細菌的轉化 的英文怎麼說
中文拼音 [xìjūndezhuǎnhuà]
細菌的轉化
英文
bacterial transformation-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control
選取單個菌落分別點種到刪平板和md平板,找出在回d上生長正常, w上生長緩慢或不生長的菌落,即陽性菌落,再以陽性菌落分別塗布zeocine含量500ug加, 1000ug砌l的ypd平板,以高濃度的抗生素篩選高拷貝的酵母工程菌,在含500ug ml高濃度抗生素平板上獲得了15個轉化子,取其中5個進行pcr擴增,有3個擴增產物與陽性對照相同,說明此酵母細胞中已含有s hbsag融合片段,其中之一命名為pSulfid also can be regarded as a marker of the action of sulfur bacteria. 8 ) based on research results, author postulated that early generation of hydrocarbons is closely related to the action of sulfur bacteria. many kind of algae such as dinoflagellates, diatom, prynesiophytes etc have rich biological lipids which has lower polymerization
6 、從未熟一低熟源巖生烴組分及其演化、可溶有機質轉化生烴等方面,探討了未熟一低熟油的形成機制,提出本區未熟一低熟油氣的形成是低活化能的富氫腐泥組分受到硫細菌早期低溫降解作用的結果。A new transgenic system of gerbera mediated by agrobacterium tumefaciens
小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系Washed air purifier working principle : siphon and using centrifugal principle will be mixed in water pure plant essential oils inhaled through its siphon principle the motor base coaxial centrifugal turbines in the bottom of straw through exchanges cover a very high - speed rotary motor, reuse centrifugal principle, will be mixed in water pure plant essential oil spray bottle in the form within a water film bile, the dust in the air and inhaled bacteria in water purification at the same time after the indoor air insufflation, quickly and efficiently by removing indoor toxin biological, dust, cigarette smoke, the smell, virus
水洗空氣清新機工作原理:是利用虹吸以及離心原理;將混合於水的純植物精油通過虹吸原理吸入其電機底座的同軸離心渦輪下部的吸管中,通過交流罩極電機高速旋轉,再利用離心原理,將混合於水的純植物精油噴在瓶膽內形成一層水膜,將空氣中的灰塵以及細菌吸入水中,同時將經過凈化的空氣吹入室內,快速有效地去除室內的有毒素生物、灰塵、煙味、臭味、病毒等。Inhibitors of cyp51, such as azalanstat ( rs - 2i607 ) and rs - 2i745, could inhibit the synthesis of ff - mas to decrease the accumulation of ff - mas. inhibitor of a14 - reductase, such as ay9944 - a - 7, could inhibit the metabolism of ff - mas to increase the accumulation of ff - mas ; and some other reagents, such as nystatin, could combine with the downstream intermediate in the cholesterol biosynthesis pathway to accumulate mas. in this study, we investigated the role of mas by using these reagents to change the level of endogenous ff - mas
抑制cyp51酶的抑制劑,如azalanstat ( rs - 21607 )和rs - 21745等,均能抑制mas的合成,降低mas的積累;而抑制14 -還原酶的抑制劑,如ay9944 - a - 7等,能抑制ff - mas向t - mas的轉化而造成ff - mas的積累;還有一些物質,如制黴菌素,能阻止mas向下游的代謝而造成其積累,本文主要通過應用這些物質降低、或增加組織細胞中內源性mas的積累,來研究mas的作用。In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein
在胃裡,這種酸的作用是殺司食品的細菌,使食物軟化,把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后,通過電擊轉化整合p . pastorisgs115菌株細胞中。The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21
