細胞熒光分析 的英文怎麼說
中文拼音 [xìbāoyíngguāngfēnxī]
細胞熒光分析
英文
cfa- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 析 : Ⅰ動詞1. (分開; 散開) divide; separate 2. (分析) analyse; dissect; resolve Ⅱ名詞(姓氏) a surname
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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3. observe the binding of oligochitosan labeled with 2 - amac with macrophages under confocal laser microscope and analysis the fluorescence intensity by flow cytometer using the cell quest software
在激光掃描共聚焦顯微鏡下觀察2 -氨基吖啶酮標記殼寡糖與巨噬細胞的結合,用流式細胞儀分析熒光強度。The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis
本研究採用的主要實驗方法:通過熒光顯微觀察、電鏡超微結構觀察確定cbt (淡水白鯧臀鰭細胞)和cp (草魚胚胎細胞)在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage
在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。Based on the theoretical analysis and experimental researches, it is presented that the wider spectra are resulted from the many fluorophores with large numbers of vibrational energy levels on the ground level in the blood cells, and the reduction of the spectral intensity is due to the reabsorption of the blood cells and the energy transfer of the collisions between the fluorophore and another one or other macromolecule. on the other hand, when the concentration of the blood cells is increased, the reabsorption of the blood cells, the secondary fluorescence due to the reabsorption and the influence of the concentration on the energy levels of fluorophores are all the factors of the red - shifted spectral peaks
在進行理論分析和研究的基礎上,提出了因血細胞中存在多種熒光團,且這些熒光團的電子能級上又存在大量的不同的振動能級,從而導致被激發的熒光團發出較寬的熒光光譜;血細胞濃度的增大,熒光團以及其他大分子之間的距離變小,造成它們之間因碰撞的能量轉移概率加大,因而易產生熒光猝滅,結果導致熒光強度的變小;血細胞溶液中重吸收所導致的熒光猝滅和二次熒光發射,以及血細胞濃度的變化對其中熒光團能級系統的影響都是導致熒光峰值波長「紅移」的原因;進而研究了led光誘導血細胞產生熒光光譜的機理。Chapter two is the research results and discussion, which consist of distributions of cell density, fluorescent characteristic per cell of ultraphytoplankton. synechococcus and picoeukaryotes are abundant in all stations of east china sea and yellow sea, and prochlorococcus ca n ' t be found in near - shore stations
第二章為在東、黃海所做工作的主要成果闡述,主要分析了由流式細胞計獲得的超微型浮游植物細胞密度、單細胞熒光在各站位的分佈特徵,結果如下:聚球藻( synechococcusspIn our research, marked autologous fluorescent blood red cell is immitted into sd - rat body and the whole process is shown and recorded by microscope image system. after these processes, we can replay the recorded tape and sample images with video image card. then, we use sequence image processing to analysis the image of dark ground microscope
利用做過熒光標記的自身紅細胞注入sd大鼠體內,通過顯微圖象系統將整個過程以視頻信號的形式存貯,然後利用基於視頻圖象的採集卡,將流速變化過程回放采樣,得到暗視場下的熒光圖象,利用圖象分析和處理的方法,測定血流速度。Methods : the effects of different neurotrophic factors on the growth and differentiation of neural stem cells were observed by cells counting and immunofluorescence staining. the levels of rara mrna and rxra mrna in differentiated neural stem cells were assayed by rt - pcr. agarose gel electrophoresis and image analysis
方法應用細胞計數和免疫熒光細胞化學法,研究不同神經營養因子對神經幹細胞增殖及分化的影響;應用rt - pcr 、瓊脂糖凝膠電泳和紫外分光圖象分析法檢測神經幹細胞分化過程中rar和rxr mrna表達量的改變。結果1In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用,本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ,並將提純的人精子甘露糖受體( purifiedmannosereceptor , pmr )作用於去透明帶的金黃地鼠卵母細胞,運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素( tritc - lca )免疫熒光標記技術觀察pmr對卵子的影響。And the mechanism of action of medicine to cell can be researched form biomedicine ; ( 3 ) the ca2 + concentration in b16 cell was measured with ratio fluorescence imagemaster. the preparation of cell and the carry method of fluorescence indicator were confirmed ; the acquisition of cell image and the ratio analysis method by software - imagemaster were established ; the acquisition of cell fluorescence image and the ratio analysis method by software - felix were established
( 3 )通過用fura - 2 / am對b16細胞內ca ~ ( 2 + )濃度的測量,確定了b16細胞的制備及熒光染料的載入方法;建立了用隨機軟體imagemaster獲取被測細胞的圖象及進行比率分析的方法,用隨機軟體felix獲取被測細胞的熒光圖譜及進行比率分析的方法。In this dissertation, jurkat cells, hela cells and mouse spleen t lymphocytes were chosen as experimental materials to answer the question. with the aid of various techniques such as elisa, immuno - co - precipitation, indirect immunofluoresent co - localization, double - labeling immunoelectron microscopy and so on, the relationships of baf complex with nf1 / ctf and rna polymerase ii were careful observed and analyzed
本文以jurkat細胞、 hela細胞和小鼠脾t淋巴細胞為研究材料,通過酶聯免疫吸附實驗( elisa ) 、免疫共沉澱、免疫熒光共定位和免疫電鏡雙標記等實驗,觀察和分析了baf復合物與轉錄因子nf1 ctf和rna聚合酶在基因轉錄活動中的相互聯系。In hela cells, by using anti - brgl antibodies, anti - rna polymerase ii antibodies and anti - nfl / ctf antibodies, the core subunit brg1 of baf complex and rna polymerase large subunit were found well immunofluorescently co - localized, while nf1 / ctf and rna polymerase large subunit were poorly co - localized. brg1 and nf1 / ctf were also well co - localized. in order to further reveal the relationships of baf complex with nf1 / ctf and rna polymerase ii large subunit at the ultra - microscopic level, we performed the double - labeling immunoelectron microscopy experiment with hela cells
以hela細胞為材料,分別用抗brg1抗體、抗rna聚合酶抗體和抗nf1 ctf抗體進行免疫熒光共定位分析,發現baf復合物的核心亞基brg1和rna聚合酶的大亞基存在很好的熒光共定位現象, nf1 ctf和rna聚合酶的大亞基之間的共定位現象不明顯, brg1和nf1 ctf也有很好的共定位現象。The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
將所構建的單鏈抗體基因克隆入綠色熒光蛋白融合表達載體pegfp - n3 ,瞬時轉染cos - 7細胞,分析其表達情況。To further understand its mechanism of internalization. methods and results 1. a new peptide sequence was designed based on the analysis of the molecular composed of the membrane penetrating peptides using the peptool life software
最後在計算機photoshop軟體上,測量細胞熒光灰度值,並應用spss軟體進行統計分析,研究其穿膜能力與溫度、濃度、時間及細胞功能狀態之間的關系。Not only can it be excited by visible light but also displays a spectrum - shift upon binding with ca +. moreover, it targets precisely into cytosol only, and thereby exhibits great advantage beyond flou - 3 and ruro - 2. thus it is possible to use stdin - am to measure real - time variation of [ ca2 + ] in cytosol
同時細胞熒光圖像分析顯示, stdhi一am進入細胞后只標記胞漿c擴+而不標記胞核c擴+ ,是目前唯一的可以am酉旨型無損傷導入細胞、雙波長、可見光激發的胞漿特異性c擴+熒光探針。分享友人