細胞亞株 的英文怎麼說
中文拼音 [xìbāoyǎzhū]
細胞亞株
英文
cell substrain- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 株 : Ⅰ名詞1. (露在地面上樹木的根和莖) root and stem of a tree above the ground 2. (植株) individual plant; plant Ⅱ量詞(棵)
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302
選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜,並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 : 88 : 22等培養再生植株進行基因表達的微陣列檢測,結果表明: ( 1 )三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays. this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content. the calli of the transformed sublines a78 - 3, a78 - 4, a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以轉基因亞系a78 - 3 、 a78 - 4 、 a78 - 5和未轉基因對照a95 : 88 : 22的細胞愈傷組織為實驗材料,以dkm和lp - m熟化培養基培養五周后,再以1 4sh萌發培養基培養四周,成功地誘導形成了胚胎,並再生成新的小植株,萌發成活率達到80 。Identification of functional bacteria showed predominant ammonifiers were shewanella, variovorax, chryseobacterium, bacillus or aeromonas ; among 4 selected nitrogen fixers, one ( azorhizobium caulinodans ) belonged to. a - proteobacteria, the other three ( serratia marcescens, klebsiella pneumoniae and citrobacter freundii ) were enterobacteriace, which belongs to - proteobacteria ; 2 nitrate reducers were aeromonas sp. and citrobacter sp.,
對各功能菌群中的優勢菌的鑒定表明,優勢的氨化細菌為希瓦氏菌屬,產堿菌屬,黃桿菌屬,芽孢桿菌屬或氣單胞菌屬;分離到的4個優勢固氮細菌菌株中,一株為基瘤固氮根瘤菌,屬于-變形菌亞門,而另外3株都屬于腸桿菌科,歸于-變形菌亞門。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,並促進表達th1 、 th2細胞因子。Professor franky l chan of the department of anatomy explained, previous studies of prostate cancer cells in our laboratory had shown that the err subtype known as err is present in normal human prostate epithelial cells but exhibits reduced expression in many prostate cancer cell lines and clinical neoplastic prostate tissues
中文大學解剖學系教授陳良教授解釋:過去我們在實驗室研究發現這種雌激素受體相關受體亞型( err )在正常人的前列腺上表達,但在一些前列腺癌細胞株和臨床前列腺腫瘤中err表達減少。In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes, some work were done. the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment. the somatic embryos were induced from callus cultured in mise for 35 days. the shoots were induced from cotyledon after cultured in misc 20 days, and from leave which were cultured in misl. the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 %
為篩選水母雪蓮代謝突變體和轉基因研究奠定了一定的基礎,陳亞瓊: m質粒介導水母雪蓮的遺傳轉化及毛狀根中決刪合成的調節p義摘要也為研究類黃酮代謝途徑的關鍵酶基因的轉化、高效表達及作用機理提供了理想的實驗體系。主要的實驗結果如下: 1水母雪蓮高頻再生體系的建立通過體細胞胚胎發生途徑和器官發生途徑,水母雪蓮( squssureamedusamaxim )可以在常溫下獲得再生植株。The optimal expressing time with the calcium phosphate method was 72h after transfection, and the transfection method of calcium phosphate is proved to be more effective than the lipofection method
結果表明,采碩士論文戴君勇: k亞科動物的親緣關系及hsblys的真核表達用cos 7作為真核表達細胞株,其表達目的蛋白的最佳時間是72小時。The phylogenetic tree was constructed based on sequence comparison and analysis. the result showed that c strain derived from rabbit spleen tissue, c strain adapted to sk6 cell, shimen virulent strain all belonged to group 1. 1, and the prevalent virulent strains belonged to subgroup 2. 1 or subgroup 2. 2. this indicated there were at least two subgroups, which were prevailing in china, and the prevalent virulent strains were diverse far from c. strain vaccine virus
通過序列比較和系統發生關系分析發現: c -株兔組織毒、 c ?株細胞毒和中國50 60年代流行的石門強毒株同屬于組群1 ( group1 ) ;近期豬瘟流行毒株均屬于組群2 ( group2 )的兩個不同的亞組群( subgroup2 . 1和2 . 2 ) ,表明我國目前流行毒株至少有2個亞組群。Study on gene expression profile of subclonal cell lines in two different anchorage - independent uterine cervical carcinoma
不同表型的人宮頸癌細胞亞克隆株的基因表達譜The results revealed that antigenic drift had occurred in virus strains of the same h9n2 subtype in the north of henan province during 1998 - 2002. h9n2 subtype aivs were stable from 1998 to 1999, since the mutual protection between 98a5 and 99s approached to 100 %, which was consistent with results in hi and vn tests
1998年和1999年h _ 9n _ 2亞型禽流感病毒較穩定, 98a5和99s毒株間的保護力接近100 ,在hi 、雞胚中和、細胞中和試驗結果的相關性均在0 . 74以上,表明兩毒株間的抗原性相近。Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1
Hsp70是一種分子伴侶,主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復,對線粒體結構和功能發揮重要作用,已有研究證明hsp70與動植物的發育有密切的關系,本研究將hsp70正、反義cdna構建成表達載體,並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草,試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株,以證明hsp70反義cdna能創造雄性不育系。分享友人