組分篩選 的英文怎麼說

中文拼音 [zǔfēnshāixuǎn]
組分篩選 英文
component screening
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation

    簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。
  3. Methods : we have divided the 636 molars ( without dental caries or pathological changes of root ) collected in school of forensic medicine and stomatological hospital in shanxi medicine university into four groups : maxl, max2, manl, man2, and selected 5 indexes closely related to changes of dental age ( dental attrition, contact area, the index of dentine marrow cavity, the thickness of cementum of root, the diaphaneity of dentine of root ), and proposed the grading standard and scoring standard date processing and statistical analysis after measuring the teeth of the four groups

    方法：從山西醫科大學法醫學院及口腔醫院收集的636磨牙（無齲壞、無根尖病變）分為max1 、 max2 、 man1 、 man2四組，根據牙齒的增齡變化特點，篩選了5個與牙齡變化密切相關的指標（牙齒的磨耗、接觸區面積、牙本質髓室指數、根尖牙骨質的厚度、根尖牙本質透明） ，提出了指標的分級標準和評分標準，對各組的牙齒測量后進行數據處理和統計分析。
  4. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  5. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Methods the optimal proportion of levant storax oil and dalbergiae odoriferae oil, which were active ingredients of the traditional chinese medicine styrax and dalbergiae odoriferae, was bolted by using uniform - design method ; the myocardial ischemia model of rats was set up through the induction of isoprel and the above rats were divided into 5 groups, which were model control group, high dose group of shuangxiangyou, middle dose group of shuangxiangyou, low dose group of shuangxiangyou, and positive control group of muskone

    方法傳統中藥蘇合香和降香的有效成分蘇合香油和降香油最佳配比採用均勻設計方法篩選；建立由異丙腎上腺素造成的心肌缺血大鼠模型，將實驗分為模型對照組、雙香油高、中、低3個劑量組及冠心蘇合丸陽性藥物對照組后進行藥效學實驗。
  8. Selection maskers are the important ingredient of the vector, and it is also a hot research spot of the genetics of lactic acid bacteria at present

    篩選標記是載體的重要組成部分，也是目前乳酸菌遺傳學研究領域的一個熱點。
  9. The tl seeds are screening on the ms medium which contains 50ug / ml kan, and there are about 70 % of the transgenic lines showed the kan - resistance with a ratio of 3 : 1. however, the other lines did n ' t show the ratio consistent with the mendelism. it might prove that most of the transgenic plants only have one insert

    對收獲的轉基因番茄t _ 1代種子進行卡那黴素抗性篩選，結果發現， 70以上獨立轉化株系的t _ 1代種子出現了3 1的抗性分離，另一些獨立轉化株系的種子並未表現出符合孟德爾遺傳的分離比，說明大部分er - shsp基因是以單拷貝的方式插入植物基因組。
  10. Note : 1 ) p = prefectural ; n = national. 2 ) hc = high combining ability ; sr = strong restoring ability ; gq = good quality ; rb = resistant to biast ; psr = persistent resistance of blast ; ct = cold tolerance ; hoc = high outcrossing rate ; hsy = high and stable yield ; wa = wide abaptability ; geq = good eating quality ; bp = big panicle ; hy = high yield ; mrb = moderate resistance of blast

    武陵山區國家水稻品種區試有必要加快分熟組的步伐，同時建立專門的抗（耐）寒性鑒定基地，以期篩選出適應性更廣，安全性更好的強優新組合。
  11. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  12. Conclusion : the system not only could pick upon the op timum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor z ymogen upgrowth in fermentor and determine total number of bacteria in fresh cre amery rapidly

    結論：研製的生物傳感器系統可用於菌種和培養基組分的快速篩選、微生物活細胞生長特性的快速評估，還可用於發酵罐中發酵菌的生長監測及新鮮牛奶中雜菌總數的快速測定。
  13. 281 double crossover mutants were examined by fermentation experiments and hplc analysis for production of polyketides. besides avermectin b1a and b2a, a new compound ( about l ~ 4ug / ml ) which showed a retention time and fragmentation patterns identical to those of the authentic sample of 22, 23 - dihydroavermectin bla by hplc and lc / ms analysis were detected in culture extracts of 27 isolates

