細胞獲得的 的英文怎麼說
中文拼音 [xìbāohuòdede]
細胞獲得的
英文
cell-derived- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 獲 : Ⅰ動詞1. (捉住; 擒住) capture; catch 2. (得到;取得) obtain; win; reap 3. (收割) reap; harvest Ⅱ名詞(姓氏) a surname
- 的 : 4次方是 The fourth power of 2 is direction
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
- 獲得 : 1. (取得; 得到) acquire; gain; obtain; win; achieve 2. [生物學] acquisition
-
Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded
經pcr檢測,從864個轉化子中獲得了4個陽性克隆,測序表明,人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上,並獲得了可進行細胞融合的重組yac載體。The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma ; it was 88. 2 % in the growing period of 4 ( superscript th ) generation cells and the stains of alb and ck19 were positive in immunocytochemical
所獲得的原代細胞共傳8代,細胞呈小梭形或三角形,體積較小,胞核較大,而胞漿較少;第4代細胞中進入生長期的細胞約佔88 . 2 % ;免疫細胞化學染色顯示細胞胞質中alb 、 ck19染色陽性。4 ) after the activated nuclear transfer embryos had been cultured in m199 ^ and czbm for 48h, the cleavage rate can reach at 16. 41 % and 17. 41 %, and the morula rate were 7. 46 %, % and 6. 74 %, respectively
08融合率:血請饑餓法和nocodazole法處理的供體細胞構建的重組胚胎,經6 dmap乙醇ccb激活,分別獲得17 33o和17 02o的卵裂率以及6According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l
將菟絲子乾粉用75乙醇浸泡后,減壓蒸干后得到褐色漿狀物,經石油醚、氯仿、乙酸乙酯、正丁醇萃取,經柱層析后,再用葡聚糖凝膠對有效成份進一步純化,獲得了菟絲子中能誘導pc12細胞分化的活性組分。Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術,檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。Whereafter a total of fifteen mouse oocytes were injected with round spermatids and seventeen with elongated spermatids
將獲得的小鼠精子細胞進行卵胞質內注射,共穿刺32個小鼠卵子, 15個行rosi和17個行elsi 。Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter. lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv, raise the level of serum antibody. they make chickens obtaining strong immunocompetente in a short time. agp liters of the group of infecting 0. 4mlcbs + ibdv of germinal and 0. 8mlcbs + ibdv of cellcular or 0. 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely. the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method. the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv. they may also alleviate the immunological injury of activated virus to bursa of fabricius. and promote the repairation of the lesion. it can be found that bs can raise body weight gain and feed coversion ratio
將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞,同時頸部皮下注射ibd胚毒或細胞毒滅活苗,結果表明:雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間,提高抗體水平,使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞,結果表明:不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用;還可以減少弱毒對雞法氏囊組織的損傷,加快其修復。The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo, obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
該毒株是將eiavl株( eiavl )通過驢體傳代,使其毒力明顯增強,然後在驢白細胞培養物上連續傳代致弱獲得的。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in
從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明,該基因雖然有著細微的種族特異性,但高度保守並獲得了它的截斷型重組蛋白,根據蛋白一級結構和同源比較方法,模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬,預測了它們之間相互作用的特徵。The ethical snag is that the best stem cells are harvested from human embryos, killing them
最好的幹細胞是從殺死的晶胚上獲得的,這成為幹細胞研究倫理道德方面的障礙。The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf
誘導后,培養液上清通過western - blotting分析證明,轉染細胞表達並分泌出人乳鐵蛋白,分子量為34kd ; elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞;抗菌實驗表明,所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用,而且比人乳鐵蛋白標準品作用更強。As a result, more than 97 % of the testicular cells remained their viability after enzymatic digestion
結果組合酶消化后獲得的睪丸細胞懸液中, 97以上的細胞仍然存活。Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released. while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types
最常用的是機械方法,即利用細針將活檢獲得的小塊睪丸組織撕開,釋放出精子細胞;也可利用酶將睪丸組織消化成細胞懸液,其中包含所有類型的生精細胞。The somaclonal variation of regeneration plant derived from mature embryo of perennial ryegrass ( lolium perenne l. were analyzed with rapd makers
摘要以多年生黑麥草成熟胚誘導獲得的再生植株為材料,利用rapd標記方法對其體細胞變異進行了研究。From more than 430 attempted fusions, 277 reconstructed embryos were made in this way ; of these, only 29 survived to the stage that they could be returned to foster mothers, and only one survived to term
在所嘗試的430多次的細胞合成中,有277個重組的胚胎是以這種方式獲得的;其中,只有29個存活到能被植入養母子宮的階段,最後能足月分娩的只剩一個了。We get the continuous contour curve after using active contour model ( gvf snake )
對于分割后獲得的細胞核輪廓利用活動輪廓模型進行了優化,從而獲得連續的細胞輪廓曲線。Chapter two is the research results and discussion, which consist of distributions of cell density, fluorescent characteristic per cell of ultraphytoplankton. synechococcus and picoeukaryotes are abundant in all stations of east china sea and yellow sea, and prochlorococcus ca n ' t be found in near - shore stations
第二章為在東、黃海所做工作的主要成果闡述,主要分析了由流式細胞計獲得的超微型浮游植物細胞密度、單細胞熒光在各站位的分佈特徵,結果如下:聚球藻( synechococcusspThe am fungus could obtain adequate carbon resources for itself, thus ensuring normal growth and metabolic activit y owing to the efflux of carbohydrate from the plasma membrane of the host root cortical cells to the interface of the symbiosis. thirdly, the structure and composition of the root cortex cellular plasma membrane kept good integrity and the leakage of carbohydrate maintained at low level when the plant obtained enough p and metabolizes normally. although enough carbohydrate was synthesized by plant, the am fungi could not obtain enough energy, resulting in inhibition of growth of the extraradical hyphae and decreased metabolic activity of the am fungi
由此,我們認為植物磷營養狀況調控菌根真菌生長和代謝活性的機制是:當植物處于嚴重缺磷脅迫時,不能合成足夠的碳水化合物供給自身生長的需要,也不能向真菌提供額外的碳水化合物,因而菌根真菌生長受阻,代謝活性較低;當植物中度缺磷時,根皮層細胞質膜的組成和結構不完整,通透性大,碳水化合物通過質膜向外的滲漏量大,菌根真菌因而獲得充足的碳源,保證真菌正常的生長和旺盛的代謝;當植物獲得足夠的磷而使體內磷營養代謝正常時,其細胞質膜組成和結構完整,碳水化合物的滲漏量保持在較低的水平,盡管此時植物合成了足夠的碳水化合物,但菌根真菌仍不能獲得足夠的能量,導致真菌生長受阻和代謝活性降低。3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin
對于秦川牛皮膚上皮細胞與成纖維細胞的純化: dispase冷消化單細胞培養的效果最佳,可獲得純化的上皮細胞系,而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜於獲得純化的成纖維細胞系。分享友人