綠色熒光體 的英文怎麼說
中文拼音 [lǚshǎiyíngguāngtǐ]
綠色熒光體
英文
green-emitting phosphor-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。Improvement of genetic transformation system by using gfp as reporter gene on pepper
應用綠色熒光蛋白報告基因優化辣椒的遺傳轉化體系Construction of recombinant adeno - associated virus vector expressing glial cell line - derived neurotrophic factor labeled by green fluorescent protein
綠色熒光蛋白標記的大鼠膠質細胞源性神經營養因子重組腺病毒載體的構建及其表達Production of transgenic embryos expressing green fluorescent protein by nuclear transfer from different types of somatic cells in pig
不同類型的轉基因細胞為核供體生產豬的轉綠色熒光蛋白基因克隆胚胎The symbiosis between mesorhizobium huakuii and astragalus sinicus is a chinese - characteristic symbiotic nitrogen fixation system, while molecular genetic study on its early symbiotic interaction is still at primary stage
本文對新型報告基因?綠色熒光蛋白基因在華癸中生根瘤菌-紫雲英共生固氮體系早期結瘤階段分子遺傳學中的應用進行了探索性研究。Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker
綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。Green fluorescent protein ( gfp ) was extracted from jellyfish ( aequerea victoria ) of ocean invertebrate. gfp can emit green fluorescent when it is illuminated by light of suitable wavelength. the emissions of fluorescence need not any additional factors, such as substrate, supplementary factor
綠色熒光蛋白（ greenfluorescentprotein ， gfp ）是來源於多管水母（ aequoreavictoria ）等海洋無脊椎動物的一種蛋白質，該蛋白質在體外經適當波長的光激發便發出綠色熒光，並且這種熒光的發射不需要任何底物和輔助因子的誘導。In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing
實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。Green fluorescent protein has several good characters. under excitation of long uv light or blue light, it emits green fluorescence without requiring any exogenous substrates and cofactors. gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms. this is a development of revolutionary significance. the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties. the applications of gfp will be wider and wider
綠色熒光蛋白具有優良的特性，在藍光或長紫外光的激發下，不需要任何外源底物或內源輔助因子的參入就能發出綠色熒光.綠色熒光蛋白基因的表達可用來監控活細胞或生物體中基因表達和蛋白質的定位.這是一個革命性的進展.而且，對基因dna序列的改造有可能使綠色熒光蛋白的發光特性更加優良，從而其應用范圍會更加廣泛In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。In the chapter five, the correlation between the intensity of delayed fluorescence and the intactness and function of chloroplast were studied, and the results show that the changes in df intensity of green plants can truly reflect the changes of intactness and functions of chloroplast
第五章系統研究了在酸雨脅迫環境下植物光誘導延遲熒光特性的變化和植物葉片葉綠體數量和功能的改變之間的關系。實驗結果表明:綠色植物葉片光誘導延遲熒光強度的變化能很好地反映植物葉片中完整葉綠體的數量以及葉綠體的功能的變化。Gfp can be expressed in the host enterococcus facecalis and lactobacillus plantarum we isolated using the expression vectors we constructed the expression level depends on the nisin concentration to some extent
Ow洲poi114no例烴們表達載體在我們分離到的受體菌efqecalis和l plantarum中能夠表達綠色熒光蛋白oppx表達的水平依賴於一定范圍濃度的nistn誘導。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
將所構建的單鏈抗體基因克隆入綠色熒光蛋白融合表達載體pegfp - n3 ，瞬時轉染cos - 7細胞，分析其表達情況。Construction and primary application of enhanced green fluorescent protein adenovirus expression vector in the tracking and quantitative analysis of transplanted stem cells
增強型綠色熒光蛋白腺病毒表達載體構建及其在移植幹細胞示蹤和定量中的初步應用Kustaki : ori44, oriw30 and ori2062 were chosen to construct the gfp resolution vector. there were 9 kinds of gfp resolution vector. all of the recombinant plasmids were introduced into crystal negative b. thuringiensis 8mb 171. the fluorescence of the 9 kinds engineered strain can be detected by the fluorescent microscope
將得到的9種gfp解離載體電轉化到蘇雲金芽胞桿菌無晶體突變株bmb171中，熒光顯微鏡鏡檢顯示9株含gfp的蘇雲金芽胞桿菌重組菌在波長為300nm - 510nm的激發光下可發射綠色熒光。分享友人