篩后子 的英文怎麼說

中文拼音 [shāihòuzi]
篩后子 英文
rima ethmoidal posterius
  • : 名詞[書面語] (植物名) sedge
  • : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化經分量比較、 pcr鑒定和酶切分析選陽性克隆。
  2. Add in the sifted flour, cinnamon, baking powder and hazelnut powder into the butter mixture. then add in chocolate drops and mix

    加入過的麵粉,肉桂粉,烤粉和榛粉拌勻即可。最後加入巧克力拌勻。
  3. Molecular sieve dryer, air dew point is - 40 after drying

    乾燥乾燥空氣露點達到- 40
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因形成一終止密碼,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
  5. 1. a new method to identify _ amylase activity and its producing bacteria : the blue complex was formed by unspecific adsorption, after mixing starch and trypan _ blue. the adsorption weakened when the starch was hydrolyzed to small molecular by _ amylase, and the trypan _ blue was released inside the hydrolyed zone. the starch around the zone which was not hydrolyzed adsorbed free trypan - blue so that the colour of medium became bluer than that of place in hydrolyzed zone

    快速鑒定並選-澱粉酶及其產生菌的新方法:錐蟲藍染料和澱粉由於靜電非特異性吸附結合使澱粉呈穩定的藍色,當澱粉被澱粉酶水解因分變小吸附力減弱,而讓錐蟲藍游離出來,游離的錐蟲藍被周圍未水解的澱粉吸附而使顏色加深,澱粉水解區則形成無色、透明的水解圈。
  6. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種,對其進行了分生物學的驗證及生理指標的檢驗。
  7. The results are obtained as follows : we analyze the features of kinematics and dynamic of the crusher. the improvement on the crusher structure design is made in the following aspects : involutes beat board, adjust mechanism on the back protect board and sieve plate, distributaries system, sieve plat, crusher space, rotor, hammer. furthermore, strength check is carried out

    通過本題研究取得了以下研究成果:對破碎機進行了運動和動力學分析,結構優化改進,包括:漸開線反擊板設計,側護板和板的恆力調節機構設計;分流系統的設計;板的設計;破碎腔的設計;轉的設計;錘頭的設計。
  8. In order to study the influence factors of aoa of rose flowers, the effects of drying and extraction methods on the aoa of rose flowers were investigated. the results indicated that drying after high - temperature short - time pretreatment was rather effective to maintain their aoa ; the aoa of water extracts was stronger when the temperature was raised from 25 to 100 ; by using orthogonal test, the optimum extraction conditions of rose flowers were : solvent - 75 % ethanol ; ratio of material and solven - 1 : 10 ; extraction times - three times with 24 h at one time, at the room temperature. the extracts obtained by 75 % ethanol were fractionatedly extracted with petroleum, diethyl ether, ethyl acetate and n - butanol in turn, and the various fractions " aoa were analyzed

    為了探討玫瑰花抗氧化活性的影響因,比較了不同乾燥方法、提取方法對其抗氧化活性的影響,發現:經短時高溫處理再進行乾燥有利於較好地保持玫瑰花的抗氧化活性;以水作溶劑提取時, 25 100范圍內水提液的抗氧化活性隨著溫度的升高而增強;通過正交實驗選得到常溫下玫瑰花抗氧化活性物質的最佳提取方法為: 75乙醇為溶劑,液料比1 : 10 ,提取3次,每次24h ; 75乙醇提取物依次用石油醚、乙醚、乙酸乙酯、正丁醇等有機溶劑進行兩相分部萃取,發現玫瑰花的抗氧化活性物質主要存在於乙酸乙酯部,說明玫瑰花抗氧化活性主要成分可能是單寧類、黃酮苷類和原花色素類化合物; 4
  9. Investigations also show that the catalyst shows good heat resistance in the presence of water and demonstrates high capability for mitigating sox poisoning. in the end, a novel way controlling combustion is employed in a 1. 342l s. i. engine, in which lean - burn, fast burn ( tumble ) and delay ignition make the engine provide much cleaner exhaust gas than that of a conventional premixed s. i. engine

