純胰液 的英文怎麼說
中文拼音 [chúnyíyè]
純胰液
英文
ppj-
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。The hyperglycemic activity of pancreatic extracts was encountered some 80 yr ago during efforts to optimize methods for the purification of insulin
約80年前，在嘗試優化胰島素提純技術的過程中人們發現胰腺提取液能引起高糖狀態。Monitoring insulin in procedure of production and purification, determining insulin content from bio - tissue fluid and single islets of langerhans, and studying insulin molecular structure are all dependent on chromatographic analysis
胰島素制劑的純度檢驗，胰島素生產及純化過程的監控，生物體液及胰島細胞分泌胰島素的狀況，重組胰島素結構的剛定及活性研究等都離不開胰島素的測定。After washing with reagent ( 50mmol / l ph8. 0 tris - hcl, 100mmol / l nacl, 0. 5mmol / l edta, 2mol / l carbamide, 0. 2 % triton x - 100, 0. 2 % doc ), ultrasound crushing ( 200 w, 150 times, 3 seconds per time, spacing 3 seconds ) and freezing - melting methods, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %. 4. radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0. 5 unit per litre bacterial liquid
3 .表達產物初步純化仁gly 」一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體沉澱溶於含zmol ， / l尿索洗滌液，超聲（ 200w 、巧o次、 3秒/次、間隔3秒） ， 4 、 i000orpm離心15min后沉澱用洗滌液充分重懸，一2 （ ）凍存過夜，次日融化后4 、 10000rpm離心15min ，取上清，即得到初步純化的融合蛋白，可去除土要的雜蛋白，融合蛋白溶解于上清液中，純度達到80 %以_ 1 : 。分享友人