線狀病毒 的英文怎麼說
中文拼音 [xiànzhuàngbìngdú]
線狀病毒
英文
filovirus- 線 : 名詞1 (用絲、棉、金屬等製成的細長的東西) thread; string; wire 2 [數學] (一個點任意移動所構成的...
- 狀 : Ⅰ名詞1 (形狀) form; shape 2 (情況) state; condition; situation; circumstances 3 (陳述事件或...
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 病毒 : [醫學] virus; inframicrobe (濾過性)
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When he heard professor yang can cure the illness without operate, he came to see professor yang with the last hope. he had the prostatitis and orchitis, the illness was very serious, so not only take orally the powder and capsules but also adopt the fuming and steaming treatment method to fume and wash spermary and perineum. the pain was eased after 3 days, and the symptoms disappeared completely after 2 months
抱著最後一線希望找到楊立華,楊教授為他確診為睪丸炎,前列腺炎,並為他採取薰蒸療法熏洗睪丸與會陰處,同時配合口服藥排出前列腺瘀毒,用藥化解睪丸內瘀腫,通則不痛,痛則不通,三天後睪丸疼痛癥狀減輕了,兩個月病情痊癒。Environmental woes that might be considered catastrophic in some countries can seem commonplace in china : industrial cities where people rarely see the sun ; children killed or sickened by lead poisoning or other types of local pollution ; a coastline so swamped by algal red tides that large sections of the ocean no longer sustain marine life
被一些國家認為災難性的事件的環境污染在中國己經司空見慣:在工業化城市人們極少見到太陽;孩子們因鉛中毒或其他類型的本地化污染而死亡或生病;海岸線被沼泥狀的赤潮所圍困,大部份海洋己經不再適合海洋生物的生存。Different hosts " response suggested that tumv - sd1 could infect plants of 10 species in 3 families. tumv - sd1 formed pine - wheel inclusion bodies in plant cells. the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd, 38kd and 14kd respectively
寄主反應特性表明, tumv - sd1 6能侵染3科10種植物, tumv - sd1在寄主細胞內形成風輪狀內含體,外殼蛋白為3組分,分子量分別為45kd 、 38kd和14kd ;提純的病毒粒體為長線條狀。Much excitement is therefore currently surrounding a new type of virus - - actually a bacterial virus, usually called a phage - - which is circular and double - stranded and which therefore has only a very low tendency to intercalate into other dna at random
因此,令人興奮的是:目前正在圍繞著一種新型的病毒?實際上是一種細菌病毒,通常稱為噬菌體?它是環狀雙線的,因此它隨機插入其他dna的傾向性很低。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外,為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況,我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中,與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒,將其感染sf9細胞制備p1種子液,同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定,確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前,然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中,獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ,並與線性化bm - bacpak6dna共轉染家蠶細胞株,獲得重組病毒bacpak - 6aa - hgm - csf 。Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease, pcr and nested pcr on the basis of the genetic sites of pbluebachisc, which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l
純化該重組質粒並與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 , 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明,獲得了prv - vp7基因與桿狀病毒dna的重組子,命名為a - 1代病毒。Using the result of this study to identify the disease resistance in seed
在電鏡下可觀察到線狀病毒粒子其長度為600 - 750nm 。分享友人