    在篩選的281個雙交換突變株中，有27株除了產生阿維菌素b1a和b2a ，還產生一個新組分，經hplc和lc ms分析，證實該組分為c - 22 ， 23雙氫阿維菌素b1a （即伊維菌素b1a ） 。
  14. The total amount of avermectins b were only about 0. 5 ~ 2 % of those produced by the parental strain olm73 - 12. this study suggests that t

    在281個突變株中，還篩選到了2個突變株，其發酵產物幾乎全部為有效組分阿維菌素b1 ， b2組分僅佔5 。
  15. The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease

    主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。
  16. Metallophalocyanines ( mpcs ) are a kind of centrosymmetric planar organo - metallic molecules with an extensively delocalized two dimensional conjugated - electron system which show a relatively large third order optical nonlmearity, varying upon central metal atom substitution and other factors. other interesting properties of this molecule and many of its derivative products are their versatility, architectural flexibility and high environmental stability, which are very important requirements to implement photo - electronic applications

    因其骨架結構特徵和可通過選擇中心離子、軸向配體和在酞菁環上引入功能性取代基等方法進行分子篩選與組裝得到具有特殊的物理化學性質和光、電、催化等功能的材料，而引起化學家和材料學家的濃厚興趣。
  17. And the differentiation of bmscs were determined to be chondrocyte after g4i8 selection. the bmscs in controlled group were dead about 1 week after g4, 8 selection. conclusions : 1

    對照組bmscs轉染后增殖活性較實驗組低，沒有向軟骨細胞分化的趨勢，經g _ （ 418 ）篩選后1周左右全部死亡。
  18. In the present study, a compartment cultivation system and histochemical staining were used to investigate the influence of soil available p level, plant p status and soil organic p on the growth and metabolic activity of am fungi. differences in metabolic activity among am fungal isolates and the relationship between metabolic activity and mycorrhizal effectiveness were al so investigated. in addition, am fungi from a wide range of environmental conditions ( originally isolated from north, central and south china ) were used to study the ecological adaptability of am fungi and the influence of edaphic conditions on am fungal growth and metabolic activity

    本研究採用分室根箱、組織化學等手段研究了土壤施磷水平、植物磷營養狀況、土壤有機磷對am真菌生長和代謝活性的影響；不同am真菌的代謝活性及其與菌根效應之間的關系，並對我國華北、華中和華南地區篩選出的高效菌株進行了生態適應性的比較，以期在理論上闡述宿主植物生長狀況及土壤條件對菌根真菌生長和代謝活性的調控機制，篩選出具有廣泛生態適應性的am菌株。
  19. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  20. Secondly, introducing the image analyzing technology with reference to the disadvantages of the traditional ferr - graph analysis technology, and with the combination of characteristic parameter optimizing filtration so as to raise a description method of debris micro - morphologic character. thirdly, with the application of mode recognition method, completing the process of debris auto - recognition based on the collected information of the debris configuration characteristics ; and conducting the diagnosis on the aero - engine wear faults according to the theory of particle tribology. fourthly, introducing information fusion technology to solve the problem that a single method can not collect enough fault premonitory information to conduct the wear fault diagnosis, hence to conduct the research and exploration in the field of comprehensive diagnosis on the aero - engine ' s multi - fault premonitory information

    本文的研究工作主要包括以下五個部分：首先，介紹航空發動機常見的磨損故障類型，研究磨損故障的失效機理，分析磨粒的產生機理、分類以及形態特徵：其次，針對傳統鐵譜分析技術的缺點，引入圖像分析技術，再結合特徵參數優化篩選，形成基於圖像的磨粒顯微形態學特徵描述方法：然後，基於提取到的磨粒形態特徵信息，應用模式識別方法完成磨粒自動識別，並根據顆粒摩擦學的基本原理進行航空發動機磨損故障的診斷與定位：再后，鑒于單一方法不能提取足夠的故障徵兆信息進行磨損故障診斷，本文引入信息融合技術，開展航空發動機多故障徵兆信息綜合診斷方法的研究與探索；最後，基於航空發動機滑油光譜分析與鐵譜分析數據，應用時序模型、灰色模型以及組合模型進行磨損故障的預測方法研究。
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