簡並引物 的英文怎麼說

中文拼音 [jiǎnbàngyǐnwù]
簡並引物 英文
degenerate primer
  • 簡 : Ⅰ形容詞（簡單） simple; simplified; brief Ⅱ動詞1 （使簡單; 簡化） simplify 2 [書面語] （選擇人才...
  • 並 : 併名詞1. （山西太原的別稱） another name for taiyuan （in shanxi province）2. （姓氏） a surname
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  1. Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene

    基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。
  2. Automated programming of degenerate primers and the cloning of the diamondback esterase gene

    程序化設計簡並引物與克隆小菜蛾酯酶基因
  3. The traditional homogene cloning method was modified on two aspects : first, homo - primers was designed at the sites with minimum degeneracy instead of maximum homology, secondly, some " n " bases were replaced with inosine in degenerate codes, resulting hi the degeneracy reduced from over 1000 times to below 100 times

    改進后的同源引物簡並度從1000倍以上降低至100倍以下，利用rt一pcr成功地擴增出一條630bp的鹽藻cdna片段，該片段與其它物種的烯醇酶基因具有很高的相似性。
  4. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。
  5. At the point of analyzing the coal transport market, transport means, and the main coal ports, the author specified the market competition which qinhuangdao port faced. based on the whole descriptions of coal transport demands and consumes, we introduced the model of goods distribution and made some analysis about some main coal ports. and through analyzing those aspects we got a whole evaluation of the qinhuangdao port and brought out some realizable measures to the future development, such as : applying the theory of market subdividing, keeping the market share and dealing the consignees " join and developing the straight transport, setting up the center of coal gathering and distributing and dealing, intensifying the management of company, improving the port synthetical ability, fasting the construction of port basic facilities and work the coal transport well

    作者以分析煤炭運輸市場、運輸方式和主要的煤炭運輸港口為著眼點，具體分析了秦皇島港所面臨的市場競爭形勢；通過對煤炭產地和消費地分佈的描述，介紹了煤炭運輸需求和消費的總體概況；並引入物資調運模型對主要港口之間的煤炭運量分配進行了簡單的定量分析；綜合以上分析，對秦皇島港的現狀作出整體評價，提出了切實可行的未來發展對策，即：應用市場細分理論；保住市場分額，做好貨主銜接，發展直達運輸；建立煤炭集散交易中心；強化企業管理，提高港口綜合能力；加快港口基礎設施建設，搞好煤炭運輸生產經營等。
  6. Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae. six novel rip gene fragments ( 408 ' bp ), 1 from benincasa hispida and 5 from cucurbit a moschaia

    根據葫蘆科rip上、下游兩段高度保守的氨基酸序列設計簡並引物對ly1 ly2 ，對基因組dna進行pcr擴增，首次建立了rip新基因快速篩選體系。
  7. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  8. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，並在序列保守區域設計簡並引物，然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 ， 17 - hsd3的中間片段。
  9. To elucidate further the presense and function of integrin - like protein, we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits

    而要進一步從分子水平上證實類整合素的存在以及研究它的功能，必須克隆其基因。根據動物整合素、亞基的胞質域保守序列設計簡並引物，利用3 race的方法擴增、亞基的3末端序列。
  10. Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained

    再次，根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物，與atp結合蛋白的上游簡並引物組合，經pcr擴增獲得2 . 1kb的條帶，測序后通過blast比較，結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。
  11. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  12. The krupple type zinc finger motif found in many transcription factors is thought to be important for nucleic acid binding and / or dimerization. here we have identified and characterized two novel zinc finger genes named znf323 and znf360 using degenerate primers from an early human embryo heart cdna library

    本文利用kr ppel型（ cys _ 2 his _ 2型）鋅指基因順序上的保守特徵，設計簡並引物從人類早期胚胎cdna文庫中初步篩選克隆出四個kr pple型鋅指蛋白新基因，經國際基因命名委員會批準命名為znf323 、 znf360 、 znf359和zfp28 。
  13. Detection of lily mottle virus by rt - pcr using special primers and degenerate primers

    應用特異引物和簡並引物檢測百合斑駁病毒
  14. Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes. a cdna fragment of 208bp was amplified by rt - pcr, which was subsequently cloned into pmd18 - t vector for sequencing analysis

    利用genbank中已經登錄的其它植物中該酶基因的保守序列，設計一對簡並引物，採用rt - pcr技術，從茶樹擴增出208bp的cdna片段。
  15. These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells. two degenerate oligoxynucleotide primers ( c8 - 2, c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8

    根據嗜鹽菌素cs的n端氨基酸序列設計了一對簡並引物（ cs一2 ， cs礴） ，利用這對引物從as7002的總dna中擴增出halcs的部分序列。
  16. Three special bands as pcr products, obtained by using primer no. l, are reclaimed, and then used to cloning and sequencing. through homologous comparison with data from international nucleotide databank, the band ' s functions have been simply analyzed

    對1號簡並引物擴增出的3條特異性條帶進行了回收、克隆和測序；與國際核苷酸數據庫進行了同源性比較，對特異性片段的功能進行初步的分析和確定。
  17. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  18. In this dissertation, the following experiments are conducted : firstly, cloning of hpab - gene, i ) the amino acids sequences of 23 animal - peptide antibiotics were analyzed by multiple sequences alignment method and a pair of degenerated primers were designed according to the consensus sequence derived from mature peptides of some beta peptide antibiotics

    人源肽抗生素hpab -的基因克隆：對動物23種型肽抗生素的氨基酸序列進行對比，選擇部分相似性較高的序列，分析其成熟肽的結構特點，抽提型肽抗生素的一級結構特徵，並以此為基礎設計簡並引物。
  19. Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl

    應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。
  20. In order to study mhc genes of chinese a igator ( alligator sinensis ), a pair of degenerate primers were used to amplify the segments of mhc class ii b genes from the genomic dna of chinese alligator. ten 166 bp ( one 160bp ) - long different nucleotide sequences, which were divided into two groups ( a, b ), were obtained from cloning and sequencing

    為了研究揚子鱷（ alligatorsinensis ）的mhc基因，本文利用一對簡並引物擴增出揚子鱷mhc類b基因第二外元的部分片段，並對其進行了克隆及序列分析，結果得出10種不同序列，片段長166bp （其中一種為160bp ） 。
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