組特異性引物 的英文怎麼說

中文拼音 [zǔtèyìxìngyǐnwù]
組特異性引物 英文
group-specific primers
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 特異性 : distinction
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. And understanding and studying the spectral features and variation rules of geo - targets in the experimental area, raising that it is the basis of geo - targets information collection with imaging spectrometer data to understand spectral features and variation rules of geo - targets, realizing that in a great extent spectral - integrated - form - based classification method can remove the phenomenon of " different spectrum with same objects " resulted from reflection ratio curve translation because of the angle change among sensor, targets and observation direction, and the average and variance images can be introduced to solve the problem of two kinds of geo - target with similar spectral forms and much different values of whole reflection ratio. it is suggested that " red edge " range bands of vegetation, which has close relationship with vegetation cover and biomass, is the main characteristic bands and important basis for careful vegetation classification and quantitative retrieval, and pixel - based derivative spectral analysis is very useful for removing the effects of soil background values and quantitatively retrieving vegetation biomass and cover. the remote sense quantitative retrieval model is developed for main appraisable factors of desertification monitoring assessment with imaging spectrometer data and then the applicability of model is analyzed

    研究結果如下：首先針對荒漠化地區的地物特徵，對高光譜數據不同波段的數據質量、波段組合進行了評價，提出了適用於荒漠化監測的基本波段選擇集；初步了解和掌握了研究地區的地物光譜特性及變異規律，進一步明確了掌握地物光譜特徵和變異規律是用成像光譜儀數據提取地物信息的基礎；發現了基於光譜整體形狀的分類方法在很大程度上能夠消除由於傳感器、地物目標觀測方向之間的角度變化引起的反射率曲線整體平移的「同物異譜」現象，對于譜形相似而整體反射率的值相差較大的兩類地物，通過引入均值和方差圖像參與分類得到解決；研究還表明在植被「紅邊」范圍內的波段是進行荒漠化監測的主要特徵波段，這些波段與植被生物量和蓋度都有密切的關系，是開展精細植被分類研究和植被定量反演的重要基礎；像元的導數光譜分析可以消除土壤背景的影響，是進行植被生物量和蓋度定量反演的有力工具；建立了荒漠化監測主要評價因子的定量反演模型，並分析了模型的適用性。
  3. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  4. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。
  5. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  6. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  7. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  8. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  9. This paper unfolds engineering disposition and theoretical research of negative temperature high performance concrete ( nthpc ) with the damage of many factors motivation, the project background based on qing - zang railway engineering, which is one of four - emphasized engineering during the tenth five - plan. due to the difference of environment of construction and service between nthpc and ordinary concrete, and therefore durability of nthpc is required to higher level. at a first, nthpc must avoid frozen damage at early age and possess anti - freezing property at later period, in order to meet engineering practicable application need, and this are two emphasized and difficult problems, as for nthpc ; by means of mechanism analysis about deicing - agent ingredient and anti - freezing at early period, adopting composite technique routine of mineral addition + anti - freezing element + water - reducer4 - air - entraining + anti - erosion of steel component etc, based on orthogonal experimental approach, fd - 1 composite functional admixtu re was manufactured, which has more property and orientation on qing - zang railway

    負溫混凝土由於和普通混凝土在施工環境及服役環境上存在的差異，因此表現為比普通混凝土更為較高的耐久性要求；負溫混凝土首先要避免早期的凍害以及具備長期抗凍性能，才能夠滿足工程實際應用的要求，這也是負溫混凝土必須解決的兩大技術關鍵；通過對目前常用防凍劑組分作用機理的分析研究及混凝土早期防凍機理探討，採用礦物外加劑+防凍組分+高效減水劑+引氣+阻銹組分功能復合的技術路線，通過正交試驗設計復配了適應青藏鐵路工程要求的專用多功能復合型外加劑fd - 1 ；並在此基礎上配製不同等級負溫高性能混凝土，開展一系列包括硫酸鹽侵蝕、氯離子滲透、抗凍融循環、收縮及耐磨性等耐久性能研究；通過對fd - 1組分和摻量的調整，優化負溫混凝土在施工特性、力學指標和耐久性三個方面的兼容、協調性。
  10. 300 clear bands including 289 polymorphic bands and 50 specific bands were amplified from the genomic dna of gastrodia elata bl. by these selected primers. the rate of polymorphic bands and the rate of specific bands were 98. 0 % and 16. 6 % respectively

    6個引物組合共擴增出300條清晰可辨的條帶，其中289條多態性條帶， 50條特異性條帶；多態性條帶率和特異性條帶率分別達98
  11. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  12. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  13. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  14. The results shows that the community richness, species diversity indices and evenness of tree layer and shrub layer share the same order, of which is higher in pure limestone region than that of in pure dolomite region, but it reverse in herb layer ; the coefficient of similarity is considerably low between all plots, while species turnover rate is quite high among the plots and is higher in dolomite region than that of in limestone ; in different karst regions, karst processing leads to niches diversity characterized by special morphologies and special element geochamical features, and therefore affect the dynamic and features of plant communities

