篩別分析 的英文怎麼說
中文拼音 [shāibiéfēnxī]
篩別分析
英文
mechanical gradation-
The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。Firstly, we study the construction of emotion - speech template database, and analyze the common features such as pitch, energy and formant. after choosing the useful features by using fuzzy entropy effectiveness analysis, we get better performance with the application of neural network. in addition, we propose some more efficient features such as speech rate, pitch slope, mel - frequency cepstral coefficients and its transient parameters, and design a processing model based on vector quantization for cepstral features to fusing different features
本文首先介紹了情感語音數據庫的建立情況，然後研究了基音頻率、振幅能量和共振峰等目前常用的情感特徵在語音情感識別中的作用，並且通過一種基於模糊熵的特徵有效性分析方法進行了有效特徵的篩選，應用人工神經網路建立了初步的語音情感識別模型，經過實驗發現特徵篩選后系統的識別效果有著一定程度的提高。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。At the part of empirical study, this paper chooses 124 a - stock listed companies from stock exchange of shanghai and shenzhen as research samples, makes use of the step method to filter the 13 possible variables ( including 3 debt - paying ability indexes, 3 operating ability indexes, 4 profitability indexes and 3 cash flow indexes ) in analysis and finally sets up discrimimant models by fisher discrimimant rules with the samples " financial data. then this paper validates the stability of these models using the self - validation and cross - validation before analyzing the prediction ability of these models with outside - validation
實證研究部分主要以我國深、滬兩市124家上市公司為研究樣本，採用上市公司1998年至2001年的實際財務數據，運用逐步判別分析方法對擬進入模型的13個變量（包括3個償債能力指標、 3個經營能力指標、 4個獲利能力指標及3個現金流量指標）進行篩選，並根據fisher判別準則建立判別模型，在運用自身驗證法與互動式驗證法對模型的穩定性加以驗證后，運用外部數據驗證法對模型的預測能力進行了分析。At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。Secondly, introducing the image analyzing technology with reference to the disadvantages of the traditional ferr - graph analysis technology, and with the combination of characteristic parameter optimizing filtration so as to raise a description method of debris micro - morphologic character. thirdly, with the application of mode recognition method, completing the process of debris auto - recognition based on the collected information of the debris configuration characteristics ; and conducting the diagnosis on the aero - engine wear faults according to the theory of particle tribology. fourthly, introducing information fusion technology to solve the problem that a single method can not collect enough fault premonitory information to conduct the wear fault diagnosis, hence to conduct the research and exploration in the field of comprehensive diagnosis on the aero - engine ' s multi - fault premonitory information
本文的研究工作主要包括以下五個部分：首先，介紹航空發動機常見的磨損故障類型，研究磨損故障的失效機理，分析磨粒的產生機理、分類以及形態特徵：其次，針對傳統鐵譜分析技術的缺點，引入圖像分析技術，再結合特徵參數優化篩選，形成基於圖像的磨粒顯微形態學特徵描述方法：然後，基於提取到的磨粒形態特徵信息，應用模式識別方法完成磨粒自動識別，並根據顆粒摩擦學的基本原理進行航空發動機磨損故障的診斷與定位：再后，鑒于單一方法不能提取足夠的故障徵兆信息進行磨損故障診斷，本文引入信息融合技術，開展航空發動機多故障徵兆信息綜合診斷方法的研究與探索；最後，基於航空發動機滑油光譜分析與鐵譜分析數據，應用時序模型、灰色模型以及組合模型進行磨損故障的預測方法研究。As we know, the key of polynomial selection in nfs are size and root properties. in this thesis we deeply discuss and study these two factors
本文分別對影響數域篩法中多項式選擇的兩大因素：多項式大小和根的屬性進行了深入的分析和研究。According to the special shape and operate requirements of the torpedo, the structure and moulding process of the torpedo shell are established, in which the body and overlay block with grooves are moulded by fiber winding, and close moulding respectively with the adhesive by limited stop compression. in the light of the selection of raw material system, composite system with outstanding performance are defined, based on which the mechanical properties are tested., with the overall consideration of structural characters and design demands, the main factors which may have effects on the strength and stability ( including stiffness section dimension, stiffeners space, skin thickness and skin lay angle ) is optimized one by one for the structure design parameters on the base of the fem model of skin, stiffness and layover block by fem. as a result, the prototype with 1 : 1 proportion and its structure and lay optimization design are completed. the moulding technology for polyurethane foam moulding the stiffened shell is obtai ned in terms of the design, manufacture moulding, experimental mould and tooling
依據在研產品特殊的外型結構和使用要求，確定了該型號水雷復合材料殼體的具體結構形式和復合成型工藝路線，即由纖維纏繞工藝成型主體異形件，閉模成型帶通槽外貼塊，用限位加壓工裝實現粘接的工藝路線；通過對原材料體系的篩選，確定了綜合性能較好的復合材料體系，以此為基礎進行了力學性能測試；運用有限元方法，建立了蒙皮、筋條和外貼塊的分析模型，綜合考慮結構特點和設計要求，對影響結構強度與穩定性的主要因素（包括筋截面尺寸、筋間距、蒙皮厚度、蒙皮鋪層角度）分別進行了結構設計參數的優選，最終完成了復合材料異形耐外壓殼體1 : 1樣件和縮比實驗件結構及鋪層優化設計；完成了聚氨酯泡沫胎成型內置加筋殼體的成型工藝技術研究；對縮比件進行外壓性能測試，並給出了應力、應變測試結果。Among 54 arbitrary primers tested, only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae
