精液解凍 的英文怎麼說
中文拼音 [jīngyèjiědòng]
精液解凍
英文
thawing of semen- 精 : Ⅰ形容詞1 (經過提煉或挑選的) refined; picked; choice 2 (完美;最好) perfect; excellent 3 (細)...
- 液 : 名詞(液體) liquid; fluid; juice
- 解 : 解動詞(解送) send under guard
- 凍 : Ⅰ動詞1 (液體或含水分的東西遇冷凝固) freeze 2 (受冷或感到冷) feel very cold; freeze; be frostb...
- 精液 : [生理學] semen; sperm; seminal fluid精液保存 semen storage; 精液採集 semen collection; 精液檢查 s...
- 解凍 : 1 (冰凍的江河、土地等融化) thaw; thawing; unfreeze 2 (解除對資金等的凍結) unfreeze (funds as...
-
Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年來,國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究,目前這項技術已達到實用水平,並日益發揮作用;二是對魚類培養的組織細胞冷凍保存,通過魚類細胞的培養、超低溫凍存、解凍后再培養過程,結合細胞工程技術(如核移植、電融合等)進行體細胞育種;大量的研究結果表明魚類體細胞核具有發育的全能性,隨著細胞培養技術、細胞工程技術日益發展成熟,完全具備實現魚類物種種質長期保存的理論基礎和技術條件。Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出,轉移到ep - pendoff管中, 5000甲m離心4分鐘,棄去上清,加入ro川粉碎緩沖液,充分振蕩混勻,在液氮中經過2一3次的凍融循環,迫使卵細胞裂解,加人等量的樣品緩沖液沸水中煮5分鐘,用於westem印跡分析。
分享友人