本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點,成功構建重組表達質粒prt - p450nor和pet - p450nor ,並轉化到e . colibl21 。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。The results showed that quantity of bacterium and four bacterial physiology groups was positively correlated with quality of illumination ; their quantity showed a reduced tendency with the reducing of the illumination condition, but quantity of fungi was negatively correlated, it was increased gradually with the reducing of the illumination condition ; rhizosphere soil of kentucky bluegrass turned into fungi type from bacterium type ; the rhizosphere effect of various bacterial physiological group of kentucky bluegrass is obvious under different quality of illumination
結果表明,草地早熟禾根際細菌及四類細菌生理群數量與光照條件呈正相關,隨著光照條件的減弱,其數量呈降低趨勢;根際放線菌數量隨光照的減弱呈先下降後上升的趨勢;而真菌數量與光照條件呈負相關,隨著光照條件的減弱,根際真菌的數量逐漸增加;草地早熟禾根際土壤由「細菌型」向「真菌型」轉化;不同光照條件下,根際各微生物類群都表現出明顯的根際效應。This paper discusses how the silicate bacteria affect potassium releasing from minerals, especially the function mechanism during the interaction between bacterial and minerals ; the paper emphasize the problem such as the utilization of silicate bacteria to release significant amounts of potassium from soil minerals in the karst area, and at the same time the utilization of the silicate bacteria in the agriculture of karst area is discussed
主要探討矽酸鹽細菌的解鉀作用,以及使難溶性礦物態鉀轉化為速效性鉀的作用機理;同時在研究矽酸鹽細菌解鉀作用機理問題的基礎上,重點探討了喀斯特環境中利用矽酸鹽細菌活化土壤中的礦物鉀元素的問題,特別是矽酸鹽細菌在喀斯特環境中農業上的利用。Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
( 3 )細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣,細胞核轉化獲得了生長良好的抗性萵苣植株,經pcr 、 pcr - southern 、 southern斑點雜交分析證實, adw基因已整合到萵苣基因組中。We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going
構建了用於水稻中表達的載體pca - ced9 ,通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞,經潮黴素( hym )篩選,以期望獲得抗性植株,目前該工作仍在進行中。New minimally heated refrigerated foods are raising concerns about the hazard, he will tell delegates. other topics include how the complete genetic blueprints or genomes of food - poisoning bacteria such as campylobacter are transforming the science of food safety, and the latest research into potentially deadly strains of the e. coli gut bacterium
本次研討會的其它議題還包括:諸如彎曲桿菌這類食源性有毒細菌的完整基因圖譜或基因組圖譜是如何轉化成為食品安全科技的,以及對大腸桿菌這種腸內細菌有可能轉變成致命菌株的最新研究成果。In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules
研究發現呷基因的調控區存在多種轉錄因子結合位點,功能主要涉及組織特異性表達調控,細胞生長發育相關基因的表達調控,刺激反應基因的表達調控等; ndrgz蛋白在結構上屬于a小水解酶類折疊,折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似,提示ndrgz蛋白具有一定酶活性,可能參與細胞抗氧化應激反應,維持細, an ) armtbffiofbfochmilsyn ) mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡,參與內外源有毒物質的代謝等。Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切,電泳回收目的片段,將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中,轉化大腸桿菌tgi感受態細胞,得到的轉化子經pcr鑒定和酶切分析,篩選出符合閱讀框的重組子,構建成重組表達質粒pet - hn ,並在大腸桿菌bl _ ( 21 ) ( de _ 3 )宿主菌中成功地表達了含目的蛋白的融合蛋白,融合蛋白的分子量約66kd ,加入iptg誘導8h后,蛋白表達幾乎達到最高水平。Some transcriptional factors may be expressed to regulate the expression of other genes to resist to antibiotics
通過一些與轉錄有關一些因子的表達變化來調節細菌的基因表達。The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert
經瓊脂糖電泳檢測,將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中,再轉化大腸桿菌tgi感受態細胞,得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。How the complete genetic blueprints or genomes of food - poisoning bacteria such as campylobacter are transforming the science of food safety
諸如彎曲桿菌這類食源性有毒細菌的完整基因圖譜或基因組圖譜是如何轉化成食品安全科技的。分享友人