細粒產物 的英文怎麼說

中文拼音 [xìlìchǎnwù]
細粒產物 英文
fine product
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 產 : Ⅰ動詞1 （人或動物的幼體從母體中分離出來） give birth to; be delivered of; breed 2 （創造財富; 生...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  1. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  2. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  3. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  4. It was show in this thesis that the po activity existed histochemically in haemo - cyte and its peripheral zone. most po were electron - dense and homogenous. prophenoloxidase is found in both plasma and haemocytes. propenoloxidase is most abundant in large granule haemocytes, a small abount is present in some small granule haemocytes and hyaline cells, or absent

    Po大部分均質且電子密度很高。在細胞外有異物處的po密度最大。其中大顆粒細胞周圍的po陽性產物最多，小顆粒細胞和透明細胞則很少或沒有。
  5. V. experimentation and pilot test of the whole flow of preparation of ultra - fine active zinc oxide by comprehensive utilization of zinc dross have been completed

    採用日本島津產epma - 1600型電子探針對產物超細活性氧化鋅的微觀結構、粒度和形態進行表徵。
  6. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  7. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  8. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
  9. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  10. The study solves engineering problem as follows : ( 1 ) the systematic study on the historical course of xigeda strata deposit and geologic environment includes strata, lithologic characters and constitution et in the liangshan and panzhihua region. the results show that the xichang - panzhihua express way is controlled by the anninghe fault and the xigeda fault, the earthquake frequently happen in the region of pass, the different sedimentation number is more great, they effect the road building, safety in operation and structure belong road ( 2 ) because in some place the xigeda strata is foundation and roadbed, the study on the basic properties of the xigeda strata include density, moisture content plastic and liquid limit graduation. the results show that the xigeda strata is deposition in lake, the main component is mudstone and sandstone, the fine particle is main, it has some viscosity, the moisture content of mudstone is different the it of sandstone, the other property are likeness. the xigeda strata has no dilatation on total, some claystone have low and medium dilatation ; ( 3 ) the study on the basic properties of xigeda filler include the composition of matter the biggest standard dry density, the optimum moisture content the results show that xigeda filler can use as road material, it is well grade filler and admixture filler on essence, its compaction index should change in different place because the property is controlled by mudstone and sandstone that change is great in different place ; ( 4 ) the study on the xigeda filler craft used as express way roadbed, includes suitable thickness, compaction numbers and methods and equipment choice et

    公路沿線廣泛分佈有昔格達地層，昔格達地層能否用作高速公路路基填料，國內沒有先例，本論文就是結合導師的科研項目，在非典期間，現場長達5個月的工作，完成了從試驗、現場施工工藝到路堤分層沉降監測等工作，是面對生產實際，認識昔格達地層的性質和其作為地基、路基和填料應用中面臨的問題進行的研究。主要的研究內容有：對攀西地區地層巖性、構造等地質環境和昔格達地層的沉積歷史過程作了系統的分析，研究表明西攀高速公路主要受安寧河斷裂和昔格達斷裂影響，通過地區地震活動頻繁，差異沉降較大，對公路建設、安全運行和沿線構築物設計有較大影響；由於昔格達地層在有些路段作為地基、有些作為路基，對其基本性質進行了研究：包括天然密度、含水量、塑液限、顆粒級配等指標的試驗研究。研究成果表明：昔格達地層為湖相沉積，主要為砂巖和泥巖，它以細粒組為主，同時粘粒的存在，使其具有一定粘性。
  11. Examined by tem, the production was slice shape in which there are many fine grains and the size of slices is 200nm ~ 2 u m and the most part of it is 500nm when the quality match ratio of aluminum nitrate and urea was 2. 5 : 1 and raw materials was ignited at 500 c. the size of slices is 200nm ~ 400nm when the quality match ratio of aluminum nitrate and urea was 2. 5 : 1 and raw materials was ignited at 300c

    經tem分析發現產物形貌呈片狀，當硝酸鋁和尿素在質量配比為2 . 5 ： 1 ，在500點燃時片狀產物尺寸約為200nm 2 m ，其中大部分粒徑約為500nm ，當硝酸鋁和尿素在質量配比為2 . 5 ： 1 ，在300點燃時產物尺寸約為200nm 400nm ，片狀產物內部由細小顆粒組成。
  12. The fluorescence intensity in high - passage cells was increased obviously ; whereas the mitochondrial membrane potential was decreased obviously

    結論內皮細胞在復制性衰老過程中，伴隨著細胞形態退化、細胞周期異常，細胞內自發熒光產物增加，線粒體膜電位降低。
  13. [ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t

    L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。
  14. The experimental conditions including the types and concentration of protective agents, feeding order and the ph of the solution that influence the average particle size was studied in detail

    詳細考察了ni ~ （ 2 + ）濃度、分散劑濃度、 ph值及加料順序對產物組成和粒徑大小的影響。
  15. The experimental conditions including the types and concentration of protective agents, feeding order and the ph of the solution that influence the average particle size have been studied in detail

    詳細考察了分散劑濃度、 ph值及加料順序對產物組成和粒徑大小的影響。結果表明，控制n廣、 c 。
  16. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
  17. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  18. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
  19. Polymer - network gel process was used to synthesize nanometer oxide such as zro2 ( 3. 5mol % cao ), co3o4 and nio. dta / tg, xrd and tem were used to characterize the gel and products, and determine the lowest temperature and time for calcining the gel. the effect of the concentration of starting solution, temperature and time for calcining the gel on the size of the products were also discussed

    本文採用高分子網路凝膠法進行納米zro _ 2 （ 3 . 5mol cao ） 、 co _ 3o _ 4 、 nio等納米氧化物超細粉的軟化學合成，利用dta tg 、 xrd和tem等分析手段對凝膠和產物超細粉進行表徵，確定凝膠的最低煅燒溫度和煅燒時間，並探討起始無機鹽溶液濃度、凝膠的煅燒溫度和煅燒時間對產物超細粉粒徑的影響。
  20. In 200mol / l cisplatin, no telomerase inhibition was found when cells were collected only after 4 hours of drugs treatment, but the telomerase activity of cells was completely inhibited by cisplatin when removed the drug and recultured for 20 hours. however, vitamin c had no effect. in 8mol / l cisplatin, it was similar with 200mol / l cisplatin to uppress the telomerase acting of caov3

    Ddp對端粒酶活性的影向：當200mol lddp作用caov3細胞4小時，即刻收集細胞，藥物未對細胞端粒酶活性產生抑制；在細胞經過4小時ddp處理，去除藥物再培養20小時，端粒酶活性完全抑制； 8rmol lddp作用細胞24 j 』時，端粒酶活性部分抑制；當sumol lddp作用細胞120 j 』時，對端粒酶活性顯著抑制? 2 ?作用。
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