本研究經用54種隨機引物對57種蟋蟀的rapd擴增分析得出以下結論： 1 ）採用rapd技術，首次較系統地對中國蟋蟀總科7科26屬共57種蟋蟀進行了系統學研究。在所試用的54種隨機引物中篩選出可擴增出清晰可辨且穩定的rapd圖譜的引物分別為：蟋蟀科9種、嶺蟋科9種、樹蟋科12種、結蟋科12種、蛛蟋科13種、鐵蟋科13種、貌蟋科13種。Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。Coli have been performed in this study. engineering bacteria, screened for efficient expression of pheromone 3, express the proteins in type of insoluble ( inclusion ) and soluble forms, respectively
本實驗對游仆蟲信息素在大腸桿菌中的表達進行了系統的比較與分析，篩選獲得了有效表達信息素的工程菌株，表達產物分別為包涵體與可溶性蛋白。A series methods of data combination analyzing are selected to form the operating method system for crop discrimination. combining gis, gps, and other data from field work with rs data can determine interpretation features and set off working regions, combining rs data can enhance spatial features in order to do unsupervised classification efficiently, union of gis data enable us to join maps and extract features, to analyze crop structure, crop calendar, cultivating system
本項研究篩選出了構成運行化作物遙感識別技術體系的一系列數據復合分析方法，包括gps 、 gis數據以及其它田間作業信息與rs數據之間的復合，確立解譯標志和劃分作業區； rs數據之間的復合，進行圖像增強，改善非監督分類效果； gis數據之間的復合，分析作業區作物結構、物候和耕作制度現狀，地圖拼接、特徵提取等。2 making use of parsing of xml, system can analyze, operate and research the content in xml bidding document automatically, it do preprocess the bidding choice before choosing through public appraisal, which function adds intelligence in online government store system
利用xml可解析性，在評審標書時除了人工評審外，系統可自動對投標書的xml文件內容進行分析、運算重組以及檢索，從而能夠進行智能的判別，篩選理想的候選； 3This paper uses quantitative evaluation method and principles of diagenetic reservoir facies, gray theory, matches and picks up petrophysical parameters, analyzes the comprehensive effect of evaluation parameters on diagenesis, establishes parameter index and automatic analysis and processing method for diagenetic reservoir facies evaluation with gray theory, integrates gray system theory and multi - information, and provides an important method and basis for reservoir description, heterogeneity study, oil well productivity analysis and screening favorable oil blocks
摘要根據成巖儲集相定量評價分析方法和準則，利用灰色理論思維和手段，匹配、擬合和提取儲層物性參數，分別以評價參數對成巖作用的綜合效應進行分析，建立起灰色理論成巖儲集相綜合評價參數指標和自動分析處理方法，實現了灰色系統理論集成和多種信息綜合，為油藏描述、儲層非均質性研究、油井產能分析和含油有利區塊篩選提供了重要方法和依據。By analyzing the rainfall data of 20 hydrological stations in the miyun reservoir basin from 1970 to 1993, the relationship between 45 heavy rainfall events and synoptic situations, nwf outputs, the forecast indexes and synoptic patterns are put forward, and 24 - hour heavy rain forecast equations of june, july, august in the miyun reservoir basin are developed
通過整理1970 - 1993年24年間水庫流域內20個水文站雨量資料，分析45個暴雨天氣樣本與歷史天氣形勢和數值預報產品的關系，篩選出預報指標和預報因子，使用數值預報產品的解釋應用方法，根據天氣環流形勢的分型，分別組建了6 、 7 、 8月每個月份的未來24小時暴雨天氣預報方程。First of all we discuss the model of information purifying and bring forward the methods of setting up the according fuzzy set and subject function. secondly after analyzing the traditional technology and the strongpoint and the shortcoing of information purifying we improve it combining with the technique of fuzzy mode identifying, data warehouse, cache etc. and we can perpetrate an on - line and synchronous purifying through analyzing the text and picture showing in the pages of network. finally, we choose sql server 2000 to design the url database and delphi, wingate as the tool for system development to develop an efficient system of information purifying which can keep the network consumer especially young student apart from the intrusion of unfriendly information and make the environment of network pure and fine
本文首先探討了該系統中的信息「凈化」模型，提出了模型中的模糊集及隸屬函數的構造方法；然後分析了傳統的信息「凈化」技術及其優缺點，結合模糊模式識別、數據倉庫、高速緩存等技術對傳統的信息「凈化」技術進行了改進，改進后的信息「凈化」技術可通過分析正在顯示中的網頁文字、圖片內容，做即時、同步性的網頁內容篩選；最後，利用sqlserver2000設計了url數據庫，選擇delphi 、 wingate作為系統開發工摘要具，設計開發了一種高效的網路「凈化」器，使網路用戶尤其是青少年學生遠離非友善信息的侵擾，讓網路環境更加純凈、美好。To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity, the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast, respectively
經過兩輪抗生素篩選后，採用fmdvvp1基因的一對引物和煙草葉綠體同源片段的一對引物分別對抗性植株的葉綠體dna進行的pcr和pcr - southernblot分析。Meanwhile, the cloning and sequencing of which was also performed. and the main results were as follows : ( 1 ) only two obvious polymorphism bands were obtained in the fertile line from selective pcr amplification with 64 pairs e + 3 / m + rimer combinations, and which were named as mf - 14 and mf - 24, respectively. moreover the polymorphism band mf - 14 was cloned successfully
結果如下： （ 1 ）本實驗分別以可育系和不育系基因組dna混合池為模板，選用64對e + 3 m3引物組合進行選擴篩選，分析得到了兩條可育系所特有的差異條帶mf - 14和mf - 24 。Extended approach three : a hierarchical reduction approach is imported, which is based on the combination of statistical feature selection and linear discriminant analysis
拓展演算法三：引入統計篩選和線性判別分析相結合的分層遞階約簡演算法。分享友人