競爭抑制實驗 的英文怎麼說

中文拼音 [jìngzhēngyìzhìshíyàn]
競爭抑制實驗 英文
competitive inhibition experiment
  • 競 : 動詞（競爭; 競賽） compete; contest; vie Ⅱ形容詞[書面語] （強勁） strong; powerful
  • 爭 : Ⅰ動詞1 （力求得到或達到; 爭奪） contend; vie; compete; struggle for; strive 2 （爭執; 爭論） argu...
  • 抑 : Ⅰ動詞（向下按; 壓制） restrain; repress; curb Ⅱ連詞[書面語]1 （表示抉擇） or 2 （表示轉折） but3 ...
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 實 : Ⅰ形容詞1 （內部完全填滿 沒有空隙） solid 2 （真實; 實在） true; real; honest Ⅱ名詞1 （實際; 事實...
  • 驗 : 動詞1. （察看; 查考） examine; check; test 2. （產生預期的效果） prove effective; produce the expected result
  • 競爭 : compete; vie; contend
  • 抑制 : 1 （控制） restrain; control; check; hold up; curb; stop; repress; bridle; choke; prehension; sup...
  1. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  2. Our previous work has also shown that there is a cam - binding site within maize cytoplasmic hsp70 and that hsp70 binds cam in a ca2 + - dependent manner ( sun et al, 2000 ). the high conservation of cam - binding suquence implies that the binding of cam to hsp70 might have an important biological function

    先前我們在玉米中，通過體外結合和競爭抑制實驗證明了熱激蛋白家族中最重要的成員之一純化的細胞質hsp70能與cam結合，並且證明了玉米細胞質hsp70中存在一個非常保守的21個氨基酸組成的cam結合序列。
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