純種敏育 的英文怎麼說
中文拼音 [chúnzhǒngmǐnyù]
純種敏育
英文
pure line breeding-
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method
為了選育新類型玉米雄性核不育系，提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略，同時應用分期播種的方法為作物提供不同的生長和發育的環境條件，以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明，春播環境下可鑒定和選擇玉米的不育性，夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性，結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性，為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇，能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法，使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系
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