純合性別 的英文怎麼說
中文拼音 [chúngěxìngbié]
純合性別
英文
homozygous sex-
The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently
利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %
本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1
因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。The gold particles and virus could be seen binded together in the electronic microscope, which indicated the activity of purified igg was high. clinical application of hyperimmunalserun was used to treat dogs with clinicalsigns compatible with canine distemper and parvovitus enteritis
將純化的igg與提純的cdv 、 cav病毒反應後分別與膠體金標記的spa結合，在電鏡下可清楚地觀察到病毒與膠體金顆粒的結合，說明提取的igg的效價、活性較高。Molecular imprinting technique ( mit ) is becoming increasingly recognized as a powerful technique of preparing synthetic polymers that contain tailor - made recognition sides for certain molecules. it has an extensive application in purification, separation, immunoassay, enrichment, biomimics and other relevant fields for its predetermination, specificity and practicability of molecule recognition
分子印跡技術是一種制備對特定分子具有專一識別性能的聚合物（ mip ）的技術， mip對模板分子的識別具有預定性、專一性和實用性等優點而在分離提純、免疫測定、生物模擬以及痕量分析等領域顯示出廣闊的應用前景。Compared with pure pmma, the thermal and mechanical properties of the nanocomposites are obviously improved at a very small amount of mmt ( ommt ) loading. the tgs of the composites via different methods are improved by 10 ~ 26 ?, the decomposition temperatures at weight loss of 5wt % are increased by 42 ~ 99 ?. in the bulk system, tensile strength, tensile modulus and impact strength are improved by 32. 2 %, 60. 3 % and 68. 6 %, respectively, without the loss of light transmittance
相對于純pmma體系，懸浮法、乳液法及本體法復合材料的t _ g值提高了10 26 ；后兩種復合材料熱失重5wt的溫度提高了42 99 ；而本體法復合材料的拉伸強度、拉伸模量及沖擊強度值最大分別提高了32 . 2 、 60 . 3和68 . 6 ，而透光性能基本未受影響。Biology, etc. owing to many merits has not yet been used to measure parameters of gratings. the paper researches on the subject in view of current lack of it. the main tasks of the paper include : analyzing ellipsometric characteristics of gratings in detail with vector diffraction theory and ellipsometrics ; devising a reflective quarter wave plate at normal incidence according to some ellipsometric characteristics ; making use of normal simplex algorithm during ellipsometric inversion of gratings parameters, inversing ellipsometric parameters with gaussian noise of different standard deviations to simulate actually measured values with examples of isotropic metallic and anisotropic step gratings and testing that ellipsometry about gratings parameters is feasible with the range of certain precision ; discussing choice of incidence angle at length
本論文的主要工作包括：結合光柵的矢量衍射理論和薄膜的橢偏理論，詳細分析了光柵的橢偏特性；並且根據一些橢偏特性設計出一款正入射反射型單波長1 4波片；在光柵參數的橢偏反演中，引入正單純形法作為反演演算法，分別以各向同性的正弦形金屬光柵和各向異性的階梯型光柵為例，在標準橢偏值的基礎上加入不同偏差的高斯噪聲來模擬實際的橢偏測量值進行反演，在一定精度范圍內得出滿意的光柵參數，說明光柵參數的橢偏測量是可行的；還就入射角的選取問題進行了一定的探討。In the base of analysis the water conservancy project ’ s management system history and actuality of our country, this paper point out some problems in the system, such as the property is not clear 、 personal exceed 、 the outlay is not enough 、 the system of insurance is not perfect ; bring forward something that can use reference in our country through introduce the water system of america 、 japan 、 holland ; then put forward the combine of construction and operation of water conservancy project, mainly study in feasibility research stage 、 design stage and building stage ; then, analysis the framework of pure - commonweal 、 quasi - commonweal and management water conservancy and point out the management system of water conservancy projects that suit in the conditions of our country. mainly in develop mixed farming 、 deepen in the system of personal 、 make correlation policy accelerate the reform of the water conservancy project system. at last, the author give some advice
