細胞進化 的英文怎麼說
中文拼音 [xìbāojìnhuà]
細胞進化
英文
cytocatalytic-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏染色,計算t淋巴細胞轉化率,計算免疫器官指數,測定肝臟、脾臟銅含量。Red blood cells were imged by afm and the typical biconcave shapes were observed. the ultrastructure of rbc membrane was also gained. the morphological changes of red blood cell ( rbc ) membrane after the interaction of trichosanthin ( tcs ) were clearly observed by afm
對紅細胞進行成像,觀察到紅細胞雙面凹的典型特徵,小范圍掃描觀察到了紅細胞膜表面的超微結構,在此基礎上研究了紅細胞與天花粉蛋白作用后紅細胞膜表面超微結構的變化,並對二者作用的機理進行了分析討論。Study of " ph memory " and activity water ( aw ) in processing of immobilization indicates that former factor has much effect on transformation and the latter just has little effect. permeabilization treating of baker ' s cell with alcohol and ultrasonic leads to lower transformation rate, which illustrates that permeabilization procedure has negative role on biotransformation with whole cell as biocatalyst in organic solvent. in simple dynamics research amount of biocatalysu concentration of substrate and reaction course are considered and all of them have remarkable effect on transformation
Ph記憶、水活度及通透性處理的實驗結果表明, ph記憶對轉化有明顯的影響,水活度對模型反應的影響不大;作為完整細胞生物催化,細胞的通透性可能是傳質阻力的重要組成部分,利用不同濃度的乙醇和超聲波對細胞進行處理,結果表明,在有機相完整細胞生物催化中,通透性處理對轉化產生的是負面影響。Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively
有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域,目前該領域的研究大多集中在利用分離的酶進行生物催化,利用微生物完整細胞進行的研究比較少。The mensuration of the biologic activity of the formica rufa linnaeus polypeptides. in the part, we used the experiments in vitro studies, the lymphocyte transformation, to mensurate the biologic activities of the polypeptides whose contents are higher than others ". the conclusion is the polypeptides have the immunological activity
紅褐林蟻多肽蛋白質的生物活性測定本部分用體外實驗的方法? ?淋巴細胞轉化實驗對分離、純化所得含量較高的多肽蛋白質的生物活性進行測定,結論為所得到的多肽具有抑制淋巴細胞轉化的免疫活性。The karyotye analysis was made on the 5th passage, the number of the chromosomes ranged from 187 to 200, and no heteroploid cell was found
對第5代的傳代細胞進行了遺傳學分析,傳代4次之後,其染色體形態正常, 4n為187一200之間,未發現明顯的異倍化現象。Intracellular lipids turn to peroxide lipids, causing dryness of skin stimulation of melanocytes and damages epithelial cells and dna, giving rise to blotches
基底細胞膜及dna的損害,使細胞老化不活性化,也會進一步引起皮膚癌。For the purpose of exploiting the affluent resource of traditional chinese medicines, rat pheochromocytoma pc 12 cells were used as a model to screen the neuroactive components from natural products. most of all, the effect of it on inducing the differentiation of pc 12 cells and / or execute the neuroprotective effect challenged by serum - deprivation and oxidative pressure, and its mechanism were studied in this paper
本文以利用我國豐富的天然藥物資源為目的,用大鼠嗜鉻神經瘤細胞pc12作為模型,篩選能模擬ngf的功能誘導pc12細胞分化、對神經細胞有一定保護作用的天然藥物,並對其作用機制進行初步探索。According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l
將菟絲子乾粉用75乙醇浸泡后,減壓蒸干后得到褐色漿狀物,經石油醚、氯仿、乙酸乙酯、正丁醇萃取,經柱層析后,再用葡聚糖凝膠對有效成份進一步純化,獲得了菟絲子中能誘導pc12細胞分化的活性組分。To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau
摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法,採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞,取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度;臺盼藍排斥試驗檢測活細胞數。After thymus transplantation, the embryo ' s thymus has trunk cell that comes from the embryo ' s yolk bag, embryo liver and marrow, it excretes a great deal of thymus hormone which change lymphocyte into mature t lymphocyte through multiplication and differentiation, then it help to turn b cell into serous cell, the quantity of the serous cell increases, the antibody that it produces also increases, that is the content of iga, igm and igg is increased
