復結合因子在急性白血病細胞中的表達及與酶活性的關系
  13. The shrna based on telomerase htert gene 1573 - 1591 sequence can silence hela cell telomerase gene expression in a detectable extent

    酶tert基因1573 ? 1591位的核酸構建的shrna可對hela細胞酶基因表達產生一定的沉寂作用。
  14. Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr

    從pet22 - egf質中分離出末帶his - tag的egf基因,對位融合於多角體蛋白n116個氨基酸基因的下游(命名為ph - egf ) ,並在兩段基因間設計了凝血酶xa蛋白酶切位點,經過酶切、測等鑒定正確后,克隆至pbacpak8中,使ph - egf融合基因置於多角體蛋白( polyhedrin , ph )基因啟動子控制之下,構建成重組轉移載體pbacph - egf 。
  15. Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies, with clear bound. alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony. after the sequential neural induction, the cells converted into neuron - like cells, with homogeneous forms, which had round bright cell bodies and thin long bipolar or multipolar processes

    5誘導分化前後細胞酶活性檢測( trap )結果1 .使用貫誘導法可將escs大比例誘導分化為神經元樣細胞未分化的escs聚集成團狀生長,細胞排緊密,集落邊界清楚,堿性磷酸酶染色呈強陽性,胞漿布滿棕黑色顆
  16. Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters

    設計引物時,上下游引物5 』分別添加了kpn和bamh酶切位點,克隆得到的基因片段和質載體都用kpn和bamh進行雙酶切后,進行酶連、轉化、篩選得到陽性重組子,經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系的驗證后進行測
  17. To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase

    方法:根據酶htert基因1573 ? 1591位的核酸,構建帶t7啟動子的部分雙鏈dna模板,用t7rna聚合酶體外合成短鏈shrna 。
  18. In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a

    通過重新設計引物在ts87兩加上用於構建重組質的酶切位點hind和bamh和用於真核表達的kozak。將改造后的基因片段克隆入t - vector ,轉化大腸桿菌dh5 ,通過藍白斑篩選獲得陽性克隆后測鑒定。
  19. The researchers also collected dna samples from participants, and examined the length of telomeres - repeated sequences at the end of chromosomes in white blood cells ( leukocytes )

    研究者還採集了參與者的dna樣本,並檢查了白血球中染色體末重復的長度。
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