    最後綜合採用機內凈化和排氣處理并行策略,即稀燃、快燃(滾流) 、推遲點火的可控燃燒新方案,結合分稀燃催化器,在稀燃發動機典型工況下,在保證燃油經濟性較原機改善14 . 8 %時, nox的排放大幅降低。
  10. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化,轉化酵母宿主菌gs115原生質體選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離交換, c _ ( 18 )反相hplc純化得到分量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。
  11. Principle of operationthe ring hammer granular crusher makes use of impact hitting hammer rings cross mounted on the rotating rotor to crush materiel. after first crushed by impact force of hammer rings, the materiel is then pressed, sheared and ground between crushing plate and ringhammers to the required granular product which flows off the screen, and those uncrushed such s tramp iron, wood pieces, stone etc will be brought into the tramp iron trap. this crusher features little blast wind, less vibration andlong, service life of vulnerable

    工作原理環錘式破碎機利用旋轉的轉帶動環錘對物料進行沖擊破碎,被沖擊的物料又在環錘和破碎板、板之間受到壓縮、剪切、碾磨作用,使物料達到所需的粒度,被破碎的物料經板的柵孔落下,不能被破碎的鐵塊、木塊、石塊等經撥料板撥進除鐵室.本機具有鼓風量小、振動值小,環錘等易損件壽命長等特點。
  12. Results of the dsc analysis show that the addition of mcm - 41 do increase the rate of crystallization of pet. the crystallinity of pet / mcm - 41 nanocomposite is much higher compared with other fillers

    實驗發現,介孔分mcm對烈5o的填加量與pet共混可明顯地增加pet的結晶速度。
  13. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達:分別將csfv兩個代表株的e2基因克隆入畢赤酵母( p . pastoris )分泌型表達載體ppic9k中,酶切線型化電穿孔導入p . pastotis進行整合,經g418選得到25個高拷貝轉化,經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  14. In autograft from inflorescence stems callus tissue was found 4 days after grafting. no xylem and sieve elements were differentiated in both scion and stock in this period

    C24擬南芥花序軸自體嫁接4d ,嫁接面處愈傷組織開始形成,嫁接體雙方還沒有分化出管狀分
  15. In autograft of arabidopsis thaliana ( columbia24 ecotype ) grafted in the position of hypocotyls, wound xylem and sieve elements were differentiated in the tissues both of scion and stock 4 days after grafting

    C24擬南芥下胚軸自體嫁接4d ,在接穗和砧木中就已有管狀分的分化。
  16. The researchers say their analysis of cancer screening tests from holland supports the theory that sunlight suppresses women ' s immune defenses, so they are more likely to get a virus that causes cervical cancer

    研究者說,通過分析荷蘭癌癥選實驗,發現日光會抑制女性免疫防禦機能,使她們容易感染能引發宮頸癌的病毒。
  17. By molecular design, high shrinkage polyamide for fiber was synthesized. the chip after spinning and drawing has a shrinkage of 20 ? 40 % in boiling water. the spinning speed is above 4500 m / min

    藉由分設計,選共單體,合成高收縮尼龍粒,紡速可達4500米以上,纖維經延伸,沸水收縮率20 ? 40 % 。將此纖維透過織物設計製成紡織品,具超蓬鬆感或緻密感,並有特殊光澤。
  18. By molecular design, high shrinkage polyamide for fiber was synthesized. the chip after spinning and drawing has a shrinkage of 20 40 % in boiling water. the spinning speed is above 4500 m / min

    藉由分設計,選共單體,合成高收縮尼龍粒,紡速可達4500米以上,纖維經延伸,沸水收縮率20 40 % 。將此纖維透過織物設計製成紡織品,具超蓬鬆感或緻密感,並有特殊光澤。
  19. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基選重組,再利用g418抗性選出整合有多拷貝外源基因的重組
  20. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切,酶切產物純化利用大腸桿菌t _ 4dna連接酶連接構成重組pezz18 - as ,並轉化e . colidh5 ,經氨芐青霉素lb平板初,以菌液pcr和重組的單、雙酶切行進一步鑒定。
分享友人