細胞感受性 的英文怎麼說

中文拼音 [xìbāogǎnshòuxìng]
細胞感受性 英文
cell competency
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 感 : Ⅰ動詞1 （覺得） feel; sense 2 （懷有謝意） be grateful; be obliged; appreciate 3 （感動） move; t...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Enhancement in activities of calcium activated potassium channels in ca1 pyramidal neurons of rat hippocampus after transient forebrain ischemia

    通道感受細胞內的代謝狀態，進而調節細胞膜的興奮性。
  3. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  4. Influence of hypoxia - inducible factor - 1 alpha on the susceptibility of oral squamous cell carcinomas cell lines against chemotherapeutic drugs and radiation

    對口腔鱗狀上皮癌細胞株接受放射和化學藥物治療感受性的影響
  5. Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control

    目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響，探討內毒素影響體外受精結局的環節及可能的機制，確定三個體外培養系統對內毒素的敏感性，以期為臨床和實驗室質量控制提供參考。
  6. Part i effects of gnt - v on cell proliferation, cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method, it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated, and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased. oppositely, these parameters were reduced in cells transfected with antisense cdna of gnt - v. these results suggested that cell proliferation and dna synthesis were modulated by gnt - v

    第一部分gnt - v對h7721細胞生長、 egf敏感性和egf受體的影響用mtt方法發現轉染正義gnt - vcdna的h7721細胞增殖速度加快，而且無論是~ 3h - tdr的總參入量或每個細胞的參入量均見增加，說明dna合成增強，而轉染反義gnt - vcdna的h7721細胞則完全相反， dna合成和細胞增殖速度均見降低，這提示gnt - v可調節細胞的生長。
  7. Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group

    此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。
  8. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  9. Myocardial bioelectric phenomena and their mechanism, the characters of excitation transmission in heart ; the periodicity changes and their characters of myocardial excitability, autorhythmicity and pacemaker of heart ; pumping function of heart and evaluation ; the concept, mechanism and influencing factors of arterial blood pressure ; carotid sinus and aortic arch baroreceptor reflex

    心肌細胞（工作細胞和自律細胞）的生物電現象及其形成的機制，心內興奮傳播的特點；心肌興奮性的周期性變化及其特點，自律性和心臟的起搏點；心臟的泵血功能及其評價；動脈血壓的概念、形成機制、影響因素；頸動脈竇和主動脈弓壓力感受性反射。
  10. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  11. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  12. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  13. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  14. L8 % cells exhibited sensitive to moving stimulation and 7 % cells were direction seiective cells. about 58 % neurons were orientation selective cells, and most of these cells preferred to the stimulus of horizontal orientation. ( 3 ) a weak local fieid potential that induced by electrical stimulation of the basai amygdaioid comp1ex was observed on the suffoce of visual area l, and its latency was only l - - 2 ms

    12 （ n = 108 ）的神經元感受野為同心圓構型，約1的細胞感受野表現出均勻性特徵， 18的神經元為運動敏感性細胞， 7的細胞對不同方向的運動光刺激產生放電活動明顯不同的反應，多數神經元（ 58 ）表現出朝向選擇性，並且集中表現在對水平方位中文摘要』 2 ?的光刺激敏感。
  15. The interleukin - 6 gene polymorphism in patients with coronary heart disease in chinese population

    白細胞介素4及受體基因多態性與哮喘易感性研究
  16. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
  17. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  18. In this paper a multi - layer neural model is designed based on the multi - scale receptive fields of ganglions in retina. the model can keep watch on the periphery part of a scene while processing the center information of the scene. and why it can balance the hardware complexity, processing precision and computational intensity is analyzed

    本文通過模擬人類視網膜神經節細胞的信息處理機制，獲得多尺度的感受野分佈，設計並實現了在對視野中心區感興趣的信息進行處理的同時，對周邊信息保持一定警覺的層次網路模型，為實現機器視覺系統的實時性和避免巨量計算提供有價值的啟示。
  19. Rossi dj, bryder d, seita j, nussenzweig a, hoeijmakers j, weissman il. deficiencies in dna damage repair limit the function of haematopoietic stem cells with age. nature, 2007 jun 7 ; 447 ( 7145 ) : 725 - 9

    因此他們認為造血幹細胞對于非同源端接通道缺陷的這種敏感性是其維持對抗生理壓力，以及在培養和移植過程中受到的損傷的這種能力的一個關鍵決定因素。
  20. It is known that astrocytes take a key role in the response to hypoosmotic stimulation : astrocytes can perceive the change of osmotic pressure and release taurine which then activates glycine receptors on the neurons, and inhibits the release of vp from the neurons in son

    現已發現星形膠質細胞在對低滲刺激的反應中起關鍵性的作用：星形膠質細胞感受滲透壓的變化，通過大量釋放牛磺酸（ taurine ） ，作用於神經元上的甘氨酸（ glycine ）受體，從而抑制神經元釋放血管加壓素（ vasopressin ， vp ） 。
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