細胞轉染劑 的英文怎麼說
中文拼音 [xìbāozhuǎnrǎnjì]
細胞轉染劑
英文
cytofectin- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 轉 : 轉構詞成分。
- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 劑 : Ⅰ名詞1 (藥劑; 制劑) a pharmaceutical or other chemical preparation 2 (某些有化學作用的物品) a...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Dosage compensation in female mammals is achieved by inactivating the transcription of one of the x chromosome
雌性動物的劑量補償效應是通過細胞中一條x染色體的轉錄失活來實現的。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞,進行瞬時表達, 48小時后,用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。The common inducing protocols, including specific inducers, five - step induction, stromal cell coculture and gene transfection, are not very effective, with the shortcomings of complicated manipulation and high costs
目前常用誘導方法包括使用特異性誘導劑、 「五步誘導法」 ,還有尚有爭議的基質細胞共培養法,以及基因轉染與篩選,但有操作復雜、費用高、毒性大等缺點。
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