結構菌核菌 的英文怎麼說

中文拼音 [jiēgòujūnjūn]
結構菌核菌 英文
tubercullnic acid
  • : 結動詞(長出果實或種子) bear (fruit); form (seed)
  • : Ⅰ動詞1 (構造; 組合) construct; form; compose 2 (結成) fabricate; make up 3 (建造; 架屋) bui...
  • : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
  • : 核構詞成分。
  • 結構 : 1 (各組成部分的搭配形式) structure; composition; construction; formation; constitution; fabric;...
  1. At the earliest developmental stage of the myxomycetes, the un - nucleate amoeboid or swarm cells of all the species are similar to each other in the aspects of morphology, structure and habit

    在孢子萌發后的最初發育階段中,所有黏的單黏變形體和游動胞在形態、和習性等方面均相似。
  2. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    論:截短的乙型肝炎表面抗原分子的原和真表達』重組質粒成功被建及分別在人腸桿efl得到誘導表達和存貞細胞ifj表達,並檢測劍其表達產物的抗原特性。
  3. In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm

    發現noea與中慢生根瘤( mesorhizobiumsp . ) bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ,而其303 - 362區域與大腸桿( escherichiacoli )的糖體50s亞基的l11蛋白甲基轉移酶( prma )的160 - 220域的相似性達到41 。
  4. Effect of ice nucleation active bacteria on the ultrastructure of apricot varieties ovule

    對仁用杏胚珠超微的影響
  5. Ultrastructure of nucleus and sclerotium of fuligo septica phanero plasmodium

    顯型原質團細胞的超微
  6. The pathogenic strains of yersinia enterocolitica carry a 45 - kb high pathogenicity island ( hpi ) comprising iron - uptake - related genes such as irp1 - irp2 and fyua, which has been observed in 93 % of the 60 entero - adhesive e. coli { eaec } strains and 80 % of the e. coli isolated from blood samples

    Hpi毒力島功能心區主要的基因為irp2 、 irp1和fyua ,是高度保守的。 irp2和irp1分別編碼兩種高分子量蛋白hmwp2和hmwp1 , fyua編碼鼠疫素受體。
  7. A fungus ball composed of blue - staining hyphal elements of aspergillus is seen here in a bronchus. fungus balls may also form when fungi colonize cavitary lesions of tuberculosis

    支氣管處可見由藍色的成的真球。當真在因損害形成的腔洞內植入時,就可能形成真球。
  8. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術,將其蛋白vp2基因亞克隆至原表達載體pproex - htb , iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白,光密度掃描對表達產物進行初步定量,表明表達產物約占體總蛋白的14 。
  9. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的建蛇毒鋸鱗蝰素( echistatin )的原高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,合大腸桿蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術建echistatin的融合表達基因克隆。
  10. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的:以il - 12作為分子佐劑,與新抗原mtb8 . 4基因連接形成嵌合分子,將其克隆到真表達質粒中,建成嵌合dna疫苗,研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用,為尋求安全、有效、廉價的病新疫苗打下基礎。
  11. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿表達載體pproex ~ ( tm ) ht中,建重組表達質粒並進行確證性序列測定,重組質粒測序果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原表達載體pproex ~ ( tm ) ht的目的位點。重組質粒轉化受體dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合蛋白分子量約為18kda ,表達的融合蛋白經薄層掃描發現目的蛋白表達量約占體蛋白的30 。
  12. Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody. our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4. after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4. our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells

    我們建的rhpn原高效表達系統經m page及凝膠密度掃描分析果表明, rhpf4表達量占體總蛋白量的25 30 ,較原表達克隆pt7 7 rhpf4提高了近80倍,經快速高效的包涵體分高純化工藝和復性工藝, rhpf4具有野生蛋白活性。
  13. Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

    運用goldenkey分子生物學軟體對prrsvbj - 4蛋白的抗原表位及其二級進行了分析和比較,從中篩選13段顯示表位特徵的氨基酸殘基序列,用pcr技術擴增相應的苷酸片段,將其插入到噬體表達載體m13ke ,果預測的13個表位可在噬體表面得以展示。
  14. Isolation and purification of four main peptide toxins from a. fuligiea were undergone by means of rp - hplc, and idenfication of the chemical structure of - amanitin and - amanitin by nuclear magnetic resonance ( nmr ) showed that the the chemical structure were the same as the reference

    對灰花紋鵝膏的四種主要肽類毒素進行了分離純化,並用質譜和磁共振對兩種鵝膏毒肽( - amanitin 、 - amanitin )進行了鑒定。果與文獻報道的相一致。
  15. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象,選用真表達質粒pci - neo ,根據已建好的含54位密碼子突變型mbl基因t載體的,設計合成新的引物, pcr擴增54位密碼突變型mbl基因,凝膠回收,雙酶切pcr產物和pci - neo質粒, t4連接酶連接,將前者克隆至後者的sma和sal位點,轉化大腸桿xl - 1blue ,氨芐選擇培養。
  16. The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area, which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis

    而16srdna克隆文庫分析和dna - dna雜交的果表明「」區沉積物中這兩類細數目很少,說明「暖池」區沉積物中的微生物群落特徵是對環境因素的一種響應,同時也可能是影響該海區深海及海洋環境的一個重要因素。
  17. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法: pcr擴增小鼠pemcdna編碼序列,將它克隆到含有gst的原表達質粒載體pgex - 4t - 3上,轉化大腸桿bl21 ,誘導表達gst mpem融合蛋白,通過親和層析,獲得初步純化的產物,以羥胺切割驗證其一級
  18. And also of increasing concern to health professionals and organizations worldwide is the sharp rise in drug - resistant strains of tb

    同時,耐藥株的迅速出現引起全球健康專業人員和機越來越多的關注。
  19. Many, though not all, terrestrial prokaryotes ( simple one - celled organisms such as bacteria that lack a membrane - bound nucleus ) and eukaryotes ( organisms with well - defined nuclei ) could survive this temperature range

    地球上許多原生物(簡單的單細胞生物,沒有細胞,如細)與真生物(有清楚細胞的生物)都能存活於前述的溫度?圍內。
  20. Whether they are more closely related to the eukaryotes than to bacteria is hotly debated

    它們糖體的形狀和和真生物的糖體類似程度高於和細糖體的類似程度。
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