Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities
因此將這些蛋白包被、固定到材料表面,觀察骨組織工程種子細胞mscs細胞的
粘附、生長特性是本研究的中心環節,並從以下方面進行探討: ( 1 )採用不同原代細胞分離方法,研究其對mscs細胞的生物學特性影響。 ( 2 )檢測基因勝
肽膠對mscs細胞
粘附、增殖及分化的影響。 ( 3 )分別採用型膠原及纖維
粘連蛋白( fibronectin , fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycolide ) , plga )膜及多孔塊型plga材料,觀察細胞在單層或三維培養狀態下,型膠原及fn對mscs細胞
粘附、增殖及向成骨細胞分化效應及能力。
Objective : laminin 5 is a hetertrimeric glycoprotein with a strong adhesive function consisting of three polypeptide chains ( 03, f ^ y 2 ) that is secreted by keratinocytes
目的: ln - 5是一個由三條多
肽鏈( 3 、 3 、 2 )組成的異三聚體的糖蛋白,由角質形成細胞產生的,具有強大
粘附功能。
The two fragments were ligated to a whole fusion peptide gene, next was ligated with two adapters which have the same cohesive end ( atg / tac ), respectively
首先將其連接成融合
肽的完整基因,然後分別與含有相同
粘性末端( atg / tac )的ecor前接頭和sal後接頭連接。
It was recognized as a brain - gut peptide since the peptide and its receptor exist in the central nervous system. previously, we demonstrated that motilin and motilides activates cells in paraventricular nuclei ( pvn ) reflected by increased gastric motility and induction of the immediate early gene c - fos in conscious rat, which suggests central motilin participates in the regulation of gastric motility. it was reported in 2001 that ghrelin is an appetite - stimu latory signal from stomach with structural resemblance to motilin
胃動素( motilin , mt )是由小腸上段
粘膜內分泌細胞合成並分泌的22個氨基酸組成的腦腸
肽,調節消化間期胃運動, pvn內給予胃動素和胃動內酯( motilides )可增強清醒大鼠胃運動,中樞微量注射胃動素可誘導pvn中立即早期基因c - fos的表達,提示中樞胃動素參與胃運動的調控,人ghrelin和胃動素之間有36的相似性,而且二者的受體之間有50的相似性,因而有人稱ghrelin為「胃動素相關
肽」 。
The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes
兩親共聚物-氨基酸( rgd )雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸( pla )鏈段和親水聚氧乙烯( peo )鏈段的兩親嵌段共聚物材料( pla - peo ) ,利用peo鏈端的活性官能團羥基固定了促細胞
粘附的氨基酸及整合素配體多
肽片段rgd 。
The therapeutic effect and mechanism of octreotide on gastric mucosa esion complicated by acute hepatic lesion
奧曲肽對實驗性急性肝損傷並發胃粘膜病變的治療效應及機制