純化蛋白組分 的英文怎麼說

中文拼音 [chúnhuàdànbáizǔfēn]
純化蛋白組分 英文
purified protein fraction
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 純化 : purification; purifying; depuration; edulcoration; purify
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. It was firstly reported in china. the third part rthe capsid protein vp | gene was expressed. 810 bp fragment was inserted into vector pgex - 4tl, and then transformed into e. coli bl21

    這是國內首次對aev基因組全序列的分析；第三部分： aev外殼蛋白vp _ 1基因在大腸桿菌中的融合表達、純化及活性研究。
  3. Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %

    本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。
  4. In the part, the author ' s intention is mensurating the molecular weight of the polypeptides by sodium dodecyl sulfate polyacrylamide gel electro - phoresis. the result shows that one of the polypeptides which we have mensurated is about 17, 000da. the conclusion is that the components which we have separated and purified are small molecule polypeptides. 5

    紅褐林蟻多肽蛋白質的分子量測定本部分旨在通過sds -聚丙烯酰胺凝膠電泳測出所得多肽蛋白質的相對分子量，測得其中一組分的相對分子量為17000da左右，結論為所分離、純化的物質是小分子的多肽。
  5. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  6. This thesis is consists of three parts : 1. the purification of ucp - l ( uncoupling protein - 1 ) from bat ( brown adipose tissue ) of the tree shrew. 2

    本論文主要內容有三個部分： 1 、中緬樹?褐色脂肪組織解偶聯蛋白質- 1的純化。
  7. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  8. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  9. By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles

    對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。
  10. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  11. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  12. Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen, and express the target proteins by iptg induced in escherichia coli

    目的：構建截短的乙型肝炎表面抗原分子的原核和真核表達重組質粒，然後分別在大腸桿菌中誘導表達並純化表達蛋白及在真核細胞中表達目的基因，並檢測其抗原特性。
  13. Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root

    首先，構建了ecbp21表達載體，誘導了重組蛋白的表達，並通過膠回收法獲得了大量純化重組ecbp21蛋白，制備了高效價、高特異性抗體；隨后，利用ecbp21抗體，結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究，結果顯示：在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域，而在細胞內未發現或僅有少量金顆粒分佈，表明ecbp21蛋白主要定位於細胞壁區域，這為細胞外cambp （ ecbp21 ）的胞外存在提供了直接證據：進一步，利用ecbp21抗體，通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況，結果表明ecbp21在白芷各組織中均有分佈，但在葉、花、花序軸中分佈較多，而在葉柄、根中分佈較少。
  14. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  15. Secondly, put acetone in the abstracting liquor, put it aside for one hour and slowly centrifuge. thirdly the deposit is fully dissolved and heated for 15 minutes in 50 c water, and get crude enzyme solution through centrifuge

    分離純化將蘆薈的葉子洗凈、擦乾，加入tris - hcl緩沖液進行組織搗碎提取sod ，高速離心；將提取液用丙酮使sod沉澱、靜止，低速離心；將沉澱用蒸餾水充分溶解， 50水浴中加熱15min ，離心除去部分雜蛋白，獲得粗酶液。
  16. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  17. The recombinant fasl - ecd was purified and its biological activity was analyzed on several cell lines and the most sensitive cell lines are selected. ( 2 ) using a computer program, a single short peptide is derived from antisense homology box of fas ligand and is chemically synthesized. ( 3 ) examine the apoptosis - inducing effect of the recombinant fasl - ecd and the ten - peptide on the most sensitive cell lines, and the relationship between them was analysed

    純化重組蛋白fasl - ecd ，並分析其生物學活性，篩選出對之最敏感的腫瘤細胞株； （ 2 ）根據生物信息學軟體分析結果，選取fasl胞外區256 - 265的十肽（ n ） - hlyvnvsels - （ c ）作為目標分子，委託生物公司合成該十肽； （ 3 ）分析fasl - ecd和十肽對最敏感腫瘤細胞的毒性作用，分析重組fasl一ecd及十膚作用的相關性。
  18. 1. isolation, purification and characterization of lipovitellin from the ovary of female eriocheir sinensis broodstock lipovitellin was extracted and purified using gel chromatography and electrophoresis procedures from ovaries of chinese mitten - handed crab. one kind of lipovitellin was obtained

    一、中華絨鰲蟹卵黃磷蛋白的分離、純化與鑒定目前在判定蝦蟹卵巢成熟和卵質時，大多採用性腺指數、蛋白質和脂類的組成與含量等生化指標，尚不夠全面。
  19. Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption. immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans, then eluted and further purified by columns of thiophilic gel. based on above experiments of antibody extraction, bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus

    首先，利用變異鏈球菌（ s . mutans ）與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體（ igy ） ，並將菌體上洗脫下來的igy用嗜硫色譜分離純化，得到高純度的細菌特異性抗體。
  20. Sectionii : to research and establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies. to raise separate efficiency and biological active efficiency is especially important in the purification model of recombinant proteins. in accordance with these questions, we raise inclusion bodies purity before the purification as far as possible, and select the suitable chromatogram technology

    研究並建立一種包涵體中高效分離純化目的蛋白的優化模式重組蛋白分離純化工藝中分離效率的提高、生物活性效率的提高及工藝的穩定性是尤其重要的，針對這些問題，我們在層析純化前盡山西醫科大學生物化學與分子生物學2003屆碩士學位論文可能地提高包涵體純度，並選擇合適層析方法的同時合理的組合色譜分離單元。
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