純化培養 的英文怎麼說

中文拼音 [chúnhuàpéiyǎng]
純化培養 英文
pure culture
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 純化 : purification; purifying; depuration; edulcoration; purify
  1. The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria

    二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響，建立了從土壤中提取總dna的方法，並通過改進使適合於對革蘭氏陽性菌的提取。
  2. Wild filamentous fungi obtained from natural fermented millet catsup were identified as aspergillus oryzae by morphology after purifying iteratively

    摘要反復純化培養自然發酵的粟米醬中分離的野生絲狀真菌，經形態學鑒定，確認其為米曲?菌群的米曲?菌。
  3. Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna

    中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。
  4. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附（ eba ）技術是一種新型的生化分離技術，它集成了固液分離、濃縮和初期純化於一步單元操作之中，可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白，而不必事先除去懸浮的固體顆粒。
  5. The isolation and cufure of the ductal cells of smg in vitro

    實驗結果一、領下腺細胞的分離純化與體外培養、傳代。
  6. Spawn is necessary for both cultivation of edible fungi and tameness of wild mushroom. the reliable method of spawn identification is to produce carpophores by the culture, which is not only time - consuming for domestic fungi but also unuseful for untamed wild mushroom

    無論是食用菌的人工栽培，還是珍貴野生蘑菇的馴化研究，都需要制備菌種並對菌種的真偽進行鑒定，其中最可靠的鑒定方法是誘導純培養菌種產生賴以識別的子實體。
  7. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  8. The molecular library technology has special values compared with conventional techniques for serodiagnostic screening of antigens, especially when the pathogens are difficult to culture artificially or the specific antigen purification is hard

    特別是當病原體不易人工培養或難于制備純化抗原時，這種技術較傳統診斷抗原篩選方法具有明顯的優勢。
  9. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  10. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  11. Exosomes derived from human intestinal epithelial cell ( iec ) could be released from apical and basolateral sides and expressed mhc class i molecules, cd26, cd63 in basal conditions besides mhc class ii in inflammatory conditions. the data showed exosomes might be a mechanism of oral tolerance. exosome - like vesicles isolated from rat iec line, which were pulsed by antigens, were shown to induce antigen - spec

    模型的建立和exosomes的分離與純化我們利用一種c57bl / 6小鼠的3lllewis肺癌細胞系作模型，將3ll細胞皮下注射到c57bl / 6小鼠，待3周時無菌取出腫瘤組織，剪碎後用dmem加10 %的血清在37 ， 5 %的czo中培養， 30小時后收集培養上清。
  12. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  13. A strain of bloom cyanobacterium ( blue - green alga ), microcystis aeruginosa, which dominated in an eutrophic pond, was isolated and purified for physiological study in laboratory conditions. various environment factors, like light, temperature, nutrient, cu2 +, ect were tested for growth and lexicological effects of m. aeruginosa

    在純化培養的基礎上，我們開展了光照、溫度、營養鹽及cu ~ （ 2 + ）對該銅綠微囊藻生長及毒理學影響的研究。光照、溫度及營養鹽對銅綠微囊藻生長影響1
  14. Although the issue of sincerity, nation moral integrity, nation culture self - esteem are no t only chinese teaching subject, simply depend on chinese teaching can not thoroughly solve this problem, but since the chinese language is the carrier of nation culture and nation spirit, it has a superiority that other method can not replace in edifying personality, perfusion spirit, cultivate national self - confidence

    盡管諸如「誠信」 、民族骨氣、民族文化自尊等問題，並不單單是語文教育的問題，單純依靠語文教育也不可能得到徹底解決，但由於漢語言是民族文化和精神的載體，它在培養人格、灌注精神、增強民族自信心方面仍然有一種其他方式所不能代替的優勢。
  15. Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis

    同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。
  16. The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum

    應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。
  17. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  18. Crumbs of nails were isolated from onychomycosis suffers, koh was chosen to be solvent, nail fungi were examined by microscopy, cultured in sabourud, purified by method of concentration grads and method of exsecting cusp of mycelia. finally, sixteen purified strains were ready

    從甲病患者病甲中分離出甲碎屑，選用koh為浮載劑，直接鏡檢，採用沙堡氏培養基對它們進行分離培養，濃度梯度法和切取菌絲尖端生長法對菌種進行純化，得到純化后的菌株16株。
  19. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  20. These are main problems of eutrophication. at first we select algae which grow under different level of nutrition ( or different development phase in the process of eutrophication ), domesticate, selecte seed, and culture algae at the lab, and then we obtain dependence of each algae on nutrition and understand information of eco - breadth of nutrition of supplied alga through pure culture under different concentration of nutrition. we find out resource utilization, competition between interspecies and confirm the quantity - effec t relationship between succession of species and nutrition through mixed culture

    本文首先選取在不同營養環境下（或富營養化過程的不同發展階段）生長的藻類物種，在實驗室內進行馴化、選種與純化培養；然後通過不同營養水平的純藻培養實驗，獲取不同藻種生長過程對營養物質的依賴行為，從而可以了解每一供試藻種的營養物生態幅信息；再通過對應培養濃度的混合培養試驗，以期揭示生物種群間的資源利用與競爭行為，並可進一步確定生物種群的演替與環境營養物之間的量效關系。
分享友人
分享到 Line

分享到臉書