The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition
熒光標記結合熒光顯微觀察表明:從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微
絲不僅可以直接在熒光顯微鏡下觀察,也可被微
絲的特異標記物鬼筆環
肽所標記,而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光
絲一致;電鏡負染的結果進一步證實其直徑為9nm ,與傳統微
絲直徑相當( 7 ? 10nm ) ;聚合曲線有明顯的停滯期,為典型的s型聚合曲線,聚合臨界濃度為0 . 75 mol l ,這一結果與已有報道相似。
We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants
本研究選取正常棉花的根,已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料,採用石蠟切片,通過fitc -鬼筆環
肽對材料微
絲熒光染色,結合熒光顯微鏡觀察。
Quorum - sensing systems can be divided into two paradigmatic classes : luxi / luxr - type quorum - sensing systems in gram - negative bacteria which produce acyl - homoserine lactone ( ahl ) autoinducers, and oligopeptide / two - component - type quorum - sensing ciruits in gram - positive bacteria which produce autoinducing peptide ( aip ) autoinducers. in contrast to the two paradigmatic quorum - sensing systems, vibrio harveyi produces and responds to an ahl - type autoinducer termed ai - 1 and a novel signal molecule named ai - 2
Qs系統可分為兩種典型的類型,即革蘭氏陰性細菌的luxi luxr型qs系統,革蘭氏陽性細菌的寡
肽雙組分型qs系統,分別以酰基高
絲氨酸內酯( acyl - homoserinelactones , ahl ) 、寡
肽( autoinducingpeptide , aip )為信號分子。
Main ingredient : natural silk power, silk peptide, fresh water pearls, natural fragrance
主要成分:天然
絲素
絲肽淡水珍珠天然香料。
Main ingredient : sodium dna, silk peptide, hyaluronic acid and extract of with hazel
主要成分:活性金植物dha
絲肽透明質酸金縷梅提取物。
Molecular structures of various materials of biological origin, including collagen, silk, bone, protein adhesives, gfp, self - assembling peptides
不同生物材料的分子結構,包括膠原蛋白、
絲蛋白、骨骼、蛋白質附著物、綠色螢光蛋白質、自組裝
肽。
A study on antibacterial activity of antibacterial peptide from lucilia sericata after treatment at high temperature
絲光綠蠅抗菌
肽的熱穩定性研究
There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內
肽酶:巰基蛋白酶、金屬蛋白酶、
絲氨酸蛋白酶,三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置,約為52kd ;前兩種最適ph為6 ,是酸性內
肽酶,后一種有酸( ph6 ) 、堿( ph10 )兩種存在形式,既是酸性內
肽酶又是堿性內
肽酶。
Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested
該酶分子n -端的氨基酸序列為avidtgveashpef ,通過n -端氨基酸序列設計簡並引物,提取被毛孢owvt - 1的總rna , rt - pcr克隆了該酶的成熟
肽編碼基因( 1000bp ) ,序列分析表明,
絲氨酸含量高達12以上。
Pearl silk peptide cream
珍珠
絲肽膏30g
Its main products are silk peptide, pearl and chinese medicinal herb series of cosmetics, silk scream series
公司產品主要以
絲肽,珍珠及中草藥系列天然化妝品,
絲素珍珠系列產品,適合各種膚色人士的使用。
Its main products are silk peptide, pearl and chinese medicinal herb series of cosmetics, silk scream series products, being suitable for the use for people with different skin colors. frequent using these products will soften, tender and smooth your skin so as to prevent the skin from wrinkling and decaying
公司產品主要以
絲肽,珍珠及中草藥系列天然化妝品,
絲素珍珠系列產品,適合各種膚色人士的使用。經常使用使皮膚柔嫩,光滑,起到防皺,延緩衰老作用。
Made of silk peptide and rare chinese herbs. it can be absorbed quickly and have good moisturizing effect on skin
由蠶
絲提取成份
絲素
肽及各種名貴草藥,潤膚劑等多種成份精製而成。
Radiolabeling of filamentous phage peptide library with 99mtc and its biodistribution in normal mice
標記
絲狀噬菌體
肽庫及其在正常小鼠體內的分佈
The content of soluble protein is also increased after deficit water stress, but the increasing of " belami " is more significant than that of " samantha ". 5. after deficit water stress, in " samantha ' petals, the metallo - proteinase activities of the optimum ph 6 and the serine proteinase activities of the optimum ph 10 are increased markedly, the thiol - proteinase activities of the optimum ph 6 is decreased markedly
失水脅迫后顯著提高了『薩蔓莎』花瓣中最適ph6條件下金屬蛋白酶、最適月季( rosahybrde )切花瓶插期間內
肽酶與衰老的關系及失水脅迫對其影響的研究ph條件下的
絲氨酸蛋白酶的活性,顯著降低了最適ph6條件下琉基蛋白酶的活性。
Effects of different leader peptides on the foreign peptide displayed on gene 8 protein of filamentous phage
不同的引導
肽對
絲狀噬菌體基因8蛋白展示外源多
肽的影響
Phage display random peptide library is a kind of polypeptide library developed by filamentous bacteriophages
摘要噬菌體隨機展示肽庫是一類利用絲狀噬菌體發展起來的多肽文庫。