純合基因 的英文怎麼說

中文拼音 [chúngějīyīn]
純合基因 英文
homozygous gene
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  3. It was firstly reported in china. the third part rthe capsid protein vp | gene was expressed. 810 bp fragment was inserted into vector pgex - 4tl, and then transformed into e. coli bl21

    這是國內首次對aev基因組全序列的分析；第三部分： aev外殼蛋白vp _ 1基因在大腸桿菌中的融合表達、純化及活性研究。
  4. Most plants carry a large "genetic load" for deleterious recessive genes of which a few are homozygous and depress growth.

    多數植物帶有大量有害的隱性基因的「遺傳負荷」，其中少數是純合的，因而降低了生長。
  5. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  6. Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

    進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。
  7. Abstract : the main characters and seed set of bc1 ， bc2 ， bc1f2 from ms line back - crossing with the pollen of hybrid rice were studied. the appearance of a large number of self fertile plants in ms line was analyzed. the effect of introgression of restoring genes on ms line purity and hybrid yield in indica and japonica rice was discussed. it was pointed out that “ iso - cytoplasm restorer line ” was seriously reducing the purity of ms line and that hybrid rice was unsuitable for isolation zone in the seed production fields

    文摘:研究了雜交稻花粉對母本不育系回交後代群體bc1 、 bc2 、 bc1f2的主要農藝性狀和育性表現，分析了不育系中出現大量自交結實株的原因，討論了恢復基因滲入對秈、粳不育系純度和雜交稻產量的影響，指出「同質恢」對降低不育系純度的嚴重性和雜交稻作制種田隔離區的不合理性。
  8. Among the three methods used in the experiment of dna extraction, only ctab, adding pvp in the dna isolation step, had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process. pcr amplifications were performed in a final volume of 25 mm3 containing 0. 5 units taq polymerase, 2

    通過在抽提緩沖液加入2的pvp 、並用異丙醇和乙醇沉澱基因組dna等改良措施， ctab法能避開大量纖維、多糖的影響，有效地從不同屬種的棕櫚科植物的幼嫩葉片中提取並純化了適合rapd的基因組dna 。
  9. Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions

    本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。
  10. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  11. Of the 21 patients conservatively treated between 1975 and 1997, 12 had diffuse disease ( homozygous mutant alleles ) and 9 had presumed focal disease ( paternal mutant allele only )

    1975年至1997年期間接受保守治療的21例患者中， 12例為彌漫性病變（等位基因突變純合子） ， 9例為局灶性病變（僅父系等位基因突變） 。
  12. Inbreeding increases homozygosity so that deleterious recessive genes are expressed more often in the phenotype, and decreases heterozygosity and hence the potential genetic varability of the population

    近親交配使純合體幾率增加，因此，一些有害的隱性基因可以被表達。
  13. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  14. The probability of homozygosity by descent ( having common ancestors )

    就是說某一個純合個體兩個等位基因來源於同一祖先的概率。
  15. In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method

    為了選育新類型玉米雄性核不育系，提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略，同時應用分期播種的方法為作物提供不同的生長和發育的環境條件，以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明，春播環境下可鑒定和選擇玉米的不育性，夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性，結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性，為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇，能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法，使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系
  16. There are a variety of mechanisms believed to be involved, including genomic modularity - - the ability for animals to reorganize their genome in response to stress or other outside influence, heterozygous fractionation ( heterozygous genes in parents can lead to speciation by having multiple homozygous genes in children ), and standard evolution

    認為涉及很復雜的變化機制-動物重組基因組響應壓力或其它外在影響的能力（雙親基因雜交會導致孩子們擁有許多純合子基因的物種形成） ，而標準地進化。
  17. Their frequencies of gus expression in pollens were 97. 755 % 0. 800 % and 96. 556 % 0. 600 % respectively. to verify these results, the self - pollinated progenies of these two putative homozygotes were examined in t2 by histochenmical gus staining of vegetative tissues and pcr analysis using specific primers

    （ 2 ）通過對轉基因t1代的花粉gus基因表達檢測，快速鑒定和篩選得到了番茄純合植株，加速了純合體篩選的進程，證明此方法可靠，而且不會造成大量寶貴材料的浪費。
  18. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  19. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  20. Most plants carry a large " genetic load " for deleterious recessive genes of which a few are homozygous and depress growth

    多數植物帶有大量有害的隱性基因的「遺傳負荷」 ，其中少數是純合的，因而降低了生長。
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