    我們在最有代表性之一的貴州茂蘭喀斯特森林保護區選取純灰巖和純白雲巖兩種巖性的喀斯特環境，對其元素地球化學特徵及其中原生性植物群落的相異性和物種多樣性進行了對比，結果發現：不同巖性區域多樣性：喬木層和灌木層為純灰巖區純白雲巖區，草本層為純白雲巖區純灰巖區，總體上純灰巖區的高於純白雲巖區的；各樣地間的相似性系數都很低，相同巖性類型內的明顯高於不同巖性類型之間的相似度；物種周轉率高，且白雲巖區高於石灰巖區，種類組成差異明顯；喀斯特區可溶巖地球化學背景通過喀斯特作用導致特殊的地形條件與元素地球化學特徵，並引起局部小生境的分異從而影響植物群落特徵。
  15. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  16. The hemaglutinin ( ha ) of aiv plays the key role in determing the pathogenicity, cell receptor binding property and host range of the virus. the homology of the ha sequences reported and registered in genbank of different strains of h _ ( 5 ). h _ ( 9 ) subtype was respectively analyzed and compared with each other. the conservative domin of ha seqence of h _ ( 5 ), h _ ( 9 ) subtype was selected for pcr amplification. two sets of specific primers were designed

    本研究根據genbank中查出的禽流感病毒h _ 5亞型，禽流感病毒h _ 9亞型的ha片段基因組序列，利用dnasis軟體分別對aiv的h _ 5 、 h _ 9亞型ha基因區域進行同源性比較，設計篩選出兩對聯合pcr反應的特異性引物，其中一對是h _ 9亞型通用引物，擴增出ha片段695bp ，另一對引物為h _ 5亞型的通用引物，擴增出的ha區域部分目的片段約為448bp 。
  17. The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively

    Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上，結合已發表的文章及基因序列，針對senv - d和senv - h基因組設計特異性引物，利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株（ senv - d - bj1 ）的全部編碼區基因序列，還得到了一個senv - d亞型分離株（ senv - d - bj2 ）的大部分編碼區基因序列（包括orf1和orf2 ） ；從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株（ senv - h12 - 1和senv - h12 - 2 ）的全部編碼區基因序列。
  18. In abroad, the study of integration site used for transgenic detection had just begun. in this study, according to the collection of the global commercialized transgenic crops, select seven exogenous genes which basically cover the total commercialized crops, namely camv35s and fmv promoter, nos terminater, mark gene nptii, and aim genes pat, epsps and cryia ( b ). use endogenous 18srrna gene as collate, design a large pairs of specific primers, screen the optimum primers groups, optimized the test condition and parameters, establishing the qualitative pcr detection system

    本研究根據收集的國內外已商品化的轉基因作物品種，選擇了能基本覆蓋商品化轉基因品種的7個外源基因，即： camv35s 、 fmv啟動子、 nos終止子、 npt標記基因和目的基因pat 、 epsps 、 cryia （ b ）作為篩選目標，以植物18srrna基因作為內源參照基因，設計了多對特異性引物，並篩選出最佳組合，優化了檢測條件和參數，建立了pcr定性檢測方法體系。
  19. These conventional approaches cannot be selectively targeted to the tumor cells and completely eradicate them, this may account for the development of recurrence and metastasis of gastric cancer due to the existence of residual tumor cells ; in addition, as to some kinds of conventional approaches, such as chemical therapy, their tumoricidal effects also could cause damage to some other normal tissues / cells and undermine the function of immune system in patients with gastric cancer

    目前，臨床上的?些常規方法，包括外科手術的、化學的及物理的治療手段，對絕大多數中晚期胃癌的療效均不理想。其原因在於這些常規方法對癌細胞缺乏選擇性，並不能特異性地殺傷癌細胞，勢必造成殘存癌細胞引起的腫瘤復發和轉移。某些常規方法，如化療，在殺傷癌細胞的同時，也會造成機體正常組織的損傷並削弱機體的免疫機能。
  20. At first, total 31 transcription factors correlated to cell proliferation, differentiation, physiological stress and apoptosis, were selected ( see in article ) to use in the two - hybrid, and cloned into plasmid pact by using rt - pcr to amplify these transcription factors from fetal tissue cdna or stimulated lymphocyte cdna. as result, only six transcription factors atf3, atf4, e2f6, c - jun, c - fos and p53 were correctly cloned

    基於以上的背景信息，本文利用細胞雙雜交技術，對resrin在細胞周期中可能的作用的轉錄因子進行了篩選:一、首先從genebank中篩選了31個與細胞周期密切相關的轉錄因子，設計了特異性引物。從胎兒組織和刺激后的淋巴細胞中克隆了這些轉錄因子並構建於細胞雙雜交系統的pact質粒中。
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