本文在分析我國水利工程管理體制歷史和現狀的基礎上，指出我國水利工程管體制存在的諸如單位性質劃分不準、人員超編、工程管理經費不足、社保體制不健全等方面問題;通過分析日本、美國、荷蘭等國的水利工程管理體制，提出可供我國水利工程管理體制模式借鑒之處;進而提出新建水利工程建設管理和運行管理有機結合的方法，主要從可研階段、設計階段、施工階段進行結合;然後按照水利可持續發展戰略思想，探討適合我國國情的三類水利工程管理體制模式，分別分析純公益性、準公益性、經營性三類水利工程管理體制模式，研究出適合我國國情的水利工程管理體制模式;從開展多種經營、深化人事制度改革、制定相關政策等方面促進水管單位管理體制的改革;在文章的最後，提出筆者的幾點建議和展望。The cost of mixed goods, a special type of public goods, can be compensated partially through taxation, which is the usual form of cost compensation for all public goods ; fee collection, the price of mixed goods, also play a vital part in cost compensation ; in addition, there are other forms of cost compensation such as government bond, lottery, mutual fund and price
不同混合產品的外部性是有所區別的，依據不同混合產品所具有的不同的外部性，可以對其進行排列。將混合產品提供主體和混合產品外部性分佈結合起來進行分析，可以對混合產品進行如下分類:政府主導型混合產品:這類混合產品的提供主體主要是公共部門，其外部j睦大，在性質上接近純公共產品。( 4 ) in the research of the correlation between farmer ' s human capital and igfh, the influence of farmer ' s educational degree and technique training to igfh was positively analyzed, it shows that : ( das while as the educational degree of familial main labor is increasing, income of farmer household is growing ; in the meanwhile the correlation between educational degree of familial main labor and farmer ' s income from high technique industry is obviously demonstrated ; ( 2 ) farmer ' s per labor income is increasing as general educational degree of familial labors is increasing, also farmer ' s income is quicken - up as their years of schooling increasing, and farmer ' s per labor income is not strongly correlated with some lines of business ; ( d as for familial labor trained for an special profession, the farmer who received training has more income, also illiterate households had more income after being trained compared with high - schooling households
（ 4 ）在農戶人力資本與其經濟收入增長關系的研究中，實證分析了勞動者文化程度和職業技術培訓對農戶經濟收入增長的作用。結果表明：隨家庭主要勞動者浙江大學博士學位論文農戶投資對其經濟收入增長的作用研究一一以山西省為例文化程度的提高，家庭人均純收入呈增長趨勢，同時主要勞動者文化程度與勞務收入以及在技術含量較高產業的收入之間表現出較強的相關性;隨家庭勞動者綜合文化程度提高，勞均純收入呈增長趨勢，且表現出較為明顯的遞增報酬關系，但與家庭經營內部一些業別的勞均收入之間呈現弱相關性;從農戶家庭勞動者接受職業技術培訓情況看，總體上接受培訓的農戶收入高於沒有接受培訓的農戶收入，且文盲戶接受培訓后收入增長較高中以上戶顯著。Through acquiring the latest international research achievements and our country " s realistic economic situation, our country " s accounting model of irfs could be : to be recognized within the balance sheet ; fair value as the measurement attribute ; to be dealt according to the purpose for which the irfs have been held ; to be reported mainly in the statement and then in the footnotes
通過吸收國際上對投機交易（包括純粹投機、套利和套購三個交易方式）和套期保值交易的最新研究成果，並結合我國特定的經濟環境，提出我國利率期貨投資企業會計的初步設想：在資產負債表中列示、以公允價值作為計量屬性、按交易目的在會計上分別確認和以表內列報為主、報表附註為輔的報告方式。The thesis gives a macro explanation and grasp which is based on theoretical foundation, social surroundings and developing trend, explains the possibility and necessity of its appearance and discusses the developed process of its object which covers not only real property but also all asset. the author analyzes beneficial weight, systematic ideal and legal values, then clarifies that the legal system needs profound juristic basis. by using the method of comparative analysis, the author expounds the requirement of applicable conditions in other countries and analyzes its deficiencies in the contract law of people ' s republic china
本文首先從該制度產生的理論依據、社會背景以及發展趨勢上，對其進行一個宏觀了解和把握，說明其產生的可能性和必要性以及適用對象由不動產向一切財產轉變的發展過程；其次通過利益衡量、制度理念、法律價值三個方面的分析，闡明該制度的存在有其深厚的法理學基礎；又運用比較分析的方法，闡述各國在其適用條件上的要求，分析我國合同法有關此方面存在的缺陷；再就該制度適用后所產生的法律效果，分別分析三方（所有人、出租人、承租人）之中每兩方之間的法律關系與單純的買賣關系、租賃關系在權利義務上有何不同；然後將其適用范圍擴大，類推適用於融資租賃和他物權；最後提出完善我國買賣不破租賃制度的立法上的建議。( 5 ) sic content in coating was 20. 11 ( vol % ). coating was fine and close. the hardness was 2. 68 times pure ni coating, and wear - resistance was 4. 1 times pure ni coating
