粒子電泳 的英文怎麼說
中文拼音 [lìzidiànyǒng]
粒子電泳
英文
particle electrophoresis-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Keywords : sedimentation velocity and potential, electrophoretic mobility, electric conductivity, charged composite particle, charged porous particle, arbitrary double - layer thickne
關鍵詞：沉降速度與電位、電泳可動度、電導度、帶電復合粒子、帶電多孔性粒子、任意電雙層厚度。Using diethanolamine as aminating agent and glacial acetic acid as neutralizing agent, aminated epoxy acrylic cationic resin was prepared. the effect of technology of aminated epoxy acrylic resin on properties of eletrodeposition was studied by conductivity meter and electrophoresis apparatus. it was shown that, conductivity firstly decreased, and then increased with aminating temperature increase. in contrast with putting polyacrylic resin into thin acetic acid solution, the more compact film could be achieved by neutralizing polyacylic resin with glacial acetic acid and then add it into water. when neutralizing temperature was enhanced, the speed of electrodepsidon was found to increase, and the film was also more compact. increasing the dn leads to enhanced conductivity and smaller particle size. when dn equaled to 80, the smoothest film could be achieved
以二乙醇胺為胺化劑、冰醋酸為中和劑，合成了胺化環氧丙烯酸陽離子樹脂.採用電泳儀和電導率儀，研究了胺化環氧丙烯酸樹脂合成工藝對陰極電泳塗料電沉積性的影響.結果表明，隨著胺化溫度的增加，電泳液電導率先下降後上升.將冰醋酸加入樹脂中中和，後用水稀釋，比樹脂在醋酸稀溶液中中和，電沉積性能更好.電沉積速率隨著中和溫度的上升而增加，電沉積膜緻密性相應增加.中和度（ dn ）愈高，電泳液電導率愈大，粒徑越小，而塗膜外觀在中和度為80時達到最佳After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ，其啟動子為lacz ，用iptg誘導， sds - page凝膠電泳檢測lexa蛋白的表達。The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5
將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。Electric field is able to drive the charged particles directional movement along the interlayer of graphite
電泳插層利用電場力的作用，使得帶電粒子沿石墨粒子的層間方向作定向運動。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field
電泳分離法分離物質，尤指分離蛋白質的方法，以及基於在電場作用下處于膠粒懸浮狀態的各個成分的運動速度基礎上進行的分子結構分析方法Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。3. dna double strand breaks ( dsbs ) induced by ion beams of gel electrophoresis experiment has shown positive linear with dose, i. e., double strand breaks will increase as the dose increased
分子生物學技術? ?電泳技術分析結果表明，離子輻射造成的dna雙鏈斷裂（ dsb ）與粒子劑量呈線性正相關關系，即隨著粒子劑量值增大， dsb量也逐漸上升。Status and development of preparation and surface modification methods for electrophoretic particle used in electronic ink displays
電子墨水顯示器用電泳顆粒制備和表面改性的研究進展Puried and identified by gel electrophoresis, the plasmid pbl29 is cccdna and the molecular weight of the plasmid pbl29 was estimated to be about 3. 7kb. the sensitivity of bacillus iicheniformis29 to 18 antibiotics was determined by inhibitory zone with filter paper
Licheniformis29菌株中抽提得到質粒pbl29 ，純化后通過瓊脂糖凝膠電泳觀察，確證質粒pbl29為閉合環狀質粒、分子量3 . 7kb左右。Electrophoresis the migration of electrically charged particles toward oppositely charged electrodes in solution under an electric field - the positive particles to the cathode and negative particles to the anode
電泳：利用溶液中的帶電粒子在電場中朝相反電極方向移動? ?正離子向陰極移動，負離子向陽極移動將溶液中各分子分離的技術。分享友人