結腸結核 的英文怎麼說

中文拼音 [jiēchángjiēhé]
結腸結核 英文
tuberculosis of colon
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 腸 : 名詞1. （消化器官的一部分, 通稱腸子） intestines 2. （用腸衣塞肉、魚等製成的食品） sausage 3. （感情; 情緒; 情感） heart
  • 核 : 核構詞成分。
  • 結腸 : [生理學] colon; large intesting; col ; coli ; colo 結腸穿刺術 colocentesis; colipuncture; 結腸腹...
  • 結核 : 1. [醫學] tuberculosis; consumption; phthisis; noddle 2. [地質學] concretion3. [礦物學] nodule
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  3. In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm

    發現noea與中慢生根瘤菌（ mesorhizobiumsp . ） bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ，而其303 - 362區域與大腸桿菌（ escherichiacoli ）的核糖體50s亞基的l11蛋白甲基轉移酶（ prma ）的160 - 220結構域的相似性達到41 。
  4. Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian, plant and yeast, etc. the amino acid sequence of the gene shows 99 % ( 179 / 180 ), 98 % ( 177 / 180 ), 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl, triticum aestivum, solatium tuberosum and bos taurus, respectively

    棉花arf基因的同源性分析應用ncbi進行dna序列的相似性分析，結果表明：棉花arf基因與其它植物、動物和酵母的arf基因具有較高的同源性。棉花arf基因編碼的氨基酸序列與擬南芥，小麥、馬鈴薯、牛的同源性分別達到99 （ 179 / 180 ） 、 98 （ 177 180 ） 。 96 （ 174 180 ）楊花腸p核符吞fi曰手谷回伽皿的夭險和92 （ 168ill ） 。
  5. Background : ghrelin is a 28 - amino acid endogenous peptide recently identified in the secretory granules of x / a - like cells in the rat stomach, which can act on the growth hormone secretagogue receptor to accelerate the secretion of growth hormone. it was identified that ghrelin - immunoreactive cell and its receptor localize in areas of brain, such as hypothalamus ( pvn, arc ), hippocampus, adenohypophysis and so on. motilin is a 22 - amino acid peptide secreted from the upper part of the small intestine, which regulates the interdigestive motility of gastric

    背景資料： ghrelin是新發現的由28個氨基酸組成的內源性腦腸肽，由胃部泌酸腺x / a樣內分泌細胞分泌， ghrelin免疫活性神經元及其受體在中樞神經系統中如下丘腦室旁核（ paraventricularneucleus ， pvn ） 、弓狀核（ arcuatenucleus ， arc ） 、海馬區及腺垂體等均有不同濃度的分佈，可與生長素促泌物受體（ growthhormonesecretagoguereceptor ， ghsr ）結合促進生長素（ growthhormone ， gh ）分泌。
  6. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  7. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  8. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  9. Second, a prokaryotic expression construct, obtained from invitrogen transformed into prokaryotic and induced to express vp1 protein. the expressed vp1 fusion protein was purified by affinity chromatography using glutathione - agarose resin and used in elisa and western bolt analysis as the antigen. the elisa and western blot results showed that the anti - fmdv antibody was elicited specifically against vp1 antigen

    第二，為了得到抗原蛋白，將vp1的原核表達質粒pgex - 4t - vp1轉化入大腸桿菌bl21中，經iptg誘導，裂解細胞後用瓊脂糖珠進行純化，用elisa和westernblot進行檢測，結果表明誘導表達出所需大小的融合蛋白。
  10. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。
  11. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  12. The fusion protein had expected primary structure. conclusion : pgex - 4t / mpem expression plasmid was constructed. gst / mpem fusion protein was successfully expressed

    結論：構建了原核融合表達質粒pgex - 4t mpem ；成功在大腸桿菌中表達gst mpem融合蛋白。
  13. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
  14. These anthraquinones are extremely cytotoxic ( they fight against ) towards a broad spectrum of bacteria, fungi, and viruses that include pneumonia, e coli, blood infections, diarrhea, skin infections and ( tuberculosis

    蒽醌是一種極為有效的廣譜類抗細胞毒素物質，可抗擊多種細菌、真菌、病毒，包括導致肺炎的細菌和病毒、大腸埃希氏桿菌，以及導致血液感染、腹瀉、皮膚感染和肺結核的微生物。
  15. The axudl gene consists of five exons and encodes a nuclear protein with 63kd molecular weight. it was frequently down - regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues

    該基因含有五個外顯子，其編碼產物為分子量63kd的細胞核內蛋白，該基因在肺癌、腎癌、肝癌和結腸癌中的表達較相應的正常組織均明顯降低。
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