組織化學法 的英文怎麼說
中文拼音 [zǔzhīhuàxuéfǎ]
組織化學法
英文
histochemical method- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 織 : 動詞(編織) knit; weave
- 學 : Ⅰ動詞1 (學習) study; learn 2 (模仿) imitate; mimic Ⅱ名詞1 (學問) learning; knowledge 2 (學...
- 法 : Ⅰ名詞1 (由國家制定或認可的行為規則的總稱) law 2 (方法; 方式) way; method; mode; means 3 (標...
- 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
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Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1
我們以小鼠為模型,運用組織化學、免疫熒光組織(細胞)化學、流式細胞儀分選方法( facs )以及分子生物學手段,研究了小鼠乳腺的發育規律:小鼠乳腺組織中類乳腺幹細胞:小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定;小鼠類乳腺幹細胞分化的潛能;小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明: 1The method of assay for generation of active oxygen species includes spectrophotometry, fluororesence, chemiluminescence, histochemistry, etc
活性氧的檢測方法主要有:分光光度法、熒光法、化學發光法、組織化學法、金屬沉澱法等。Methods the influence of fluoride on the rat first passage osteoblast were evaluated by histochemistry, enzymehistochemistry and electron microanalysis in vitro
方法應用組織化學、酶組織化學和電子顯微分析等手段觀察氟對體外培養的鼠第一代成骨細胞的影晌。The distribution of nitric oxide synthase ( nos ) in the rhabdophis tigrinus brain was studied by using reduced nicotinamide - adenine - dinucleotide phosphate ( nadph ) - diaphorase histochemistry
摘要運用nadph - d組織化學方法,對虎斑頸槽蛇腦的一氧化氮合酶進行了定位研究。In order to investigate the ontogeny of the pituitary prolactin ( prl ) expression of sheep fetuses during gestation, the pituitary glands were collected at days 60, 90, 120 of gestation, the first day after birth, respectively. the cells containing the prolactin ( prl ) were detected using immunohis ' ochemistry method
為了研究綿羊胎兒垂體中催乳素細胞( lactotrophs )的發育和功能變化,分別在胎兒發育的第60 、 90 、 120天和出生期採集垂體,利用免疫組織化學方法研究了垂體中催乳素( prolactin , prl )的表達及其變化。Oogenesis was described briefly and the ways of the formation of yolk granules were explored. at first, eight stages in the oogenesis of the acrida chinensis were distinguished and described respectively, basing on the changes of the oocyte ' s size, chromosomal changes in the nucleus of the oocyte, vitellogenesis and the size of the follicle cells
在基礎理論方面,本研究首先在光鏡水平對中華蚱蜢卵子發生進行了階段劃分;並以此為依據,用組織化學方法對卵子發生中卵母細胞與濾泡細胞遺傳物質的變化及作用,組成卵黃的生物大分子物質的生成及變化進行了初步研究。Objectie. we ealuated the effects of intradiscal administration of substance p - saporin, a toxin selectie for cells expressing substance p receptors
研究設計.應用免疫組織化學和逆行神經示蹤方法,檢查支配鼠腰椎間盤的背根神經節細胞中p物質受體的變化。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。We investigated the expression changes of islet - 1 in the adenohypophysis of sheep fetuses during gestation from 60 day to newborn by immunohistochemistry
本研究用免疫組織化學方法檢測了islet - 1在綿羊胎兒腺垂體細胞中的表達及其變化。Methods : the expression of pcna and fibronectin in placenta of 30 patients with early abortion were detected by immunohistochemistry
方法:通過免疫組織化學方法觀察30例早期自然流產患者胎盤中增殖細胞核抗原、纖粘連蛋白的表達。The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation. 2. following lps or seb was administered intraperitoneally, the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice. double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ,用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達,並採用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。The relationship between the relative contents of 5 - hydroxytryptamime ( 5 - ht ) and its receptor and age in days of postnatal rat was studied by using immunohistchemistry and in situ quantitative analysis
為研究大鼠頜下腺5 -羥色胺及其受體的相對含量與日齡的關系,用免疫組織化學法結合原位定量,測定了大鼠頜下腺5 -羥色胺及其受體免疫反應產物的相對含量。The permeation of mps into superficial tissue was demonstrated using a radioactive labeled substance as well as chemical - analytical and histochemical methods
用放射活性標記物質、化學分析和組織化學法證實mps (多磺酸粘多糖)能滲透入淺表組織。Immunohistochemistry staining showed that induced different - passage mscs expressed neurofilament ( nf ) and neuron - specific enolase ( nse ). special nissl bodies were obvious in the neuron - like cells by histochemistry staining
免疫組化法檢測發現這些神經細胞強表達神經絲蛋白( neurofilament , nf )和神經元特異性烯醇化酶( neuron - specificenolase , nse ) :組織化學法檢測可見神經元特有結構尼氏體。Abstract : estrogen receptor ( er ) and androgen receptor ( ar ) assays were performed with fluorescent histochemical method in 89 cases of bladder carcinoma. 77 cases were followed up for at leazt six years. the tumor recurrence occurred in 32 ( 41. 56 % )
文摘:採用熒光組織化學法測定了89例膀胱移行細胞癌雌雄激素受體的表達,獲長期隨訪的77例中有32例復發,復發率為41 . 56 % 。Methods paraffin method, semi - thin sectioning, and histochemical method were used
方法石蠟切片法、半薄切片法、組織化學法。Laboratory animal - immunohistochemistry
實驗動物免疫酶組織化學法Methods : immunohistochemistry methods were used
方法:免疫組織化學法。Passage 2, 5 and 8 of umbilical cord blood mscs were induced to differentiate into neuroncytes. the results indicated that about 70 % of cells exhibited typical neuron - like phenotype. immunohistochemistry staining revealed that induced different - passage mscs expressed nf and nse ; special nissl body was obvious in the neuron - like cells by histochemistry staining
取擴增第2 、 5和8代的msc分別向神經細胞誘導,誘導后的細胞平均有70左右呈現典型的神經元表型,免疫組化法檢測發現不同代數的msc誘導后均表達nf和nse ;組織化學法檢測可見神經元特有結構尼氏體。分享友人