In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用
型） ;鼠單克隆抗體p21waf ， （即用
型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃
縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
Industrial preparing of concentrated lactic acid bacteria starter
濃
縮型乳酸菌發酵劑的工業化生產
For unmagnetized, relativistic and hot ion plasmas, the ion - acoustic waves can be described by the kadomtsev - petviashvili ( kp ) equation ( ( ut + auux + buxxx ) x + duyy = 0 ). it suggests that the nonlinear ion - acoustic solitary waves in a relativistic hot ion plasma are stable even there are some higher order transverse perturbations. there are only compressive solitary waves in the relativistic hot ion plasmas which has been vertified analytically
在低階近似下，無磁場相對論熱離子等離子體可由kp方程（ （ u _ t + auu _ （ x ） + bu _ （ xxx ） _ x + cu _ （ yy ） = 0 ）來描述，相對論熱離子等離子中的非線性離子聲孤波在高階橫向擾動下是穩定的，且在相對論熱離子等離子體中僅存在壓
縮型孤波
To explore and make clear the background factor of the behaviors, the dynamic relationship and cause - effect relationship between organizational politics perceptions and consequential behaviors. this paper, based on the five theories related to power at different levels, personal perspective, strategy contingency theory, social exchange theory and structural theory. there appear several problems in the development of key disciplines and processes of crossing and assimilating of subjects, including system tie, the character of scientists, the problem of administers, which influence the development and construction of subject, through abstracting, summarizing and based on “ swot ” analytical model from the theory strategic competition by michael baud, we divide organizational politics behaviors in the organizational development of college subjects and crossing and assimilating of subjects into several sorts : including ( 1 ) offensive model ( 2 ) defensive model ( 3 ) strengthening model ( 4 ) shrinking model
為探索和理清重點學科組織政治行為的背景因素，組織政治知覺與后續產生的組織政治行為的動態關聯性與因果關系，本研究以五種不同層次與權力相關的理論，即個人特質理論觀點（ personalperspective ） ;策略權變理論觀點（ strategycontingencytheory ） ;沖突理論觀點（ conflicttheory ） ;社會交換理論觀點（ socialexchangytheory ） ;結構化理論觀點（ structurationtheory ）為基本理論依據，將重點學科學科發展以及學科交叉與融合過程中出現的諸如體制束縛、科學家自身素質、管理層問題等影響學科建設與發展的共性現象進行提煉、總結，並依據「邁克爾?波特」的戰略競爭理論學說中的「 swot 」分析模
型，將大學學科組織發展及學科交叉與融合中存在的組織政治行為進行提練歸類，分為（ 1 ）進攻
型; （ 2 ）防守
型; （ 3 ）增強
型; （ 4 ）退
縮型四類政治行為。
Conclusion lu ' s over - extensive pressing manipulations are fairly effective for fractures of thoracic and lumbar vertebrae
結論盧氏過伸緩壓法治療胸腰椎屈曲壓
縮型骨折的療效確切。
Compressed - stranded conductor
壓
縮型絞合導體
Objective to observe the clinical efficacy of lu ' s over - extensive pressing manipulations in the treatment of fractures of thoracic and lumbar vertebrae
摘要目的觀察盧氏過伸緩壓法治療胸腰椎屈曲壓
縮型骨折臨床療效。
Microforms import and export corporation
縮型出版物進出口公司
The twin set crystal posting box series no. 1 and 2 are exquisitely crafted from fine austrian crystal, and each set comprises a larger piece at 30mm and a matching miniature
水晶郵筒?裝系列一號及二號以奧地利優質水晶雕琢而成，每款一套兩個，大郵筒30毫米闊，小郵筒為大的
縮型。
To the northwest area climate, to use the glasgrid which has the next characters : the high tensile strength, the low elongation, the high modulus of elasticity and so on, to absorb the crack of asphalt pavement on a semi - rigid type
計算、實驗室試驗及最終的現場試驗表明，對試驗路? ?寧夏古王高速公路而言，採取加鋪玻璃纖維格柵來降低溫
縮型反射裂縫的措施是非常有效的，其鋪設位置宜在基層頂。
It includes twin set crystal posting box series no. 1 ( left ) and 2 ( right ). the twin set crystal posting box series no. 1 and 2 are exquisitely crafted from fine austrian crystal and each set is comprised of a larger piece at 30mm and a matching miniature
包括水晶郵筒?裝系列一號（圖左）及二號（圖右） ，水晶郵筒?裝系列一號及二號以奧地利優質水晶雕琢而成，每款一套兩個，大郵筒30毫米闊，小郵筒為大的
縮型。
In 1999 several infants suffered a serious complication within two weeks of receiving the vaccine : a segment of the intestine folded into a nearby region ( like a part of a telescope collapses into another ), creating a blockage called intussusception
1999年中，有好幾名嬰兒在服用疫苗后兩周內，出現了嚴重的副作用：有一段腸子出現套疊現象（好比伸
縮型望遠鏡筒的一節
縮入另一節） ，產生稱為腸套疊的阻塞。
Plastics piping systems. joints for glass - reinforced thermosetting plastics pipes and fittings. test methods for leaktightness and resistance to damage of flexible and reduced - articulation joints
塑料管道系統.玻璃纖維增強式熱固性塑料管件和配件接頭.撓性和減
縮型鉸鏈接頭耐泄漏和損壞試驗方法
Abstract : the generalized shrunken prediction of finite population is introduced, using generalized shrunken least squares estimator of linear regression models. with respect to prediction mean squared error, a necessary and sufficient condition for superiority of a generalized shrunken prediction over the best linear unbiased prediction is obtained. in the case of linear combination of every unit index, a linear restricting prediction is introduced and then a necessary and sufficient condition for superiority of linear restricting prediction over the best linear unbiased prediction is devived
文摘:利用線性回歸模型的廣義壓縮最小二乘估計，引入了有限總體的廣義壓縮型預測，在預測均方誤差意義下，得到了廣義壓縮型預測優于最佳線性無偏預測的一個充分必要條件；在只能得到每個個體指標的線性組合時，引入了一種線性約束型預測，並得到了線性約束型預測優于最佳線性無偏預測的一個充分必要條件
