篩狀變性 的英文怎麼說
中文拼音 [shāizhuàngbiànxìng]
篩狀變性
英文
cribriform degeneration-
Fifty nine accessions of soybean [ glycine max ( l. ) merr. ] of 301 ones from huanghuaihai and middle - lower changjiang valleys were tested at seedling stage in two years for their drought tolerance by using the mean membership index value averaged over those of plant height, leaf number, root dry weight, stem and leaf dry weight. 4 tolerant accessions ( rank 1 ) and 2 sensitive ones ( rank 5 ) were identified
摘要從301份大豆品種中按根系類型選取黃淮海和長江中下游地區代表性材料59份,在苗期乾旱脅迫和非脅迫條件下對地上部和地下部性狀進行2年重復鑒定,發現材料間性狀隸屬函數值具有豐富遺傳變異,以株高、葉齡、根干重和莖葉干重隸屬函數的算術平均數為抗旱綜合指標,從中篩選出漢中八月黃、晉豆14 、科豐1號、圓黑豆等強耐旱型( 1級)材料和臨河大粉青、寧海晚黃豆等乾旱敏感型( 5級)材料。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。This paper summarized the achievement of types, mechanism, method of selection and identification of plant somaclonal variation, and discussed the actuality and prospects of somaclonal variation application in breeding new varieties
摘要本文綜述了有關植物體細胞無性系變異的類型、機理、篩選與鑒定方法等方面的研究成果,並對其在新品種培育中的應用現狀和前景進行了討論。
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