（ 5 ）對所得鍍層的性能進行了檢測，鍍層中sic粒子含量為20 . 11 （ vol % ）且均勻緻密;鍍層的硬度和耐磨性分別提高到純鎳鍍層的2 . 68倍和4 . 1倍;結合力實驗也表明鍍層與基體具有良好的結合力。The sample composition was analyzed by means of tg and x - ray diffraction. the following conclusions can be drawn from the experiments : given conditions, the sample powder that has the same xrd data as the standard fetioa can be gotten when use any method mentioned above ; the key in synthesis is to avoid the oxidation of fe2 +, so the whole process should performed under vacuum or neutral atmosphere, samples used by this study were prepared under nitrogen atmosphere ; the heat - treated time and temperature have some influence on the sample powder, results show that the proper time and temperature is 120 minutes and 1100 respectively
研究結果表明：在合適的反應條件下，用上述三種合成方法都能得到xrd數據與標準fetio _ 3物相基本吻合的粉體；合成反應需要克服的主要問題是防止體系中fe ~ （ 2 + ）的氧化，因此，整個反應必須在真空或者非氧化性氣體保護下進行，本研究需要的樣品是在高純從保護下合成的；煅燒的時間和溫度對最終的產物也有一定的影響，研究發現：合理的煅燒時間和溫度分別為120分鐘和1100 。Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result
西安理工大學碩士學位論文在水平地震作用下，並考慮風荷載組合，分別對「矩形柱純框架結構」 、 「異型柱純框架結構」 、 「異型柱框一析架結構」及「異型柱框-剪結構」等四種結構體系，通過改變結構參數，運用空間有限元方法，進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ，以及「構件內力」 。結果表明:在等面積原則下，異型柱代換矩形柱后，結構剛度增大，側向位移明顯減小，地震反應力略有增加，框架柱軸壓比最大增幅小於8 % ，結構整體抗震性能有所提高;當異型柱肢長肢厚比小於等於3 . 6時，結構有利於抗震;水平地震作用力與整體坐標夾角為0度時，結構地震作用效應較大，屬結構設計的控制因素之一;異型柱框一析架結構採用「人」字斜撐，側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型柱框一剪結構側向剛度大、位移小，構件內力增大適中，是一種抗震性能優越的結構體系:在抗震設防烈度較高地區（ 8度）和抗震不利的建築場地（ m類場地） ，採用異型柱結構體系時，應盡可能減輕結構自重，降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算，兩種方法所得結果基本一致。First of all we discuss the model of information purifying and bring forward the methods of setting up the according fuzzy set and subject function. secondly after analyzing the traditional technology and the strongpoint and the shortcoing of information purifying we improve it combining with the technique of fuzzy mode identifying, data warehouse, cache etc. and we can perpetrate an on - line and synchronous purifying through analyzing the text and picture showing in the pages of network. finally, we choose sql server 2000 to design the url database and delphi, wingate as the tool for system development to develop an efficient system of information purifying which can keep the network consumer especially young student apart from the intrusion of unfriendly information and make the environment of network pure and fine
本文首先探討了該系統中的信息「凈化」模型，提出了模型中的模糊集及隸屬函數的構造方法；然後分析了傳統的信息「凈化」技術及其優缺點，結合模糊模式識別、數據倉庫、高速緩存等技術對傳統的信息「凈化」技術進行了改進，改進后的信息「凈化」技術可通過分析正在顯示中的網頁文字、圖片內容，做即時、同步性的網頁內容篩選；最後，利用sqlserver2000設計了url數據庫，選擇delphi 、 wingate作為系統開發工摘要具，設計開發了一種高效的網路「凈化」器，使網路用戶尤其是青少年學生遠離非友善信息的侵擾，讓網路環境更加純凈、美好。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。Based on the above - mentioned matter, the application of toc to the product mix problem was studied home in this article. the product mix decision problem was divided into simplex resource constrains and manifold resources constrains by enterprise ’ constrain resources, and their solution steps according to toc ’ s five key steps ameliorated were presented. some material examples were solved by toc ’ s five steps technique, linear programming ’ s simplex method and familiar accounting technique ( variational cost technique and full cost technique )
文章依據企業約束資源的多寡，將產品組合決策問題分單一資源約束與多種資源約束兩種情況進行了討論，通過對約束理論五步驟法的適當調整，給出了它們各自的約束理論解法的解題步驟，結合具體的算例，分別利用約束理論五步驟法、線性規劃的單純形解法和常見的會計學的變動成本法和完全成本法進行了求解，並對約束理論解法、線性規劃解法和會計學解法進行了適當的比較分析，證明了產品組合決策問題約束理論解法的有效性和先進性。分享友人