而移制胎兒胸腺后,由於胎兒胸腺內有來自胎兒卵黃囊、胚肝和骨髓的幹細胞,並能分泌大量胸腺素,促進淋巴細胞在該處增殖、分化為成熟t淋巴細胞,繼而輔助b細胞活化成為漿細胞,漿細胞數量增加,產生的抗體量增加,即igg , igm , iga含量增加。Bs of different dosages simultaneously inoculated rabbit with inactivated vaccines of rabbit hemorrhagic disease virus ( rhdv ). the hi liters of antibody against rdhv induced by rdhv inactivated vaccine in the group of high dosage bursin are higher than control group about 2 - 3 liters averagely. and the result of t - lymphocyte transformation assay shows that the bursin can temporarily enhance the t - lymphocyte transformation. the results of effects of bursin on body weight gain and feed conversion ration in rabbits show that bs can increase growth of mammal. the effect of bursin has a relation to its dosage, but has no ralation to the difference of interspecies and the method which come into the body
試驗中還發現, bs對雞體有一定的增重作用從而提高了飼料報酬。將不同劑量bs與兔瘟滅活苗同時免疫家兔,高劑量的dbs 、 cbs組可使兔瘟hi抗體水平較免疫對照組提高2 - 3個滴度。 t -淋巴細胞轉化試驗表明: bs可短暫促進t -淋巴細胞的活化。Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 ( hbmp - 3 )屬于tgf -超家族的一員,可以誘導間充質細胞分化為軟骨和骨,在胚胎時期骨骼發育和骨再生修復中起著重要的作用,而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用,因而hbmp - 3有廣闊的市場前景。它在人體內含量極微,盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達,但是由於原核表達系統缺乏翻譯后的加工修飾,真核表達系統存在成本高、產量低等特點,限制了其在臨床上的應用。There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ) i型膠原、纖維粘連蛋白促進細胞增殖,細胞接種后3 、 6 、 gd三個檢測時間點,實驗組細胞均明顯高於對照組。與1型膠原相比,纖維粘連蛋白刺激作用更強。 ) i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化,不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ,而且堿性磷酸酶活性明顯增高,堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性, i型膠原組mscs細胞堿性磷酸酶活性較fn組更高,有顯著性差異;同時,兔疫組化染色表明,經纖維粘連蛋白作用的mscs1型膠原表達陽性。Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗,確定本實驗室大鼠前體脂肪細胞的最佳培養條件是:溫度為37 ,濕度為95 , co _ 2濃度為5 , ph值為7 . 0 7 . 2 ,離心力為1000r / min ,培養基為m _ ( 199 )培養基,胎牛血清濃度為10 ,合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ,染色結果表明:油紅o染色是鑒定脂肪細胞的特異方法, gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少,觀察大鼠前體脂肪細胞分化過程中的形態變化,進而確定脂肪細胞的分化階段。Using the electric current cation and anoin exchange action, like l - ascorbic acid directly sends in the cell, the activation cell, the desalination pigment, the promotion collagen and the tension textile fiber regeneration increase skin organizes to guarantee the water ability, lets the skin tightly send smoothly has the elasticity, achieves the crystal clear beautiful skin effect
利用電流正負離子的交換作用,如左旋c直接送入細胞中,活化細胞、淡化色素、促進膠原蛋白及彈力纖維的再生增加皮膚組織的保水能力,讓皮膚緊致光滑有彈性,達到晶瑩剔透的美膚效果。Increased skin surface activity, the metabolism of trace elements regulate cell growth. enhance the vitality of elastin and collagen protein, preventing cell aging and promote cell regeneration and increase blood circulation. improving metabolism, anti - bacterial, anti - itching … … the aging of the skin, relax, bark, diaopi, have had some effect
增加皮膚表面酶活性,調節細胞生長新陳代謝所需的多種微量元素,提高彈性蛋白與膠原蛋白的活力,具有防止細胞老化,促進細胞再生,增加血液循環,提高新陳代謝,抗菌,止癢… …對皮膚老化,鬆弛,死皮,掉皮,有一定作用。He came through a cell, evolved through a cell. how did he evolve
人類是由細胞進化而成,但人類怎樣進化,進化有甚麼目的?Interviewer : the theory that scientists say that man came from a little cell from heaven knows where. how did you feel about those
問科學家的理論說人類是由一個最原始的細胞進化而成,你如何將這個理論結合到你所說的事情?分享友人