纖維胎體 的英文怎麼說
中文拼音 [xiānwéitāitǐ]
纖維胎體
英文
fabric casing-
Isolation and culture of bovine fetal fibroblasts
牛胎兒成纖維細胞分離與體外培養Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。We get to the conclusions : ( 1 ) the histon acetylation level has some derease in the lung of cloned cattle ; ( 2 ) in the cloned cattle xiaobai, the histone acetylation level is lower than that in normal control, however, higher or adjacent to that in other dead cloned cattle ; ( 3 ) there is no significant difference during those cattle from the cumulus, fetal fibroblast and adult fibroblast cell donor
我們發現： （ 1 ）在克隆牛中組蛋白乙酰化水平基本上都有所降低； （ 2 ）克隆牛xiaobai乙酰化水平比正常牛低，而基本上是高於或接近死亡克隆牛水平的； （ 3 ）在我們所研究的三個供體細胞系（卵丘細胞，成體成纖維細胞和胎兒成纖維細胞）中，乙酰化水平無明顯差異。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。According to the special shape and operate requirements of the torpedo, the structure and moulding process of the torpedo shell are established, in which the body and overlay block with grooves are moulded by fiber winding, and close moulding respectively with the adhesive by limited stop compression. in the light of the selection of raw material system, composite system with outstanding performance are defined, based on which the mechanical properties are tested., with the overall consideration of structural characters and design demands, the main factors which may have effects on the strength and stability ( including stiffness section dimension, stiffeners space, skin thickness and skin lay angle ) is optimized one by one for the structure design parameters on the base of the fem model of skin, stiffness and layover block by fem. as a result, the prototype with 1 : 1 proportion and its structure and lay optimization design are completed. the moulding technology for polyurethane foam moulding the stiffened shell is obtai ned in terms of the design, manufacture moulding, experimental mould and tooling
依據在研產品特殊的外型結構和使用要求，確定了該型號水雷復合材料殼體的具體結構形式和復合成型工藝路線，即由纖維纏繞工藝成型主體異形件，閉模成型帶通槽外貼塊，用限位加壓工裝實現粘接的工藝路線；通過對原材料體系的篩選，確定了綜合性能較好的復合材料體系，以此為基礎進行了力學性能測試；運用有限元方法，建立了蒙皮、筋條和外貼塊的分析模型，綜合考慮結構特點和設計要求，對影響結構強度與穩定性的主要因素（包括筋截面尺寸、筋間距、蒙皮厚度、蒙皮鋪層角度）分別進行了結構設計參數的優選，最終完成了復合材料異形耐外壓殼體1 : 1樣件和縮比實驗件結構及鋪層優化設計；完成了聚氨酯泡沫胎成型內置加筋殼體的成型工藝技術研究；對縮比件進行外壓性能測試，並給出了應力、應變測試結果。The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively
主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。Studies in human fetuses note opioid ( enkephalin - like ) immunoreactie fibers as early as 10 weeks ; moreoer, mu and kappa receptors predominate at birth, with low densities of delta receptors
人類胎兒研究表明，阿片劑（類-腦腓肽）免疫反應纖維在10周左右即存在；而且，和受體在出生時佔主導地位，受體則較少。Studies in human fetuses note opioid ( enkephalin - like ) immunoreactive fibers as early as 10 weeks ; moreover, mu and kappa receptors predominate at birth, with low densities of delta receptors
人類胎兒研究表明，阿片劑（類-腦腓肽）免疫反應纖維在10周左右即存在；而且，和受體在出生時佔主導地位，受體則較少。We also investigated the factors including feeder layer cells, growth factors and complements, which may affect the cultivation and differentiation of these cells in vitro. the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要內容包括： （ 1 ）小鼠胚胎成纖維細胞（ mef ）的分離培養和飼養層細胞的製作以妊娠13 . 5天或14 16天的小鼠為材料，建立了mef分離培養體系。This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide materials to treat various diseases
本研究運用體細胞核移植的原理和技術，以六月齡雌性黑熊的成纖維細胞為供核體，去核未受精家貓成熟卵母細胞為受體，構建種間核移植重組胚胎，研究種問體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide material to treat various diseases
本研究運用體細胞核移植的原理和技術，以成年大鼠的成纖維細胞為供體，去核小鼠和牛成熟卵母細胞為受體，構建種間核移植重組胚胎，研究種間體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。Isolation and culture of murine embryonic fibroblasts of kunming species in vitro
昆明小鼠胚胎成纖維細胞的分離與體外培養分享友人