粒菌素 的英文怎麼說

中文拼音 [lìjūnsù]
粒菌素 英文
granaticin
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  3. Company owns ten gmp authenticated production lines, which can manufacture various high standard pharmaceutical preparation and crude drug, such as ampoule, freeze - dried acanthopanax powder spasmolytic, injection, ii cephaloridine, granules, troche, capsule, soft capsule, eye drops

    公司擁有十條通過gmp認證的生產線，可以高標準生產包括針劑、凍乾粉針、注射劑、頭孢菌素類、顆粒劑、片劑、膠囊劑、軟膠囊劑、滴眼劑等在內的各種劑型和原料藥。
  4. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  5. These pathogenic characteristics that could cause corm tissue to bring browning reaction, lignose of cell wall to increase, and faecula granule to decrease, were also observed by using tissue sectioning

    用組織切片法觀察發現病菌能使香蕉苗球莖組織產生褐變，引起細胞壁木質素增加及澱粉顆粒減少。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  8. Thus, it is postulated that in the presence of mild neutropenia, whose function is inhibited by effect of insulin excess, the bacillus was able to find a port of entry, probably via micro - abrasions of the bowel mucosal lining

    因此，推測可能存在中性粒細胞減少，功能被過多的胰島素抑制，芽孢桿菌可以乘虛而入，很有可能通過腸內壁粘膜微破損進入循環。
  9. Effect of interleukin - 15 on microbicidal activity of human peripheral blood polymorphonuclear cells against candida albicans

    白介素15對人外周血嗜中性粒細胞抗白念珠菌活性的影響
  10. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  11. The results indicated that the department had complied with the requirements of four key elements indicating the quality of indoor air as recommended by the environmental protection department, namely the intensity of carbon dioxide, air - borne dust particulate, radon and total viable count

    本局委託了機電工程署就本局位於稅務大樓的辦公室進行室內空氣質素測量，結果顯示大樓的室內空氣質素，符合環境保護署建議的四個空氣質素主要成分的規定。這四種含量分別是二氧化碳、空氣微塵粒子、氡和總活菌素。
  12. Preparation of avermectin granular coated with polymer diatomite composites and its properties

    硅藻土為控釋材料的阿維菌素顆粒劑的制備及其性能研究
  13. A conceptual approach including measurements of materials at rest ( step 1 ), measurements using a large rotating drum ( step 2 ) or a particle - flec ( step 2 ) and measurements at a workplace ( step 4 ) has been used to characterize the release of microbial components ( bacteria, fungi, actinomycetes, endotoxin or enzymes ) and particles from straw, wood chips or fungal cultures of different ages on gypsum boards

    一套整體概念性的方法，包括物質在靜止時（步驟一） 、使用大轉動滾筒時（步驟二）或微粒逸散完時（步驟二） ，和工作場所（步驟四）進行量測，以描述由麥稈、木頭碎片或不同年份的石膏板上的真菌菌落所釋放的微生物組成（細菌、真菌、放線菌、內毒素或酵素）和微粒特性。
  14. The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas

    對出發菌株ybt - 1463和其無質粒突變株bmb171的部分形態、生理生化和遺傳學特性進行的比較研究的結果表明，突變株bmb171不形成伴胞晶體，但在個體形態與菌落特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發菌株ybt - 1463無明顯差異。
  15. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  16. 16 replacement plasmids for two different regions were obtained, but have n ' t been introduced into 10 - 22 successfully. 42 cosmids in this region were introduced into a heterogeneous host zx64, one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton

    將300kb區域的柯斯質粒進行異源表達，發現有一文庫質粒5h4的轉化子在硫鏈絲菌素存在時表現出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。
  17. Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

    將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。
  18. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  19. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  20. With high concentration of sds and elevated temprature （ 43 ） ， we succeeded in eliminating plasmids from ten strains of s. aureus which were isolated from the farms of beijing suburb. according to the sensitivity test before and after plasmid remoral ， we found that 72. 5 antibiotic resistance of s. aureus was mediated by plasmids ， and 27. 5 encoded by chromosome. the curing test indicated that the plasmids play an important role in coding the resistance to antibiotics

    從北京郊區雞場分離到10株耐藥性金黃色葡萄球菌，對這些菌株進行質粒消除，根據質粒消除前後的耐藥性檢測，發現耐各種藥物的抗性基因大多數位於質粒上，少部分抗性基因位於染色體上，由質粒介導的耐藥基因佔72 . 5 % ，由染色體編碼的抗性基因佔27 . 5 % ，說明質粒在決定對抗生素的抵抗性中起主要作用。
分享友人
分享到 Line

分享到臉書